Enhanced subunit interactions with gemcitabine-5'-diphosphate inhibit ribonucleotide reductases. [PDF]
Proc Natl Acad Sci U S A, 2007 Wang J, Lohman GJ, Stubbe J.
europepmc +2 more sources
Convergent Allostery in Ribonucleotide Reductase [PDF]
Nature Communications, 2018 AbstractRibonucleotide reductases (RNRs) use a conserved radical-based mechanism to catalyze the conversion of ribonucleotides to deoxyribonucleotides. Within the RNR family, class Ib RNRs are notable for being largely restricted to bacteria, including many pathogens, and for lacking an evolutionarily mobile ATP-cone domain that allosterically controls
William C. Thomas +7 more
openaire +3 more sources
The anaerobic (class III) ribonucleotide reductase from Lactococcus lactis. Catalytic properties and allosteric regulation of the pure enzyme system [PDF]
, 2000 Lactococcus lactis contains an operon with the genes (nrdD and nrdG) for a class III ribonucleotide reductase. Strict anaerobic growth depends on the activity of these genes. Both were sequenced, cloned, and overproduced in Escherichia coli.
Buist, Girbe +7 more
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Measurement of FNR-NrdI Interaction by Microscale Thermophoresis (MST)
Bio-Protocol, 2017 This protocol describes how to measure protein-protein interactions by microscale thermophoresis (MST) using the MonolithTM NT.115 instrument (NanoTemper).
Ingvild Gudim +3 more
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eLife, 2022 Ribonucleotide reductases (RNRs) are used by all free-living organisms and many viruses to catalyze an essential step in the de novo biosynthesis of DNA precursors. RNRs are remarkably diverse by primary sequence and cofactor requirement, while sharing a
Andrew A Burnim +4 more
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Spd2 assists Spd1 in modulation of RNR architecture but does not regulate deoxynucleotide pools [PDF]
, 2014 In yeasts, small intrinsically disordered proteins (IDPs) modulate ribonucleotide reductase (RNR) activity to ensure an optimal supply of dNTPs for DNA synthesis. The S. pombe Spd1 protein can directly inhibit the large RNR subunit (R1), import the small
Basrai +37 more
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Role of RRM1 in the Treatment and Prognosis of Advanced Non-small Cell Lung Cancer
Chinese Journal of Lung Cancer, 2015 Lung cancer is one of the most common and highest mortality rates malignant tumors, further, 75%-80% is non-small cell lung cancer (NSCLC). For the majority of patients, lost the chance of radical operation or radiotherapy, chemotherapy is the main ...
Jiawei TIAN, Shuhua HAN
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50 Years of Giese Reaction – a Personal View
Angewandte Chemie, EarlyView.The Giese‐Reaction celebrates its 50. Birthday, it is a cyclic radical chain reaction centered around C,C‐bond formation in a three component synthesis. This minireview speaks about the birth of the name‐reaction, explains how the complete understanding of its reaction mechanism stimulates new methods in organic synthesis, and why it can proceed in ...
Martin Spichty +4 more
wiley +2 more sources
Thioredoxin reductase from Bacillus cereus exhibits distinct reduction and NADPH‐binding properties
FEBS Open Bio, 2021 Low‐molecular‐weight (low Mr) thioredoxin reductases (TrxRs) are homodimeric NADPH‐dependent dithiol flavoenzymes that reduce thioredoxins (Trxs) or Trx‐like proteins involved in the activation networks of enzymes, such as the bacterial class Ib ...
Marita Shoor +3 more
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Yeast Dun1 Kinase Regulates Ribonucleotide Reductase Small Subunit Localization in Response to Iron Deficiency [PDF]
, 2016 Ribonucleotide reductase (RNR) is an essential iron-dependent enzyme that catalyzes deoxyribonucleotide synthesis in eukaryotes. Living organisms have developed multiple strategies to tightly modulate RNR function to avoid inadequate or unbalanced ...
An, Xiuxiang +6 more
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