Results 71 to 80 of about 144,340 (386)

Reentrant Phase Transition Drives Dynamic Substructure Formation in Ribonucleoprotein Droplets.

Angewandte Chemie, 2017
Intracellular ribonucleoprotein (RNP) granules are membrane-less droplet organelles that are thought to regulate posttranscriptional gene expression. While liquid-liquid phase separation may drive RNP granule assembly, the mechanisms underlying their ...
Priya R Banerjee, Anthony N Milin, M. M. Moosa, Paulo L Onuchic, A. Deniz   +4 more
semanticscholar   +1 more source

The human Dicer helicase domain is capable of ATP hydrolysis and single-stranded nucleic acid binding

BMC Biology
Background Vertebrates have one Dicer ortholog that generates both microRNAs (miRNAs) and small interfering RNAs (siRNAs), in contrast to the multiple Dicer-like proteins found in flies and plants.
Kinga Ciechanowska, Agnieszka Szczepanska, Kamil Szpotkowski, Klaudia Wojcik, Anna Urbanowicz, Anna Kurzynska-Kokorniak   +5 more
doaj   +1 more source

RNA-binding protein immunoprecipitation as a tool to investigate plant miRNA processing interference by regulatory proteins of diverse origin [PDF]

, 2018
Background: Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways.
Garcia, Maria Laura, Marmisollé, Facundo Ernesto, Reyes Martinez, Carina Andrea   +2 more
core   +2 more sources

High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes

Genetics, 2015
Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based expression of Cas9 and the gene-specific guide RNAs.
Alexandre Paix, Andrew W. Folkmann, D. Rasoloson, G. Seydoux   +3 more
semanticscholar   +1 more source

Possible role of human ribonuclease dicer in the regulation of R loops

FEBS Open Bio, EarlyView.
R loops play an important role in regulating key cellular processes such as replication, transcription, centromere stabilization, or control of telomere length. However, the unscheduled accumulation of R loops can cause many diseases, including cancer, and neurodegenerative or inflammatory disorders. Interestingly, accumulating data indicate a possible
Klaudia Wojcik, Paulina Krzeminska, Anna Kurzynska‐Kokorniak   +2 more
wiley   +1 more source

RNA-dependent association with myosin IIA promotes F-actin-guided trafficking of the ELAV-like protein HuR to polysomes [PDF]

, 2013
The role of the mRNA-binding protein human antigen R (HuR) in stabilization and translation of AU-rich elements (ARE) containing mRNAs is well established.
Doller, Anke, Eberhardt, Wolfgang, Pfeilschifter, Josef Martin, Schulz, Sebastian   +3 more
core   +1 more source

Long non‐coding RNAs as therapeutic targets in head and neck squamous cell carcinoma and clinical application

FEBS Open Bio, EarlyView.
Long non‐coding RNAs (lncRNAs) occupy an abundant fraction of the eukaryotic transcriptome and an emerging area in cancer research. Regulation by lncRNAs is based on their subcellular localization in HNSCC. This cartoon shows the various functions of lncRNAs in HNSCC discussed in this review.
Ellen T. Tran, Ruchi A. Patel, Amogh Chariyamane, Ratna B. Ray   +3 more
wiley   +1 more source

Efficient multi-allelic genome editing via CRISPR–Cas9 ribonucleoprotein-based delivery to Brassica napus mesophyll protoplasts

Frontiers in Plant Science
Canola (Brassica napus L.) is a valuable oilseed crop worldwide. However, trait improvement by breeding has been limited by its low genetic diversity and polyploid genetics.
Sareena Sahab, Fatima Runa, Mahilini Ponnampalam, Pippa T. Kay, Elizabeth Jaya, Katerina Viduka, Stephen Panter, Josquin Tibbits, Josquin Tibbits, Matthew J. Hayden, Matthew J. Hayden   +10 more
doaj   +1 more source

Characterization of LINE-1 ribonucleoprotein particles. [PDF]

PLoS Genetics, 2010
The average human genome contains a small cohort of active L1 retrotransposons that encode two proteins (ORF1p and ORF2p) required for their mobility (i.e., retrotransposition).
Aurélien J Doucet, Amy E Hulme, Elodie Sahinovic, Deanna A Kulpa, John B Moldovan, Huira C Kopera, Jyoti N Athanikar, Manel Hasnaoui, Alain Bucheton, John V Moran, Nicolas Gilbert   +10 more
doaj   +1 more source

Characterization of a disease-associated mutation affecting a putative splicing regulatory element in intron 6b of the cystic fibrosis transmembrane conductance regulator (CFTR) gene [PDF]

, 2009
Cystic fibrosis (CF) is a common recessive disorder caused by >1600 mutations in the CF transmembrane conductance regulator (CFTR) gene. About 13% of CFTR mutations are classified as “splicing mutations,” but for almost 40% of these, their role in ...
BAFFICO AM, CAO A, CORDA D, FAA' V, INCANI, FEDERICA, MASALA, MADDALENA, MELONI A, ROSATELLI, MARIA CRISTINA, SEIA M   +8 more
core   +1 more source