Results 141 to 150 of about 144,340 (386)

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

BMC Biotechnology, 2020
Background Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80–90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools and protocols are only ...
Oliver Blechert, Huan Mei, Xiaohui Zang, Hailin Zheng, Guanzhao Liang, Weida Liu   +5 more
doaj   +1 more source

SF3A3 Drives Tumorigenesis in Endometrial Cancer by Enhancing c‐FOS Expression and Represents a Potential Therapeutic Target

Advanced Science, EarlyView.
The role of splicing factor 3A subunit 3 (SF3A3) in driving endometrial cancer (EC) progression and potential therapeutic interventions. SF3A3, a core component of the U2 small nuclear ribonucleoprotein (U2 snRNP), is upregulated in endometrial cancer (EC) and promotes tumor growth and cisplatin resistance. RNA‐binding protein immunoprecipitation (RIP)
Wei Yu, Yi Gu, Hengrui Zhang, Wunan Huang, Lei Sun, Liangjian Ma, Xiaolei Liang, Lexin Liu, Guiping Chen, Weihua Wang, Zhongkai Cao, Xue Li, Xiangjun Chen, Lidan Hu   +13 more
wiley   +1 more source

Identification of an In Vitro Ribonucleoprotein Complex Between a Viroid RNA and a Phloem Protein from Cucumber Plants

Molecular Plant-Microbe Interactions, 2001
We used the interaction of Hop stunt viroid (HSVd) and cucumber plants to investigate the involvement of phloem proteins in the systemic transport of RNA molecules.
Gustavo Gómez, Vicente Pallás
doaj   +1 more source

EFTUD2 Regulates Cortical Morphogenesis via Modulation of Caspase‐3 and Aifm1 Splicing Pathways

Advanced Science, EarlyView.
EFTUD2, a spliceosomal GTPase linked to MFDM, regulates cortical development through apoptotic control. Conditional Eftud2 knockout in murine neural stem cells induces microcephaly and cortical disorganization, while pathogenic variants drive neuronal loss.
Liping Chen, Ying Li, Yan Yu, Mingze Cai, Hao Li, Minghe Huang, Guochao Yang, Jiageng Guo, Huailin Wang, Zhihong Song, Wei Shen, Huihui Jiang, Haitao Wu   +12 more
wiley   +1 more source

The multistructural forms of box C/D ribonucleoprotein particles

RNA: A publication of the RNA Society, 2018
Structural biology studies of archaeal and yeast box C/D ribonucleoprotein particles (RNPs) reveal a surprisingly wide range of forms. If form ever follows function, the different structures of box C/D small ribonucleoprotein particles (snoRNPs) may ...
Ge Yu, Yu Zhao, Hong Li
semanticscholar   +1 more source

PCSK9 Loss‐of‐Function Disrupts Cellular Microfilament Network via LIN28A/HES5/JMY Axis in Neural Tube Defects

Advanced Science, EarlyView.
PCSK9 acts as a molecular chaperone promoting LIN28A lysosomal degradation. LIN28A elevates transcription factor HES5, increasing JMY expression. PCSK9 loss causes neural tube defects (NTDs) by disrupting the LIN28A/HES5/JMY axis, and high JMY disorganizes the neural progenitor cell microfilament network, leading to incomplete neural tube structure in ...
Xiaoshuai Li, Rui Wang, Wenting Luo, Hui Gu, Tianchu Huang, Qiushi Wang, Zhengwei Yuan   +6 more
wiley   +1 more source

Development of a CRISPR/Cas9-Mediated Gene Knockout Method for Functional Genomics of the Barley Spot Blotch Pathogen Bipolaris sorokiniana

PhytoFrontiers
Bipolaris sorokiniana (=Cochliobolus sativus) is an important fungal pathogen that causes spot blotch, common root rot, and kernel blight in barley and wheat. In this study, we aimed to develop a CRISPR/Cas9-mediated approach for efficiently knocking out
Alireza Poursafar, Yueqiang Leng, Shaobin Zhong   +2 more
doaj   +1 more source

Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth. [PDF]

, 1989
We have isolated and sequenced genes from Saccharomyces cerevisiae (SRP54SC) and Schizosaccharomyces pombe (SRP54sp) encoding proteins homologous to both the 54-kD protein subunit (SRP54mam) of the mammalian signal recognition particle (SRP) and the ...
Hann, BC, Poritz, MA, Walter, P
core  

Formation of Particulate Ribonucleoprotein with Enzymic Activities in vitro. [PDF]

, 1958
Agnar P. Nygaard, Veikko Nurmikko, K. Hartiala, K. Hartiala, S. Veige, E. Diczfalusy   +5 more
openalex   +1 more source

Lactylation of HMGB1 at K177 Drives Nuclear Export of TIAR to Promote Hypoxia‐Induced Stress Granule Formation

Advanced Science, EarlyView.
This study uncovers a novel mechanism in which p300‑catalyzed lactylation of HMGB1 triggers nuclear export of the HMGB1‐TIAR complex, driving TIAR‐dependent SGs formation in the cytoplasm. Mass spectrometry and mutagenesis reveals that K177 lactylation is essential for this export and subsequent SGs formation.
Chengyu Li, Zhaojun Liu, Linjie Zhu, Gang Wu, Chen Fu, Hongmin Li, Tong He, Ming Shen, Honglin Liu   +8 more
wiley   +1 more source