Results 141 to 150 of about 167,361 (378)

Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth. [PDF]

, 1989
We have isolated and sequenced genes from Saccharomyces cerevisiae (SRP54SC) and Schizosaccharomyces pombe (SRP54sp) encoding proteins homologous to both the 54-kD protein subunit (SRP54mam) of the mammalian signal recognition particle (SRP) and the ...
Hann, BC, Poritz, MA, Walter, P
core  

Delayed kinetics of poliovirus RNA synthesis in a human cell line with reduced levels of hnRNP C proteins. [PDF]

, 2010
The hnRNP C heterotetramer [(C1(3))C2] binds RNA polymerase II transcripts in the nucleus, along with other proteins of the core hnRNP complex, and plays an important role in mRNA biogenesis and transport.
Brunner, Jo Ellen, Ertel, Kenneth J, Rozovics, Janet M, Semler, Bert L   +3 more
core   +1 more source

TriCON: A Carbon‐Based Triple‐Modal Nanoplatform for Pancreatic Cancer Therapy

Advanced Science, EarlyView.
We developed TriCON, a triple‐modality nanotherapeutic platform, to treat pancreatic ductal adenocarcinoma (PDAC) by synergizing gene editing, chemotherapy, and immunotherapy. TriCON utilizes CRISPR/Cas9 to target the poliovirus receptor (PVR), combined with nano‐encapsulated doxorubicin and checkpoint blockade. This approach achieved significant tumor
Xinyu Peng, Jiaxing Huang, Shengnan Lv, Jian Zhang, Liangliang Zhang, Huan Liu, Yan Liu, Zhen‐An Qiao, Feng Wei, Bao‐Lian Su   +9 more
wiley   +1 more source

Dual Targeting of Mutant p53 and SNRPD2 via Engineered Exosomes Modulates Alternative Splicing to Suppress Ovarian Cancer

Advanced Science, EarlyView.
Mutant p53 drives oncogenic splicing to promote the progression of ovarian cancer by partnering with the spliceosome factor SNRPD2. Therefore, it is engineered iRGD‐exosomes to co‐deliver siRNAs against both targets. This approach restored tumor‐suppressive mRNA isoforms, effectively enhanced sensitivity to cisplatin, and ultimately blocked tumor ...
Wei Zhao, Qian Hao, Yu Gan, Jing Tong, Xiaodan Chen, Shuran Tan, Ruiwen Ruan, Yingdan Huang, Mingming Cao, Jun Deng, Tao Han, Getao Shi, Bo Gao, Yu Zhang, Xiang Zhou   +14 more
wiley   +1 more source

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

BMC Biotechnology, 2020
Background Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80–90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools and protocols are only ...
Oliver Blechert, Huan Mei, Xiaohui Zang, Hailin Zheng, Guanzhao Liang, Weida Liu   +5 more
doaj   +1 more source

Systematic Discovery of Endogenous Human Ribonucleoprotein Complexes

bioRxiv, 2018
Ribonucleoprotein (RNP) complexes are important for many cellular functions but their prevalence has not been systematically investigated. We developed a proteome-wide fractionation-mass-spectrometry strategy called differential fractionation (DIF-FRAC ...
Anna L. Mallam, Wisath Sae-Lee, Jeffrey M. Schaub, Fan Tu, Anna M. Battenhouse, Y. Jang, Jonghwan Kim, Ilya J. Finkelstein, E. Marcotte, K. Drew   +9 more
semanticscholar   +1 more source

CircTspan3 Promotes Cartilage Development Through ANNEXIN A2‐Mediated Ferroptosis and Apoptosis Inhibition and Exosome‐Mediated Paracrine Signaling

Advanced Science, EarlyView.
This study reveals that XBP1s drives production of circTspan3, a circular RNA that strengthens cartilage by boosting anabolic activity and limiting cell death. Phosphorylated ANXA2 directs circTspan3 into exosomes, enabling paracrine repair. Exosomal circTspan3 expands growth‐plate cartilage and promotes in vivo regeneration, highlighting its promise ...
Yiming Pan, Fengmei Zhang, Jiayan Zhong, Qian Gong, Nana Geng, Biao Kuang, Qiumei Lan, Miao Yi, Qiqi Zeng, Cheng Chen, Mengtian Fan, Chunliang Zhao, Deping Zeng, Yu Du, Mao Nie, Zhibiao Wang, Fengjin Guo   +16 more
wiley   +1 more source

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes*

Journal of Biological Chemistry, 2016
The CRISPR/Cas9 system has been employed to efficiently edit the genomes of diverse model organisms. CRISPR-mediated mouse genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and single guide RNA (sgRNA) into zygotes to generate ...
Sean Chen, Benjamin Lee, Angus Y. Lee, A. Modzelewski, Lin He   +4 more
semanticscholar   +1 more source

CRISPR in MOF Formulation with Enhanced Stability, Activity, and Altered PAM Specificity for Broad‐Spectrum Diagnosis of Bacterial Sepsis

Advanced Science, EarlyView.
CRISPR‐FLEXMO (CRISPR with flexible PAM in MOF encapsulation) integrates a PAM‐relaxed Cas12a K607R enzyme with a manganese‐coordinated MOF for broad‐spectrum diagnosis of bacterial sepsis. The MOF enhances activity and preserves functionality under heat, solvent, and chaotropic stress, supporting long‐term room‐temperature storage.
Tathagata Pal, Zilong Liu, Meera G. Nair, Juhong Chen   +3 more
wiley   +1 more source

Identification of an In Vitro Ribonucleoprotein Complex Between a Viroid RNA and a Phloem Protein from Cucumber Plants

Molecular Plant-Microbe Interactions, 2001
We used the interaction of Hop stunt viroid (HSVd) and cucumber plants to investigate the involvement of phloem proteins in the systemic transport of RNA molecules.
Gustavo Gómez, Vicente Pallás
doaj   +1 more source