Results 311 to 320 of about 144,340 (386)

Transcriptional gene fusions via targeted integration at safe harbors for high transgene expression in Chlamydomonas reinhardtii

New Phytologist, EarlyView.
Summary Conventional genetic engineering in green microalgae employs error‐prone nonhomologous end joining to integrate recombinant DNA at double‐strand breaks generated at random positions across the nuclear genome. This typically results in variable transcription strength and requires a labor‐intensive screening procedure to identify transformants ...
Nick Jacobebbinghaus, Florian Bigge, Merve Saudhof, Wolfgang Hübner, Olaf Kruse, Thomas Baier   +5 more
wiley   +1 more source

The role of telomere and telomerase in cancer and novel therapeutic target: narrative review. [PDF]

Front Oncol
Baylie T, Jemal M, Baye G, Getinet M, Amare GA, Adugna A, Abebaw D, Hibstu Z, Tegegne BA, Gugsa E, Adane T, Getie G, Ashenef B, Sinamaw D.   +13 more
europepmc   +1 more source

RNA elements and their biotechnological applications in plants

New Phytologist, EarlyView.
Summary Engineering of plants for improved traits and efficient heterologous protein production can be achieved by modifying or introducing cis‐ or trans‐acting RNA elements. The function of these elements depends not only on their nucleotide sequence but also on their highly dynamic higher order structures.
Filip Lastovka, Hadrien Peyret, George P. Lomonossoff, Betty Y.‐W. Chung   +3 more
wiley   +1 more source

RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma. [PDF]

Hereditas
Geng Y, Yang L, Shao R, Xu T, Zhang L.
europepmc   +1 more source

From photoprotection to plasticity: transposon activation in the Chlamydomonas det1 mutant

New Phytologist, EarlyView.
Summary Transposable elements (TEs) contribute to genomic adaptation but are typically silenced to maintain genome integrity. In this study, we investigated TE activation in a Chlamydomonas reinhardtii mutant deficient in DE‐ETIOLATED1 (DET1).
Konomi Fujimura‐Kamada, Jun Minagawa
wiley   +1 more source

Transcriptomic profiling of unmethylated full mutation carriers implicates TET3 in FMR1 CGG repeat expansion methylation dynamics in fragile X syndrome. [PDF]

J Neurodev Disord
Farmiloe G, Bejczy V, Tabolacci E, Willemsen R, Jacobs F.   +4 more
europepmc   +1 more source

The Genetic Blueprint of Obesity: From Pathogenesis to Novel Therapies

Obesity Reviews, EarlyView.
ABSTRACT Obesity is a chronic metabolic disease characterized by disturbances in energy homeostasis, leading to excessive fat accumulation. The pathogenesis of the disease is shaped by a complex interplay of genetic, epigenetic, biological, psychological, and environmental factors.
Gašper Tonin, Stjepan Eržen, Zala Mlinarič, Dubravka Jurišić Eržen, Simon Horvat, Tanja Kunej, Jasna Klen   +6 more
wiley   +1 more source

Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing in <i>Ganoderma lucidum</i>. [PDF]

Bioengineered
Eom H, Choi YJ, Nandre R, Kim M, Oh YL, Kim S, Nakazawa T, Honda Y, Ro HS.   +8 more
europepmc   +1 more source

Highly efficient transgene‐free ErCas12a RNP‐protoplast genome editing and single‐cell regeneration in Nicotiana benthamiana for glyco‐engineering

Plant Biotechnology Journal, EarlyView.
Summary Nicotiana benthamiana serves as a unique platform for biopharmaceutical production, offering advantages such as efficient and scalable protein synthesis. In addition, custom N‐glycans can be engineered on biopharmaceutical glycoproteins. Yet, plant‐native glycosyltransferases and glycoside hydrolases need to be removed to prevent undesired ...
Lukas C. Blumberg, Geartsje M. Bakker, Alex van der Kaaij, Élyse Gariépy, Henri van de Geest, Erik J. Slootweg, Ferdinand C. O. Los, Lotte B. Westerhof, Ruud H. P. Wilbers   +8 more
wiley   +1 more source

CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR-15b-5p and miR-7-1-3p processing in nasopharyngeal carcinoma. [PDF]

Mol Oncol
Fan C, Tan F, Wu J, Zeng Z, Guo W, Huang H, Xiong W.   +6 more
europepmc   +1 more source