Results 11 to 20 of about 26,180 (234)

AGO6 functions in RNA-mediated transcriptional gene silencing in shoot and root meristems in Arabidopsis thaliana. [PDF]

open access: yesPLoS ONE, 2011
RNA-directed DNA methylation (RdDM) is a small interfering RNA (siRNA)-mediated epigenetic modification that contributes to transposon silencing in plants. RdDM requires a complex transcriptional machinery that includes specialized RNA polymerases, named
Changho Eun   +8 more
doaj   +1 more source

Essential Oils Prime Epigenetic and Metabolomic Changes in Tomato Defense Against Fusarium oxysporum

open access: yesFrontiers in Plant Science, 2022
In this work, we studied the direct and indirect plant protection effects of an Artemisia absinthium essential oil (AEO) on tomato seedlings against Fusarium oxysporum sp. oxysporum radicis lycopersici (Fol). AEO exhibited a toxic effect in vitro against
Serine Soudani   +5 more
doaj   +1 more source

A Bayesian approach to analyse genetic variation within RNA viral populations. [PDF]

open access: yesPLoS Computational Biology, 2011
The development of modern and affordable sequencing technologies has allowed the study of viral populations to an unprecedented depth. This is of particular interest for the study of within-host RNA viral populations, where variation due to error-prone ...
Trevelyan J McKinley   +4 more
doaj   +1 more source

Functional Consequences of Subunit Diversity in RNA Polymerases II and V

open access: yesCell Reports, 2012
Multisubunit RNA polymerases IV and V (Pol IV and Pol V) evolved as specialized forms of Pol II that mediate RNA-directed DNA methylation (RdDM) and transcriptional silencing of transposons, viruses, and endogenous repeats in plants.
Ek Han Tan   +3 more
doaj   +1 more source

Use of Site-Specifically Tethered Chemical Nucleases to Study Macromolecular Reactions

open access: yesBiological Procedures Online, 2003
During a complex macromolecular reaction multiple changes in molecular conformation and interactions with ligands may occur. X-ray crystallography may provide only a limited set of snapshots of these changes.
Mukherjee Srabani, Sousa Rui
doaj   +1 more source

The RNA silencing enzyme RNA polymerase v is required for plant immunity. [PDF]

open access: yesPLoS Genetics, 2011
RNA-directed DNA methylation (RdDM) is an epigenetic control mechanism driven by small interfering RNAs (siRNAs) that influence gene function. In plants, little is known of the involvement of the RdDM pathway in regulating traits related to immune ...
Ana López   +4 more
doaj   +1 more source

RNase-like domain in DNA-directed RNA polymerase II. [PDF]

open access: yesProceedings of the National Academy of Sciences, 1991
DNA-directed RNA polymerase is responsible for gene expression. Despite its importance, many details of its function and higher-order structure still remain unknown. We report here a local sequence similarity between the second largest subunit of RNA polymerase II and bacterial RNases Ba (barnase), Bi, and St. The most remarkable similarity is that the
T, Shirai, M, Go
openaire   +2 more sources

Primer Extension Reactions for the PCR- based α- complementation Assay

open access: yesBio-Protocol, 2015
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity of the
Vasudevan Achuthan, Jeffrey DeStefano
doaj   +1 more source

Functional Diversification of Maize RNA Polymerase IV and V Subtypes via Alternative Catalytic Subunits

open access: yesCell Reports, 2014
Summary: Unlike nuclear multisubunit RNA polymerases I, II, and III, whose subunit compositions are conserved throughout eukaryotes, plant RNA polymerases IV and V are nonessential, Pol II-related enzymes whose subunit compositions are still evolving ...
Jeremy R. Haag   +13 more
doaj   +1 more source

In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase

open access: yesAnimals, 2023
The genus Capripoxvirus belongs to the Poxviridae family. The sheeppox, goatpox, and lumpy skin disease viruses are three species of this genus with 96% identity in their genomes. These are financially devastating viral infections among cattle, which cause a reduction in animal products and lead to a loss in livestock industries.
Ghulam Mustafa   +6 more
openaire   +3 more sources

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