Results 41 to 50 of about 181,495 (258)

RNRdb, a curated database of the universal enzyme family ribonucleotide reductase, reveals a high level of misannotation in sequences deposited to Genbank

open access: yesBMC Genomics, 2009
Background Ribonucleotide reductases (RNRs) catalyse the only known de novo pathway for deoxyribonucleotide synthesis, and are therefore essential to DNA-based life. While ribonucleotide reduction has a single evolutionary origin, significant differences
Poole Anthony M   +3 more
doaj   +1 more source

The class III ribonucleotide reductase from Neisseria bacilliformis can utilize thioredoxin as a reductant [PDF]

open access: yes, 2014
The class III anaerobic ribonucleotide reductases (RNRs) studied to date couple the reduction of ribonucleotides to deoxynucleotides with the oxidation of formate to CO[subscript 2]. Here we report the cloning and heterologous expression of the Neisseria
Baek, Jiyeon   +5 more
core   +1 more source

Aerobic Vitamin B12 Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth

open access: yesFrontiers in Microbiology, 2018
Pseudomonas aeruginosa is a major pathogenic bacterium in chronic infections and is a model organism for studying biofilms. P. aeruginosa is considered an aerobic bacterium, but in the presence of nitrate, it also grows in anaerobic conditions.
Anna Crespo   +2 more
doaj   +1 more source

Function of the Pseudomonas aeruginosa NrdR Transcription Factor: Global Transcriptomic Analysis and Its Role on Ribonucleotide Reductase Gene Expression. [PDF]

open access: yesPLoS ONE, 2015
Ribonucleotide reductases (RNRs) are a family of sophisticated enzymes responsible for the synthesis of the deoxyribonucleotides (dNTPs), the building blocks for DNA synthesis and repair. Although any living cell must contain one RNR activity to continue
Anna Crespo   +2 more
doaj   +1 more source

Bacillus Subtilis Class Ib Ribonucleotide Reductase: High Activity and Dynamic Subunit Interactions [PDF]

open access: yes, 2013
The class Ib ribonucleotide reductase (RNR) isolated from Bacillus subtilis was recently purified as a 1:1 ratio of NrdE (α) and NrdF (β) subunits and determined to have a dimanganic-tyrosyl radical (Mn[superscript III][subscript 2]-Y·) cofactor.
Parker, Mackenzie James   +2 more
core   +1 more source

Genomics, Transcriptomics, and Metabolomics Reveal That Minimal Modifications in the Host Are Crucial for the Compensatory Evolution of ColE1-Like Plasmids

open access: yesmSphere, 2022
Plasmid-mediated antimicrobial resistance is one of the major threats to public health worldwide. The mechanisms involved in the plasmid/host coadaptation are still poorly characterized, and their understanding is crucial to comprehend the genesis and ...
Manuel Ares-Arroyo   +7 more
doaj   +1 more source

The Manganese-containing Ribonucleotide Reductase of Corynebacterium ammoniagenes is a Class Ib Enzyme [PDF]

open access: yes, 1998
Ribonucleotide reductases (RNRs) are key enzymes in living cells that provide the precursors of DNA synthesis. The three characterized classes of RNRs differ by their metal cofactor and their stable organic radical.
Barbé García, Jordi   +8 more
core   +2 more sources

Novel ATP-cone-driven allosteric regulation of ribonucleotide reductase via the radical-generating subunit

open access: yeseLife, 2018
Ribonucleotide reductases (RNRs) are key enzymes in DNA metabolism, with allosteric mechanisms controlling substrate specificity and overall activity. In RNRs, the activity master-switch, the ATP-cone, has been found exclusively in the catalytic subunit.
Inna Rozman Grinberg   +12 more
doaj   +1 more source

Cofactor Selectivity in Methylmalonyl Coenzyme A Mutase, a Model Cobamide-Dependent Enzyme. [PDF]

open access: yes, 2019
Cobamides, a uniquely diverse family of enzyme cofactors related to vitamin B12, are produced exclusively by bacteria and archaea but used in all domains of life.
Mok, Kenny   +5 more
core   +1 more source

3.3-Å resolution cryo-EM structure of human ribonucleotide reductase with substrate and allosteric regulators bound

open access: yeseLife, 2018
Ribonucleotide reductases (RNRs) convert ribonucleotides into deoxyribonucleotides, a reaction essential for DNA replication and repair. Human RNR requires two subunits for activity, the α subunit contains the active site, and the β subunit houses the ...
Edward J Brignole   +8 more
doaj   +1 more source

Home - About - Disclaimer - Privacy