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DragonRNA: Generality of DNA-primed RNA-extension activities by DNA-directed RNA polymerases [PDF]

open access: yesNucleic Acids Research
Abstract RNA polymerases (RNAPs) transcribe DNA into RNA. Several RNAPs, including from bacteriophages Sp6 and T7, Escherichia coli, and wheat germ, had been shown to add ribonucleotides to DNA 3′ ends. Mitochondria have their own RNAPs (mtRNAPs).
Emily Greenwald, Joon Park, Nimit Jain
exaly   +4 more sources

Metal A and metal B sites of nuclear RNA polymerases Pol IV and Pol V are required for siRNA-dependent DNA methylation and gene silencing. [PDF]

open access: yesPLoS ONE, 2009
Plants are unique among eukaryotes in having five multi-subunit nuclear RNA polymerases: the ubiquitous RNA polymerases I, II and III plus two plant-specific activities, nuclear RNA polymerases IV and V (previously known as Polymerases IVa and IVb).
Jeremy R Haag   +2 more
doaj   +3 more sources

A Pol V-mediated silencing, independent of RNA-directed DNA methylation, applies to 5S rDNA. [PDF]

open access: yesPLoS Genetics, 2009
The plant-specific RNA polymerases Pol IV and Pol V are essential to RNA-directed DNA methylation (RdDM), which also requires activities from RDR2 (RNA-Dependent RNA Polymerase 2), DCL3 (Dicer-Like 3), AGO4 (Argonaute), and DRM2 (Domains Rearranged ...
Julien Douet   +2 more
doaj   +1 more source

DNA topoisomerase 1α promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis. [PDF]

open access: yesPLoS Genetics, 2014
RNA-directed DNA methylation (RdDM) and histone H3 lysine 9 dimethylation (H3K9me2) are related transcriptional silencing mechanisms that target transposable elements (TEs) and repeats to maintain genome stability in plants. RdDM is mediated by small and
Thanh Theresa Dinh   +14 more
doaj   +1 more source

Taking the Wheel – de novo DNA Methylation as a Driving Force of Plant Embryonic Development

open access: yesFrontiers in Plant Science, 2021
During plant embryogenesis, regardless of whether it begins with a fertilized egg cell (zygotic embryogenesis) or an induced somatic cell (somatic embryogenesis), significant epigenetic reprogramming occurs with the purpose of parental or vegetative ...
Lucija Markulin   +6 more
doaj   +1 more source

Understanding the Evolution of Mitochondrial Genomes in the Green Macroalgal Genus Ulva (Ulvophyceae, Chlorophyta)

open access: yesFrontiers in Marine Science, 2022
To gain more insights into the evolution of mitochondrial genomes (mitogenomes or mtDNAs) in the green macroalgal genus Ulva (Ulvophyceae, Chlorophyta), we sequenced seven Ulva mitogenomes from six species as well as one Percursaria mitogenome as ...
Feng Liu   +10 more
doaj   +1 more source

Arabidopsis MED18 Interaction With RNA Pol IV and V Subunit NRPD2a in Transcriptional Regulation of Plant Immune Responses

open access: yesFrontiers in Plant Science, 2021
Mediator is a conserved multiprotein complex important for transcription by RNA polymerase II (Pol II). Arabidopsis Mediator subunit MED18 regulates flowering, hormone signaling and plant immunity.
Yan Zhang   +6 more
doaj   +1 more source

AGO6 functions in RNA-mediated transcriptional gene silencing in shoot and root meristems in Arabidopsis thaliana. [PDF]

open access: yesPLoS ONE, 2011
RNA-directed DNA methylation (RdDM) is a small interfering RNA (siRNA)-mediated epigenetic modification that contributes to transposon silencing in plants. RdDM requires a complex transcriptional machinery that includes specialized RNA polymerases, named
Changho Eun   +8 more
doaj   +1 more source

Essential Oils Prime Epigenetic and Metabolomic Changes in Tomato Defense Against Fusarium oxysporum

open access: yesFrontiers in Plant Science, 2022
In this work, we studied the direct and indirect plant protection effects of an Artemisia absinthium essential oil (AEO) on tomato seedlings against Fusarium oxysporum sp. oxysporum radicis lycopersici (Fol). AEO exhibited a toxic effect in vitro against
Serine Soudani   +5 more
doaj   +1 more source

A Bayesian approach to analyse genetic variation within RNA viral populations. [PDF]

open access: yesPLoS Computational Biology, 2011
The development of modern and affordable sequencing technologies has allowed the study of viral populations to an unprecedented depth. This is of particular interest for the study of within-host RNA viral populations, where variation due to error-prone ...
Trevelyan J McKinley   +4 more
doaj   +1 more source

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