Solid phase chemistry to covalently and reversibly capture thiolated RNA. [PDF]
, 2018 Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s4U) metabolic labeling and solid phase chemistry.
Canzio, Daniele +3 more
core +1 more source
Genome-wide control of RNA polymerase II activity by cohesin.
PLoS Genetics, 2013 Cohesin is a well-known mediator of sister chromatid cohesion, but it also influences gene expression and development. These non-canonical roles of cohesin are not well understood, but are vital: gene expression and development are altered by modest ...
Cheri A Schaaf +8 more
doaj +1 more source
High-Resolution Phenotypic Landscape of the RNA Polymerase II Trigger Loop. [PDF]
PLoS Genetics, 2016 The active sites of multisubunit RNA polymerases have a "trigger loop" (TL) that multitasks in substrate selection, catalysis, and translocation.
Chenxi Qiu +13 more
doaj +1 more source
Life, 2022 RNAs not only offer valuable information regarding our bodies but also regulate cellular functions, allowing for their specific manipulations to be extensively explored for many different biological and clinical applications.
Hyesung Jo, Jiyun Beon, Seung Soo Oh
doaj +1 more source
Combinatorial Recruitment of CREB, C/EBPβ and c-Jun Determines Activation of Promoters upon Keratinocyte Differentiation [PDF]
, 2013 Background: Transcription factors CREB, C/EBPβ and Jun regulate genes involved in keratinocyte proliferation and differentiation. We questioned if specific combinations of CREB, C/EBPβ and c-Jun bound to promoters correlate with RNA polymerase II binding,
Bhattacharya, Paramita +4 more
core +3 more sources
Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation.
PLoS ONE, 2008 The production of a functional mRNA is regulated at every step of transcription. An area not well-understood is the transition of RNA polymerase II from elongation to termination. The S.
Jessica L Dermody +9 more
doaj +1 more source
The RNA Polymerase II Core Promoter in Drosophila. [PDF]
, 2019 Transcription by RNA polymerase II initiates at the core promoter, which is sometimes referred to as the "gateway to transcription." Here, we describe the properties of the RNA polymerase II core promoter in Drosophila The core promoter is at a strategic
Kadonaga, James T +2 more
core +1 more source
High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier. [PDF]
, 2019 Nucleosomes represent mechanical and energetic barriers that RNA Polymerase II (Pol II) must overcome during transcription. A high-resolution description of the barrier topography, its modulation by epigenetic modifications, and their effects on Pol II ...
Brown, Aidan I +9 more
core +2 more sources
PIP2-Effector Protein MPRIP Regulates RNA Polymerase II Condensation and Transcription
Biomolecules, 2023 The specific post-translational modifications of the C-terminal domain (CTD) of the Rpb1 subunit of RNA polymerase II (RNAPII) correlate with different stages of transcription.
Can Balaban +5 more
doaj +1 more source
Writing a wrong: Coupled RNA polymerase II transcription and RNA quality control [PDF]
, 2019 Processing and maturation of precursor RNA species is coupled to RNA polymerase II transcription. Co-transcriptional RNA processing helps to ensure efficient and proper capping, splicing, and 3' end processing of different RNA species to help ensure ...
Hughes, Katlyn D. +3 more
core +1 more source