The single‐layer RCP‐Chip enables rapid, multiplexed detection of SARS‐CoV‐2 RNA using RT‐LAMP and gold nanoparticle‐enhanced colorimetric readouts. After sample loading, amplification occurs within radially arranged chambers, allowing simultaneous detection of E and N genes.
Pavithra Sukumar+3 more
wiley +1 more source
Evaluation of reverse transcription-polymerase chain reaction and simultaneous amplification and testing for quantitative detection of serum hepatitis B virus RNA. [PDF]
Hu X+8 more
europepmc +1 more source
Short communication: optimization of semi-quantitative RT PCR analysis for CPT I gene expression in rainbow trout (Oncorhynchus mykiss) [PDF]
A key enzyme in mitochondrial β¬oxidation, carnitine palmitoyltransferase (CPT) I, is transcriptionally regulated in mammals, but this enzyme also experiences allosteric modulations (Harano et al., 1985; Murthy and Pande, 1987; Bezaire et al., 2004). CPT
Jalali, S.A.H.+3 more
core
Hispidulin disrupts the KITENIN/ErbB4 oncogenic complex and inhibits KITENIN‐mediated AP‐1 activity, cell invasion, and aerobic glycolysis. It also suppresses the production of cancer‐associated metabolites and downregulates transcriptional regulators downstream of the KITENIN complex.
Mücahit Varlı+7 more
wiley +1 more source
Monitoring of Newcastle Disease Virus Vaccine Strain Replication in Embryonated Chicken Eggs by Reverse Transcription-Polymerase Chain Reaction. [PDF]
Ghadimipour R+5 more
europepmc +1 more source
Mimicking Biochemical Traits with a Synthetic Lipid Nanoparticles SARS‐COV‐2 Model
A synthetic virus model developed using lipid nanoparticles, which incorporate encapsulated mRNA and nucleocapsid (N) protein along with surface‐conjugated spike (S) protein, mimics the characteristics of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), allowing for the advancement of diagnostic product assessment.
Ignasia Handipta Mahardika+7 more
wiley +1 more source
Association of Serum Ferritin With Severity of Disease in Real-Time Reverse Transcription-Polymerase Chain Reaction Negative COVID-19 Patients. [PDF]
Sai Bharath BV+3 more
europepmc +1 more source
Non-Isotopic Competitive Reverse Transcription Polymerase Chain Reaction Coupled with High Performance Liquid Chromatography to Measure β 2 -Receptor Messenger RNA in the Human Heart [PDF]
Klaus Höhnel+6 more
openalex +1 more source
Emerging Therapeutic Strategies for Hearing Loss
Challenges still exist in treating hearing loss in cases of severe damage to hair cells or spiral ganglion neurons. Here, a schematic diagram of cochlear sensory hair cells and auditory nerves is presented. It is found that in normal Corti organs, hair cells have upright stereocilia at the top and auditory neurons at the base. Ototoxic drugs, noise, or
Shanying Han+9 more
wiley +1 more source
Relationship of quantitative reverse transcription polymerase chain reaction (RT-PCR) to RNA Sequencing (RNAseq) transcriptome identifies mouse preimplantation embryo reference genes†. [PDF]
Tscherner AK+3 more
europepmc +1 more source