Results 51 to 60 of about 26,264 (280)

Immunoprecipitation and Sequencing of Acetylated RNA

Bio-Protocol, 2019
Generation of the epitranscriptome through chemical modifications of protein-coding messenger RNAs (mRNAs) has emerged as a new mechanism of post-transcriptional gene regulation.
Daniel Arango, David Sturgill, Shalini Oberdoerffer   +2 more
doaj   +1 more source

Synthesis of 2‐Substituted Adenosine Triphosphate Derivatives and their use in Enzymatic Synthesis and Postsynthetic Labelling of RNA

ChemBioChem, EarlyView.
A series of 2‐substituted adenosine 5′‐O‐triphosphate derivatives is synthesized and tested as substrates for polymerases. In vitro transcription with T7 RNA polymerase, primer extension with engineered DNA polymerases, or 3′‐labelling with poly(A) polymerase are used for enzymatic synthesis of base‐modified RNA.
Ugnė Šinkevičiūtė, Tania Sanchez‐Quirante, Samanta Rožánková, Lenka Poštová Slavětínská, Veronika Raindlová, Michal Hocek   +5 more
wiley   +1 more source

Salvage enzymes in nucleotide biosynthesis [PDF]

, 2007
Balanced pools of deoxyribonucleoside triphosphates (dNTPs), the building blocks of DNA, and ribonucleoside triphosphates (NTPs), the precursors of RNA, are crucial for a controlled cell proliferation.
Kosinska, Urszula
core  

Combinatorial omics analysis reveals perturbed lysosomal homeostasis in collagen VII-deficient keratinocytes [PDF]

, 2018
The extracellular matrix protein collagen VII is part of the microenvironment of stratified epithelia and critical in organismal homeostasis. Mutations in the encoding gene COL7A1 lead to the skin disorder dystrophic epidermolysis bullosa (DEB), are ...
Athanasiou, Ioannis, Backofen, Rolf, Bornert, Olivier, Bruckner-Tuderman, Leena, Dengjel, Jörn, Erxleben, Anika, Grüning, Björn Andreas, Has, Cristina, Kiritsi, Dimitra, Leppert, Juna, Nyström, Alexander, Reinheckel, Thomas, Thriene, Kerstin, Weber, Ekkehard   +13 more
core   +1 more source

Mechanochemical Reactivity of Ribonucleosides Mediated by Inorganic Species: Implications for Extraterrestrial Organic Matter Interpretation

Applied Sciences
Metal species and carbonate are often found as minerals in extraterrestrial rocky bodies. Based on this, the mechanochemical-induced degradation of canonical purine and pyrimidine ribonucleosides into their corresponding nucleobases mediated by some of ...
Gustavo P. Maia, Catarina Gonçalves, Ana J. Carvalho, Vânia André, Adelino Galvão, Ana P. C. Ribeiro, Pedro F. Pinheiro, José Armando Luísa da Silva   +7 more
doaj   +1 more source

AICAr, a Widely Used AMPK Activator with Important AMPK-Independent Effects: A Systematic Review

Cells, 2021
5-Aminoimidazole-4-carboxamide ribonucleoside (AICAr) has been one of the most commonly used pharmacological modulators of AMPK activity. The majority of early studies on the role of AMPK, both in the physiological regulation of metabolism and in cancer ...
Dora Višnjić, Hrvoje Lalić, Vilma Dembitz, Barbara Tomić, Tomislav Smoljo   +4 more
doaj   +1 more source

Synthesis of Nucleoside Derivatives by Biomimetic Ester Migration

ChemBioChem, Accepted Article.
Modified nucleosides play important roles as agents in medicinal chemistry due to their anti‐inflammatory, antiviral and anti‐proliferative properties, as well as in biochemical processes like protein biosynthesis. Aminoacylated nucleosides in tRNA represent the central transfer unit of amino acids in the biosynthesis of peptides.
Oliver Trapp, Nathalie J. Kurrle, Christoph J. B. Seifert, Nathalie Hampel, Tamara Rauch, Michael Thoma, Luca V. Parziale, Marian S. R. Ebeling, Dino Berthold   +8 more
wiley   +1 more source

Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A) [PDF]

, 1977
A template-dependent RNA polymerase has been isolated from poliovirus-infected cells by assaying for the ability of the enzyme to copy poly(A) complexed to an oligo(U) primer.
Baltimore, David, Flanegan, James B.
core  

Polymerase Synthesis of Hypermodified DNA Displaying a Combination of Thiol, Hydroxyl, Carboxylate, and Imidazole Functional Groups in the Major Groove

Chemistry – A European Journal, EarlyView.
A series of 2’‐deoxyribonucleoside 5’‐O‐triphosphates bearing functional groups of amino acid side chains in enzyme active sites (OH, SH, COOH, and imidazole) were synthesized and used for enzymatic synthesis of hypermodified DNA displaying these substituents in the major groove mimicking peptides or proteins. Abstract We designed and synthesized a set
Lukáš Kaiser, Marek Ondruš, Lenka Poštová Slavětínská, Veronika Raindlová, Michal Hocek   +4 more
wiley   +1 more source

C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins [PDF]

, 2014
An expanded GGGGCC repeat in C9orf72 is the most common genetic cause of frontotemporal dementia and amyotrophic lateral sclerosis. A fundamental question is whether toxicity is driven by the repeat RNA itself and/or by dipeptide repeat proteins ...
Cabecinha, M, Clayton, EL, Cleverley, K, Devoy, A, Dols, J, Fisher, EMC, Fratta, P, Grönke, S, Hendrich, O, Isaacs, AM, Mizielinska, S, Moens, T, Niccoli, T, Nicoll, AJ, Norona, FE, Partridge, L, Pickering-Brown, S, Pietrzyk, J, Ridler, CE, Woollacott, IOC   +19 more
core   +1 more source