Results 351 to 360 of about 515,505 (407)

Ultrahigh-throughput Mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops.

Analytical Chemistry, 2013
The behaviors of complex biological systems are often dictated by the properties of their heterogeneous and sometimes rare cellular constituents. Correspondingly, the analysis of individual cells from a heterogeneous population can reveal information not
Dennis J. Eastburn, A. Sciambi, A. Abate
semanticscholar   +1 more source

Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction.

Journal of Clinical Oncology, 1994
PURPOSE Detection of occult carcinoma in patients with breast cancer may aid the establishment of prognosis and development of new therapeutic approaches.
Y. Datta, P. Adams, W. Drobyski, S. Ethier, V. Terry, M. Roth   +5 more
semanticscholar   +1 more source

Prognostic Value of Circulating Melanoma Cells Detected by Reverse Transcriptase–Polymerase Chain Reaction

Journal of Clinical Oncology, 2003
Purpose: Factors that are predictive of prognosis in patients who are diagnosed with malignant melanoma (MM) are widely awaited. Detection of circulating melanoma cells (CMCs) by reverse transcriptase-polymerase chain reaction (RT-PCR) has recently been postulated as a possible negative prognostic factor.
PALMIERI G, ASCIERTO PA, PERRONE F, SATRIANO SMR, OTTAIANO A, DAPONTE A, NAPOLITANO M, CARACÒ C, MOZZILLO N, MELUCCI MT, COSSU A, TANDA F, GALLO, Ciro, SATRIANO RA, CASTELLO G.   +14 more
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Comparison of real-time reverse transcriptase–polymerase chain reaction and nested or commercial reverse transcriptase–polymerase chain reaction for the detection of hepatitis E virus particle in human serum

Diagnostic Microbiology and Infectious Disease, 2006
Hepatitis E virus (HEV) was originally identified as the causative agent of enterically transmitted non-A, non-B hepatitis. The virus is the 7.5-kb single-stranded positive RNA virus and has been classified in the genus Herpevirus [corrected] of the [corrected] Herpeviridae [corrected] Recently, HEVs were identified from several countries worldwide ...
Sang Gyun Kang, Jeong Min Ahn, Han Sang Yoo, Nabin Rayamajhi   +3 more
openaire   +3 more sources

A Reverse Transcriptase-Polymerase Chain Reaction Assay for the Detection and Quantitation of Murine Retroviruses [PDF]

Nature Biotechnology, 1993
Specific hybridization primers for the PCR assay were developed to detect the presence of the ecotropic, xenotropic, and mink cell focus-forming classes of murine leukemia viruses (MuLVs) in samples derived from cultured cells and cell-free supernatants.
Lucille W. S. Chang, Francisco J. Castillo, John M. Irving   +2 more
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Quantification of poliovirus in seawater and sewage by competitive reverse transcriptase - polymerase chain reaction

Canadian Journal of Microbiology, 1998
Reverse transcriptase - polymerase chain reaction (RT-PCR) has been used extensively to detect enteric viruses in environmental samples. Advantages of RT-PCR include its high detection sensitivity and rapid turn-around time. However, unlike traditional cell culture, RT-PCR has not provided quantitation and infectivity information.
Stephen L. Parker, Yu-Li Tsai
openaire   +3 more sources

A Reverse Transcriptase-Polymerase Chain Reaction Assay for Detecting Highlands J Virus

Avian Diseases, 2001
Highlands J (HJ) virus is an arbovirus frequently recovered at high rates in mosquitoes collected in the eastern United States. HJ virus is primarily a veterinary pathogen causing disease in domestic birds including turkeys, chickens, and partridges.
Whitehouse, C. A., Guibeau, A., McGuire, D., Takeda, T., Mather, T. N.   +4 more
openaire   +4 more sources

Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)

2003
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene ...
openaire   +3 more sources

Prognostic significance of cytokeratin-20 reverse transcriptase polymerase chain reaction in lymph nodes of node-negative colorectal cancer patients.

Journal of Clinical Oncology, 2002
PURPOSE Approximately 20% to 30% of patients with curatively resected, node-negative (pN0) colorectal cancer die of tumor recurrence, which can be caused by minimal residual disease.
R. Rosenberg, A. Hoos, James D. Mueller, P. Baier, D. Stricker, M. Werner, H. Nekarda, J. Siewert   +7 more
semanticscholar   +1 more source

Detection of Metastatic Tumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction

2003
In oncology, the correct assessment of tumor stage is crucial information, as it relates to disease-free interval and prognosis. Routine staging modalities use histopathology to evaluate the presence or absence of metastasis mainly in tissues such as lymph nodes, as described in Chapter 1 by Roskell and Buley.
Katja Haack, Michael Neumaier, Krishna Soondrum   +2 more
openaire   +3 more sources