Results 361 to 370 of about 515,505 (407)

Detection and Identification of Flaviviruses by Reverse Transcriptase Polymerase Chain Reaction

1992
We developed a reverse transcriptase/polymerase chain reaction (RT/PCR) for the detection and identification of flavivirus RNA in a single-tube assay. Universal flavivirus RT/PCR amplimers amplify an 832-bp region in the carboxyl portion of NS5 (nt9166-9997) which contains nucleotide sequences conserved among all flaviviruses at the 3′- and 5 ...
Gwong-Jen J. Chang, Dennis W. Trent
openaire   +2 more sources

Virologic features of an astrovirus diarrhea outbreak in a day care center revealed by reverse transcriptase-polymerase chain reaction.

Journal of Infectious Diseases, 1995
Astroviruses cause outbreaks of diarrhea in children attending day care centers (DCCs). Reverse transcriptase-polymerase chain reaction (RT-PCR) was compared with EIA detection of astrovirus in stool specimens to characterize further the molecular ...
D. Mitchell, S. Monroe, X. Jiang, D. Matson, R. Glass, L. Pickering   +5 more
semanticscholar   +1 more source

Amplification of Avian Reovirus RNA Using the Reverse Transcriptase-Polymerase Chain Reaction

Avian Diseases, 1997
A reverse transcriptase-polymerase chain reaction method was developed for the detection of avian reovirus. The origin of primers was from the S1 gene of the avian reovirus genome. A reovirus-specific 532-base pair cDNA product was amplified by these primers from six reference strains and 23 field isolates of avian reoviruses, but not from seven ...
Amin A. Fadl, Mazhar I. Khan, Theodore Girshick, Xie Zhixun   +3 more
openaire   +2 more sources

Assessment of microinvasion with reverse transcriptase polymerase chain reaction in a case of synovial sarcoma

Journal of Orthopaedic Science, 2004
The purpose of our study was to assess microinvasion in a case of synovial sarcoma by means of the reverse transcriptase polymerase chain reaction (RT-PCR) for detecting SYT-SSX fusion transcripts. Furthermore, we tried to compare the RT-PCR results with those obtained by conventional histopathological examination.
Hiroki Gomyo, Hiroyuki Kato, Shinji Wakabayashi, Tominaga Shimizu, Yasuo Yoshimura, Keijiro Mukaiyama, Jun Nakasone   +6 more
openaire   +3 more sources

Prognostic significance of occult metastases detected by sentinel lymphadenectomy and reverse transcriptase-polymerase chain reaction in early-stage melanoma patients.

Journal of Clinical Oncology, 1999
PURPOSE Detection of micrometastases in the regional tumor-draining lymph nodes is critical for accurate staging and prognosis in melanoma patients. We hypothesized that a multiple-mRNA marker (MM) reverse transcriptase-polymerase chain reaction (RT-PCR)
P. Bostick, D. Morton, R. Turner, K. Huynh, He-jing Wang, R. Elashoff, R. Essner, D. Hoon   +7 more
semanticscholar   +1 more source

Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients.

Journal of Clinical Oncology, 1998
PURPOSE This study was performed to evaluate the potential of specific mRNA markers to detect micrometastases by reverse-transcriptase polymerase chain reaction (RT-PCR) and Southern blot analysis of sentinel lymph nodes (SNs) and blood from patients ...
P. Bostick, S. J. Chatterjee, D. Chi, K. Huynh, A. Giuliano, R. Cote, D. Hoon   +6 more
semanticscholar   +1 more source

Detection of the synovial sarcoma translocation t(X;18) (SYT;SSX) in paraffin-embedded tissues using reverse transcriptase-polymerase chain reaction: a reliable and powerful diagnostic tool for pathologists. A molecular analysis of 221 mesenchymal tumors fixed in different fixatives.

Human Pathology, 2001
Synovial sarcoma (SS) is a relatively rare sarcoma, which may be confused with several other mesenchymal and nonmesenchymal lesions. It bears the t(X;18) (SYT;SSX) translocation, which seems to be specific for this tumor type and can be detected in ...
L. Guillou, J. Coindre, Gabrielle Gallagher, P. Terrier, S. Gebhard, N. D. S. Somerhausen, J. Michels, G. Jundt, D. Vince, F. Collin, M. Trassard, V. Doussal, J. Benhattar   +12 more
semanticscholar   +1 more source

Early diagnosis of murray valley encephalitis by reverse transcriptase-polymerase chain reaction

Pathology, 2000
A 4-year-old aboriginal boy developed encephalitis due to Murray Valley encephalitis virus (MVE) following an earlier infection with Kunjin virus (KUN). The illness was severe, resulting in cerebral atrophy and profound physical and intellectual disability.
David W. Smith, Paul G. Carman, Peter C. McMinn   +2 more
openaire   +3 more sources

Prognostic Value of Tyrosinase Reverse-Transcriptase Polymerase Chain Reaction in Metastatic Melanoma

Dermatology, 2001
<i>Background:</i> Circulating tumor cells can now be identified in subjects with metastatic melanoma by using the reverse-transcriptase polymerase chain reaction (RT-PCR) to detect the messenger RNA of tyrosinase, a key enzyme of melanogenesis. <i>Aim and Methods:</i> The aim of this study was to determine whether the detection
Gaëlle Quéreux, Marc G. Denis, Amir Khammari, Brigitte Dréno, P. Lustenberger   +4 more
openaire   +3 more sources

Detection of equine arteritis virus in semen by reverse transcriptase polymerase chain reaction–ELISA

Comparative Immunology, Microbiology and Infectious Diseases, 1999
The reverse transcriptase polymerase chain reaction (RT-PCR) assay was used to detect Equine Arteritis Virus (EAV) in the semen of 88 horses and 2 donkeys, with neutralising antibodies against EAV, on the basis of the amplification of a 279 bp long fragment located in the viral polymerase gene. The RT-PCR assay revealed the virus at 4 TCID50/ml in cell
RAMINA A., DALLA VALLE, LUISA, DE MAS S., TISATO E., ZUIN A., RENIER M., CUTERI V., VALENTE C., CANCELLOTTI F. M.   +8 more
openaire   +4 more sources