Results 271 to 280 of about 819,809 (314)
Some of the next articles are maybe not open access.
Cold Spring Harbor Protocols, 2012
In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
openaire +2 more sources
In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
openaire +2 more sources
Current Opinion in Structural Biology, 2001
The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
openaire +2 more sources
The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
openaire +2 more sources
Current Protocols in Molecular Biology, 1993
AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
K J, Reddy, M, Gilman
openaire +2 more sources
AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
K J, Reddy, M, Gilman
openaire +2 more sources
Bacterial persistence by RNA endonucleases.
Proceedings of the National Academy of Sciences of the United States of America, 2011Bacteria form persisters, individual cells that are highly tolerant to different types of antibiotics. Persister cells are genetically identical to nontolerant kin but have entered a dormant state in which they are recalcitrant to the killing activity of the antibiotics. The molecular mechanisms underlying bacterial persistence are unknown.
Maisonneuve, E +3 more
openaire +3 more sources
Bacterial growth physiology and RNA metabolism
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2020Bacteria are sophisticated systems with high capacity and flexibility to adapt to various environmental conditions. Each prokaryote however possesses a defined metabolic network, which sets its overall metabolic capacity, and therefore the maximal growth rate that can be reached. To achieve optimal growth, bacteria adopt various molecular strategies to
Planson, Anne-Gaëlle +3 more
openaire +3 more sources
35S incorporation into bacterial RNA
Biochemical and Biophysical Research Communications, 1971Abstract When Escherichia Coli MRE600 was grown in the presence of Na 2 35 SO 4 as the only source of sulfur in the medium, 35 S was incorporated into 4S RNA. 35 S incorporation could not be detected in the ribosomal RNA species: 5S, 16S and 23S.
openaire +2 more sources
Defining Bacterial RNA-RNA Interactomes Using CLASH
Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial pathogen accounting for high mortality rates among infected patients. Transcriptomic regulation by small RNAs (sRNAs) has been shown to regulate networks promoting antibiotic resistance and virulence in S. aureus.Sofia, Esteban-Serna +4 more
openaire +2 more sources
Bacterial ribosomal RNA in pieces
Molecular Microbiology, 2005SummaryThe exact knowledge on the ribosomal RNA (rRNA) structure is an important prerequisite for work with rRNA sequences in bioinformatic analyses and in experimental research. Most available rRNA sequences of bacteria are based on gene sequences and on similarity analyses using Escherichia coli rRNA as a standard.
openaire +2 more sources
Identification of Bacterial Small RNAs by RNA Sequencing
2014Small regulatory RNAs (sRNAs) in bacteria are known to modulate gene expression and control a variety of processes including metabolic reactions, stress responses, and pathogenesis in response to environmental signals. A method to identify bacterial sRNAs on a genome-wide scale based on RNA sequencing (RNA-seq) is described that involves the ...
María, Gómez-Lozano +3 more
openaire +2 more sources
RNA Thermosensors in Bacterial Pathogens
2009During the course of an infection, a pathogenic bacterium has to sense the environment and adjust its gene expression appropriately. One such environmental cue is the difference in temperature inside and outside the host. RNA thermosensors are structures that can respond to differences in temperature by altering their conformation and thereby allowing ...
openaire +2 more sources