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PCR, RFLP

2003
Tehnika umnažanja DNA, lančana reakcija polimerazom (PCR), revolucionarna je metoda današnjice. Metoda omogućava eksponencijalno umnažanje ciljnog dijela DNA u uvjetima in vitro. Pokazala se najspecifičnijom i najosjetljivijom za molekularno-genetičke analize. Postoje različiti pristupi samog umnažanja: 1. jednostruki PCR, 2. višestruki PCR, 3.
Grce, Magdalena   +3 more
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RFLP maps of maize

1994
Four molecular maps of the maize genome, with Restriction Fragment Length Polymorphisms (RFLPs), have been presented in documented form (Beavis and Grant 1991; Burr and Burr 1991; Gardiner et al. 1993; J. Shoemaker et al., pers. comm. in Maize Genetics Cooperation News Letter 66: 65–69, 1992).
Edward H. Coe, Jack M. Gardiner
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RFLPS of ApoB Gene

1989
Epidemiological studies have shown1–3 that the plasma concentration of LDL cholesterol and apolipoprotein B-100 are positively correlated with the incidence of coronary artery disease. ApoB-100 is believed to have an important role in the alteration of lipid metabolism that may lead to premature atherosclerosis2,4. Apolipoprotein B-100 is the molecular
F. Turturro   +3 more
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RFLP map of soybean

2001
The soybean is one of the oldest cultivated crops. It first emerged as a domesticated plant around the 11th century B.C. (Hymowitz 1970), although references to soybean appear in books written over 4500 years ago (Smith and Huyser 1987). The soybean was first planted in the United States in Thunderbolt, GA, in 1765 (Hymowitz and Harlan 1983).
Randy C. Shoemaker   +2 more
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RFLP maps of barley

1994
Barley, Hordeum vulgare L., is among the oldest cultivated crops dating 5000 to 7000 years B.C. (Clark 1967; Harlan 1976, 1979) and perhaps much older (Wendorf et al. 1979). Barley has also been a favorite genetic experimental organism since the rediscovery of Mendel’s laws of heredity (Tschermak 1901; cited from Smith 1951).
Andris Kleinhofs, Andrzej Kilian
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RFLP map of peanut

1994
Cultivated peanut (Arachis hypogaea L.) provides a significant source of oil and protein for large segments of the population, particularly in the less developed regions of Asia, Africa, and South America. In the United States, peanut is considered a high-value cash crop of regional importance, with production concentrated in the Southeast region of ...
Tracy Halward   +2 more
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RFLP PCR

2007
Opisana je metoda RFLP-PCR, od engl. Restriction Fragment Length Polymorphism - PCR, koja koristi restrikcijske endonukleaze u reakciji cijepanja odsječaka DNA.
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PCR-RFLP

2020
M. Kam, Jeffrey L. Bidwell
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การตรวจวินิจฉัยแยกชนิดเชื้อบิดในไก่ด้วยวิธี PCR-RFLP

โรคบิดในไก่เป็นโรคโปรโตซัวมีสาเหตุมาจากเชื้อบิดในสกุล Eimeria โดยทั่วไปพื้นฐานของการตรวจแยกชนิดเชื้อบิดในไก่เป็นการแยกจากลักษณะรูปร่างของเชื้อบิดและบริเวณตำแหน่งในลำไส้ของไก่ที่ติดเชื้อบิด ซึ่งจากหลักการนี้พบว่าการตรวจแยกความแตกต่างชนิดของเชื้อบิดในบางครั้งให้ผลไม่น่าเชื่อถือ จึงได้มีการพัฒนานำวิธี Polymerase Chain Reaction-Restriction Fragment Length ...
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