Results 41 to 50 of about 535,527 (262)

PIP2-Effector Protein MPRIP Regulates RNA Polymerase II Condensation and Transcription

Biomolecules, 2023
The specific post-translational modifications of the C-terminal domain (CTD) of the Rpb1 subunit of RNA polymerase II (RNAPII) correlate with different stages of transcription.
Can Balaban, Martin Sztacho, Ludovica Antiga, Ana Miladinović, Masahiko Harata, Pavel Hozák   +5 more
doaj   +1 more source

Genome-wide control of RNA polymerase II activity by cohesin.

PLoS Genetics, 2013
Cohesin is a well-known mediator of sister chromatid cohesion, but it also influences gene expression and development. These non-canonical roles of cohesin are not well understood, but are vital: gene expression and development are altered by modest ...
Cheri A Schaaf, Hojoong Kwak, Amanda Koenig, Ziva Misulovin, David W Gohara, Audrey Watson, Yanjiao Zhou, John T Lis, Dale Dorsett   +8 more
doaj   +1 more source

RNA Polymerase II CTD phosphatase Rtr1 fine-tunes transcription termination.

PLoS Genetics, 2020
RNA Polymerase II (RNAPII) transcription termination is regulated by the phosphorylation status of the C-terminal domain (CTD). The phosphatase Rtr1 has been shown to regulate serine 5 phosphorylation on the CTD; however, its role in the regulation of ...
Jose F Victorino, Melanie J Fox, Whitney R Smith-Kinnaman, Sarah A Peck Justice, Katlyn H Burriss, Asha K Boyd, Megan A Zimmerly, Rachel R Chan, Gerald O Hunter, Yunlong Liu, Amber L Mosley   +10 more
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Modulation of RNA primer formation by Mn(II)-substituted T7 DNA primase

Scientific Reports, 2017
Lagging strand DNA synthesis by DNA polymerase requires RNA primers produced by DNA primase. The N-terminal primase domain of the gene 4 protein of phage T7 comprises a zinc-binding domain that recognizes a specific DNA sequence and an RNA polymerase ...
Stefan Ilic, Sabine R. Akabayov, Roy Froimovici, Ron Meiry, Dan Vilenchik, Alfredo Hernandez, Haribabu Arthanari, Barak Akabayov   +7 more
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Promoter clearance by RNA polymerase II [PDF]

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2013
Many changes must occur to the RNA polymerase II (pol II) transcription complex as it makes the transition from initiation into transcript elongation. During this intermediate phase of transcription, contact with initiation factors is lost and stable association with the nascent transcript is established.
openaire   +2 more sources

Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation.

PLoS ONE, 2008
The production of a functional mRNA is regulated at every step of transcription. An area not well-understood is the transition of RNA polymerase II from elongation to termination. The S.
Jessica L Dermody, Jonathan M Dreyfuss, Judit Villén, Babatunde Ogundipe, Steven P Gygi, Peter J Park, Alfred S Ponticelli, Claire L Moore, Stephen Buratowski, Miriam E Bucheli   +9 more
doaj   +1 more source

Repression of RNA polymerase II transcription by a Drosophila oligopeptide. [PDF]

PLoS ONE, 2008
BACKGROUND: Germline progenitors resist signals that promote differentiation into somatic cells. This occurs through the transient repression in primordial germ cells of RNA polymerase II, specifically by disrupting Ser2 phosphorylation on its C-terminal
Gyula Timinszky, Miriam Bortfeld, Andreas G Ladurner   +2 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source