Results 121 to 130 of about 7,960 (219)

Abundance and functional diversity of riboswitches in microbial communities

BMC Genomics, 2007
Background Several recently completed large-scale enviromental sequencing projects produced a large amount of genetic information about microbial communities ('metagenomes') which is not biased towards cultured organisms.
Gelfand Mikhail S, Vitreschak Alexey G, Kazanov Marat D   +2 more
doaj   +1 more source

The riboswitch control of gene regulation. [PDF]

, 2012
(a) The two full length structures of the tenA thiamine pyrophosphate (TPP, blue oval) riboswitch are shown. Aptamer domain is highlighted in orange solid and broken lines. (b) Simplified diagram of riboswitch folding process for the tenA TPP riboswitch.
Tamar Schlick (11928), Giulio Quarta (183007), Ken Sin (183009)   +2 more
core   +1 more source

Mechanistic analysis of Riboswitch Ligand interactions provides insights into pharmacological control over gene expression

Nature Communications
Riboswitches are structured RNA elements that regulate gene expression upon binding to small molecule ligands. Understanding the mechanisms by which small molecules impact riboswitch activity is key to developing potent, selective ligands for these and ...
Shaifaly Parmar, Desta Doro Bume, Colleen M. Connelly, Robert E. Boer, Peri R. Prestwood, Zhen Wang, Henning Labuhn, Krishshanthi Sinnadurai, Adeline Feri, Jimmy Ouellet, Philip Homan, Tomoyuki Numata, John S. Schneekloth   +12 more
doaj   +1 more source

Scaffold-enabled high-resolution cryo-EM structure determination of RNA

Nature Communications
Cryo-EM structure determination of protein-free RNAs has remained difficult with most attempts yielding low to moderate resolution and lacking nucleotide-level detail.
Daniel B. Haack, Boris Rudolfs, Shouhong Jin, Alexandra Khitun, Kevin M. Weeks, Navtej Toor   +5 more
doaj   +1 more source

Characterization of the full-length btuB riboswitch from Klebsiella pneumoniae [PDF]

[eng] Riboswitches are cis-regulatory RNA elements on the mRNA level that control the expression of the downstream coding region. The interaction of the riboswitch with its specific metabolite, which is related to the function of the controlled gene ...
Musiari, Anastasia, Palou Mir, Joana, Barceló Oliver, Miquel, Sigel, Roland   +3 more
core   +1 more source

Investigation of the Binding Requirements of the $\textit{moaA}$ Riboswitch [PDF]

The molybdenum cofactor (Moco) riboswitch is a regulatory RNA element that modulates gene expression in response to Moco binding. We employed a combination of footprinting methods, including in-line probing and selective 2’-hydroxyl acylation analyzed by
Reichenbach, Maria
core   +1 more source

Docking-based Virtual Screening for the Discovery of RNA-targeting Molecules: Identification of Selective Riboswitch Binding Ligands [PDF]

We present herein one of the very first applications of molecular docking towards the discovery of novel RNA-targeting molecules. Our in-house docking program FITTED was used to perform independ-ent virtual screening campaigns against the TPP, SAM, and ...
Julia, Stille, Nicolas, Moitessier, Maira, Rivera, Joshua, Pottel, Anthony, Mittermaier, Maureen, McKeague, Nathania, Takyi, Omma, Ayon, Thershan, Satkunarajah   +8 more
core   +1 more source

Thiamin (Vitamin B1) Biosynthesis and Regulation: A Rich Source of Antimicrobial Drug Targets?

International Journal of Biological Sciences, 2011
Drug resistance of pathogens has necessitated the identification of novel targets for antibiotics. Thiamin (vitamin B1) is an essential cofactor for all organisms in its active form thiamin diphosphate (ThDP).
Qinglin Du, Honghai Wang, Jianping Xie
doaj  

High-throughput functional profiling and evolutionary covariation analysis of entire riboswitch sequences. [PDF]

Nucleic Acids Res
Hertz LM, Arce A, Rivas E, Lucks JB.
europepmc   +1 more source

Exploiting conformational changes in a caffeine aptamer to engineer synthetic RNA devices. [PDF]

Nucleic Acids Res
Kraus L, Gunawan VJ, Johannson R, Hallstein S, Block S, Campbell GA, Alameen A, Rosser SJ, Mol AA, Suess B.   +9 more
europepmc   +1 more source