Results 141 to 150 of about 2,869,940 (329)

A Unifying Scenario on the Origin and Evolution of Cellular and Viral Domains [PDF]

, 2009
The cellular theory on the nature of life has been one of the first major advancements in biology. Viruses, however, are the most abundant life forms, and their exclusion from mainstream biology and the Tree of Life (TOL) is a major paradox in biology ...
Claudiu I. Bandea
core   +1 more source

Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection [PDF]

, 2018
Nosocomial pathogens can cause life-threatening infections in neonates and immunocompromised patients. E. bugandensis (EB-247) is a recently described species of Enterobacter, associated with neonatal sepsis.
Bunk, Boyke, Chakraborty, Trinad, Doijad, Swapnil Prakash, Falgenhauer, Linda, Goesmann, Alexander, Gwozdzinski, Konrad, Hain, Torsten, Hegemann, Julian D., Imirzalioglu, Can, Jaiswal, Sangeeta, Mannala, Gopala Krishna, Marahiel, Mohamed A., Mraheil, Mobarak Abu, Mshana, Stephen E., Overmann, Jörg, Pati, Niladri Bhusan, Ryan, Daniel, Schultze, Tilman, Spröer, Cathrin, Suar, Mrutyunjay, Yao, Yancheng   +20 more
core   +2 more sources

Structural Basis of Transcription Initiation by Bacterial RNA Polymerase Holoenzyme*

Journal of Biological Chemistry, 2014
Background: Cellular RNA polymerases start transcription by de novo RNA priming. Results: Structures and biochemical studies of initially transcribing complexes elucidate the de novo transcription initiation and early stage of RNA transcription ...
R. Basu, B. Warner, V. Molodtsov, D. Pupov, D. Esyunina, C. Fernández‐Tornero, A. Kulbachinskiy, K. Murakami   +7 more
semanticscholar   +1 more source

Enhanced Sonodynamic Bacterial Elimination and Wound Healing Therapy Based on Lanthanide Ion Doped Bi2WO6 Nanosheets and Hydrogel Platform

Advanced Functional Materials, EarlyView.
Lanthanide ytterbium ions are introduced into Bi2WO6 nanosheets to boost ROS generation under US stimulation. These ytterbium‐doped Bi2WO6 nanosheets serve as efficient sonosensitizers, enabling broad‐spectrum bacterial eradication. To improve biocompatibility for in vivo sonodynamic therapy, the sonosensitizers are integrated into hydrogel matrix ...
Xinyue Lao, Qianqian Bai, Yifei Zhao, Xingyi Dai, Yuan Liu, Xiao Han, Jianhua Hao   +6 more
wiley   +1 more source

Nanomaterial‐Enhanced Biosensing: Mechanisms and Emerging Applications

Advanced Healthcare Materials, EarlyView.
Nanomaterial integration transforms biosensor capabilities through enhanced signal transduction, sensitivity, and selectivity. This review analyzes how nanoscale materials—from nanoparticles to nanosheets—leverage unique physicochemical properties to revolutionize electrochemical, optical, and electrical biosensing.
Younghak Cho, Yunyoung Choi, Yerim Jang, Hyejeong Seong   +3 more
wiley   +1 more source

An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity

eLife
In contrast to mammalian cells, bacterial cells lack mRNA polyadenylated tails, presenting a hurdle in isolating mRNA amidst the prevalent rRNA during single-cell RNA-seq. This study introduces a novel method, ribosomal RNA-derived cDNA depletion (RiboD),
Xiaodan Yan, Hebin Liao, Chenyi Wang, Chun Huang, Wei Zhang, Chunming Guo, Yingying Pu   +6 more
doaj   +1 more source

Optimized Rapid Amplification of cDNA Ends (RACE) for Mapping Bacterial mRNA Transcripts

BioTechniques, 2000
A simple, efficient and sensitive RACE-based procedure was developed for the determination of unknown 5′ regions from bacterial cDNA. A number of critical modifications were made to the standard RACE method, including the optimization of the RNA ...
Daniel Tillett, Brendan P. Burns, Brett A. Neilan   +2 more
doaj   +1 more source

Unprecedented High-Resolution View of Bacterial Operon Architecture Revealed by RNA Sequencing

mBio, 2014
We analyzed the transcriptome of Escherichia coli K-12 by strand-specific RNA sequencing at single-nucleotide resolution during steady-state (logarithmic-phase) growth and upon entry into stationary phase in glucose minimal medium.
T. Conway, J. Creecy, Scott M. Maddox, Joe E. Grissom, Trevor L. Conkle, Tyler M. Shadid, J. Teramoto, P. San Miguel, T. Shimada, A. Ishihama, H. Mori, B. Wanner   +11 more
semanticscholar   +1 more source

Secreted bacterial RNA: an unexplored avenue [PDF]

FEMS Microbiology Letters, 2018
Gradually, it is becoming clear that our well-being depends significantly on the contribution and composition of microorganisms that are associated with us. The majority of human-associated microorganisms are bacteria, which maintain their niche through interactions with the human host and neighboring microorganisms.
openaire   +2 more sources

Fluid Shear‐Controlled Pro/Anti‐Inflammatory Osteomodulatory Construct for Drug‐Free Immune Activation Through Cationic Ion Channel Activation

Advanced Healthcare Materials, EarlyView.
A 3D‐printed PCL scaffold coated with Arginine was laminated with electrospun polyvinyl alcohol (PVA) nanofibers containing cationic cellulose nanocrystals (PVA@cCNC). This created nanoisland‐like regions of aligned and random cCNC‐rich fibers. The composite scaffold, under fluid shear stimulation, modulated macrophage polarization from M1 to M2 ...
Keya Ganguly, Aayushi Randhawa, Sayan Deb Dutta, Jieun Lee, Youjin Seol, Mehran Mohammad Hossein Pour, Tejal V. Patil, Rumi Acharya, Ki‐Taek Lim   +8 more
wiley   +1 more source