Results 271 to 280 of about 532,169 (312)
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Current Protocols in Molecular Biology, 1993
AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
K J, Reddy, M, Gilman
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AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
K J, Reddy, M, Gilman
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On the biosynthesis of bacterial ribosomal RNA
Molecular Biology Reports, 1973By comparison of the fingerprints of 5S and 23S ribosomal RNAs from Bacillus licheniformis with that of the precursor of 23S ribosomal RNA, it can be shown that 5S RNA is not a part of the precursor of 23S ribosomal RNA.
T J, Stoof, R J, Planta
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Cold Spring Harbor Protocols, 2012
In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
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In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
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Current Opinion in Structural Biology, 2001
The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
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The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
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Bacterial persistence by RNA endonucleases.
Proceedings of the National Academy of Sciences of the United States of America, 2011Bacteria form persisters, individual cells that are highly tolerant to different types of antibiotics. Persister cells are genetically identical to nontolerant kin but have entered a dormant state in which they are recalcitrant to the killing activity of the antibiotics. The molecular mechanisms underlying bacterial persistence are unknown.
Maisonneuve, E +3 more
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The Decay of Bacterial Messenger RNA
1996Publisher Summary The many demonstrations that the Escherichia coli (E. coli ) rne gene product (RNase E) is involved in messenger RNA (mRNA) decay have given real impetus to the study of this unusual protein's properties and role. The recent attention given to the polyadenylylation of bacterial mRNAs and the discovery that polyadenylylation ...
D P, Nierlich, G J, Murakawa
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Bacterial ribosomal RNA in pieces
Molecular Microbiology, 2005SummaryThe exact knowledge on the ribosomal RNA (rRNA) structure is an important prerequisite for work with rRNA sequences in bioinformatic analyses and in experimental research. Most available rRNA sequences of bacteria are based on gene sequences and on similarity analyses using Escherichia coli rRNA as a standard.
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Identification of Bacterial Small RNAs by RNA Sequencing
2014Small regulatory RNAs (sRNAs) in bacteria are known to modulate gene expression and control a variety of processes including metabolic reactions, stress responses, and pathogenesis in response to environmental signals. A method to identify bacterial sRNAs on a genome-wide scale based on RNA sequencing (RNA-seq) is described that involves the ...
María, Gómez-Lozano +3 more
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Transfer of the immunization to a bacterial antigen by RNA
Experientia, 1966L'iniezione ad animali normali di ARN estratto dalla milza di animali immunizzati con antigene ‘H’ diS. typhi, provoca la comparsa, nel siero degli animali riceventi, di anticorpi diretti contro tale antigene.
S, Esposito, E L, Lignière
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35S incorporation into bacterial RNA
Biochemical and Biophysical Research Communications, 1971Abstract When Escherichia Coli MRE600 was grown in the presence of Na 2 35 SO 4 as the only source of sulfur in the medium, 35 S was incorporated into 4S RNA. 35 S incorporation could not be detected in the ribosomal RNA species: 5S, 16S and 23S.
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