Results 241 to 250 of about 509,011 (342)

Model‐Guided Systematic Metabolic Engineering for Enhanced Spinosad Biosynthesis in Saccharopolyspora spinosa NHF132

open access: yesAdvanced Science, EarlyView.
A genome‐scale model guided the systematic metabolic engineering of Saccharopolyspora spinosa, enabling a 5.5‐fold boost in the green bioinsecticide spinosad to 1.8 g L−1 and establishing an actinomycete framework for complex natural products overproduction.
Shuliu Wang   +9 more
wiley   +1 more source

The clinical impact of 16S ribosomal RNA PCR and sequencing in the identification of bacterial infections: a 7-year report from a Lebanese tertiary care center. [PDF]

open access: yesFront Cell Infect Microbiol
Youssef N   +14 more
europepmc   +1 more source

CRISPR‐DNA Polymerase Assisted Targeted Mutagenesis for Regulable Laboratory Evolution

open access: yesAdvanced Science, EarlyView.
This study develops an in vivo targeted mutagenesis system based on CRISPR‐guided bacteriophage T5 or T7 DNA polymerases. This system can enable all possible types of substitution mutations within an expanded mutagenesis window in Escherichia coli and exhibit application potential in different microorganisms, thus accelerating the evolution of ...
Shuaili Chen   +8 more
wiley   +1 more source

Unveiling Novel Viral Diversity, Biogeography, and Host Networks in Wildlife Through High‐Throughput Sequencing Data Mining

open access: yesAdvanced Science, EarlyView.
Analysis of 57 536 high‐throughput sequencing datasets uncovers a vast, hidden world of viruses in wildlife. The researchers reveal significant geographic and host‐specific patterns of viruses, and their surprising cross‐species transmissions, such as avian flu viruses infecting goats.
Hai Wang   +19 more
wiley   +1 more source

RPS27L Enhances Myogenesis and Muscle Mass by Targeting IGF1 Through Liquid‐Liquid Phase Separation

open access: yesAdvanced Science, EarlyView.
Muscle‐specific RPS27L knock‐in mice exhibit increased body weight, muscle mass, and enhanced muscle regenerative capacity. RPS27L drives myogenesis by promoting myoblast proliferation, while inhibiting myogenic differentiation. Mechanistically, RPS27L targets IGF1 protein by liquid‐liquid phase separation through its N‐terminal intrinsically ...
Xiaoqin Liu   +17 more
wiley   +1 more source

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