Results 21 to 30 of about 3,029,744 (275)

Primer Extension Reactions for the PCR- based α- complementation Assay

Bio-Protocol, 2015
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity of the
Vasudevan Achuthan, Jeffrey DeStefano
doaj   +1 more source

Functional Diversification of Maize RNA Polymerase IV and V Subtypes via Alternative Catalytic Subunits

Cell Reports, 2014
Summary: Unlike nuclear multisubunit RNA polymerases I, II, and III, whose subunit compositions are conserved throughout eukaryotes, plant RNA polymerases IV and V are nonessential, Pol II-related enzymes whose subunit compositions are still evolving ...
Jeremy R. Haag, Brent Brower-Toland, Elysia K. Krieger, Lyudmila Sidorenko, Carrie D. Nicora, Angela D. Norbeck, Andre Irsigler, Huachun LaRue, Jan Brzeski, Karen McGinnis, Sergey Ivashuta, Ljiljana Pasa-Tolic, Vicki L. Chandler, Craig S. Pikaard   +13 more
doaj   +1 more source

In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase

Animals, 2023
The genus Capripoxvirus belongs to the Poxviridae family. The sheeppox, goatpox, and lumpy skin disease viruses are three species of this genus with 96% identity in their genomes. These are financially devastating viral infections among cattle, which cause a reduction in animal products and lead to a loss in livestock industries.
Ghulam Mustafa, Hafiza Salaha Mahrosh, Mahwish Salman, Muhammad Ali, Rawaba Arif, Sibtain Ahmed, Hossam Ebaid   +6 more
openaire   +3 more sources

Inhibition of Ebola Virus by Anti-Ebola miRNAs in silico

Journal of Infection in Developing Countries, 2016
Introduction: MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate transcriptional and posttranscriptional gene regulation of the organisms. miRNA provides immune defense when the body is faced with challenges intracellular agents.
Zhabiz Golkar, Roshan Battaria, Donald G Pace, Omar Bagasra   +3 more
doaj   +1 more source

Isolation and physical properties of the DNA‐directed RNA polymerase from Thermus thermophilus HB8 [PDF]

European Journal of Biochemistry, 1990
The DNA‐directed RNA polymerase from the extremely thermophilic eubacterium Thermus thermophilus HB8 was purified employing a new and rapid method. The subunit pattern of the enzyme, analyzed by SDS gel electrophoresis, was interpreted as: 140 kDa and 170 kDa for β and β′, 40 kDa for α and 92 kDa for ó.
S, Wnendt, R K, Hartmann, N, Ulbrich, V A, Erdmann   +3 more
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Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians [PDF]

Immunity & Ageing, 2016
Recently, phage display technology has made it possible to define the circulating repertoire of humoral immunity. This study was designed to define the circulating antibodies specific to centenarians.We used a phage-displayed combinatorial peptide library to screen for peptides (YSATLRY and YSPTLFY) that preferentially react with the IgG fraction of ...
Han, Jungwon, Park, So-Hyun, Kim, Dong-Jo, Kim, Hyori, Choi, Yoon-Ho, Lee, Jong-Hyuk, Park, Sang Chul, Chung, Junho   +7 more
openaire   +3 more sources

Investigation of Multi-Subunit Mycobacterium tuberculosis DNA-Directed RNA Polymerase and Its Rifampicin Resistant Mutants

International Journal of Molecular Sciences, 2023
Emerging Mycobacterium tuberculosis (Mtb) resistant strains have continued to limit the efficacies of existing antitubercular therapies. More specifically, mutations in the RNA replicative machinery of Mtb, RNA polymerase (RNAP), have been widely linked to rifampicin (RIF) resistance, which has led to therapeutic failures in many clinical cases ...
Mokgerwa Zacharia Monama, Fisayo Olotu, Özlem Tastan Bishop   +2 more
openaire   +2 more sources

Topoisomerase IIα promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation [PDF]

, 2013
Type II DNA topoisomerases catalyse DNA double-strand cleavage, passage and re-ligation to effect topological changes. There is considerable interest in elucidating topoisomerase II roles, particularly as these proteins are targets for anti-cancer drugs.
Porter, Andrew C. G., Panov, Konstantin I; id_orcid, Zomerdijk, Joost C.B.M., Russell, J., Miller, G. (Gail), Zomerdijk, Joost C B M, Porter, A.C., Porter, Andrew C G, Volkov, A., Panov, K.I., Panov, Konstantin I., Russell, J. (Jackie), Panova, T., Zomerdijk, J.C., Miller, Gail, Porter, Andrew C.G., Miller, G., Panova, Tatiana, Russell, Jackie, Ray, S., Ray, Swagat, Volkov, Arsen, Zomerdijk, Joost C. B. M.   +22 more
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Entropy-driven genome organization [PDF]

, 2006
DNA and RNA polymerases active on bacterial and human genomes in the crowded environment of a cell are modeled as beads spaced along a string. Aggregation of the large polymerizing complexes increases the entropy of the system through an increase in ...
Davide Marenduzzo, Cook, P. R., Micheletti, Cristian, Cristian Micheletti, Micheletti, C. (Cristian), Marenduzzo, Davide, Peter R. Cookz, COOK P. R., Peter R. Cook, MARENDUZZO D, Cook PR, Micheletti C   +11 more
core   +1 more source

DNA-Directed DNA Polymerases Evolve From Reverse Transcriptase [PDF]

, 2013
Scientists have been debating for decades the origin of life on earth. A number of hypotheses were proposed as to what emerged first RNA or DNA; with most scientists are in favour of the "RNA World" hypothesis. Assuming RNA emerged first, it fellow that
Zhang, Lei
core   +1 more source