Results 261 to 270 of about 388,782 (280)

The relationship between RNA catalytic processes

Origins of life and evolution of the biosphere, 1988
Proposals that an RNA-based genetic system preceded DNA, stem from the ability of RNA to store genetic information and to promote simple catalysis. However, to be a valid basis for the RNA world, RNA catalysis must demonstrate or be related to intrinsic chemical properties which could have existed in primordial times.
Denis Gravel, B. Franz Lang, Robert Cedergren   +2 more
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Transition State Stabilization by a Catalytic RNA

Science, 2002
The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate–hairpin ribozyme complex with structures of precursor ...
Adrian R. Ferré-D'Amaré, Snorri Th. Sigurdsson, Archna P. Massey, Peter B. Rupert   +3 more
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The Catalytic Mechanism of DNA and RNA Polymerases

ACS Catalysis, 2018
DNA and RNA polymerases (Pols) catalyze nucleic acid biosynthesis in all domains of life, with implications for human diseases and health. Pols carry out nucleic acid extension through the addition of one incoming nucleotide trisphosphate at the 3′-OH terminus of the growing primer strand, at every catalytic cycle. Thus, Pol catalysis involves chemical
Vito Genna, Elisa Donati, Marco De Vivo
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In Vitro Selection of Catalytic RNA

1996
After the first examples of catalytic RNAs raised to prominence earlier proposals that RNA was a likely protagonist in the dawn of evolution, molecular biologists began to ask in earnest about the range of possible RNA activities. This question provided much of the impetus for the development of in vitro RNA selection (Ellington and Szostak 1990; Green
Kelly P. Williams, David P. Bartel
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Structure‐Selective Catalytic Alkylation of DNA and RNA

Angewandte Chemie, 2012
A variety of nucleic acids can be catalytically alkylated with rhodium-carbenoids generated from diazo compounds in aqueous buffer through an NH insertion process (see scheme; MES=2-(N-morpholino)ethanesulfonic acid). The method specifically targets unpaired bases such as those present in single strands, turn regions, and overhangs while leaving double-
Tishinov, Kiril, Schmidt, Kristina, Häussinger, Daniel, Gillingham, Dennis   +3 more
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The efficiency and versatility of catalytic RNA: implications for an RNA world

Gene, 1993
Because RNA is chemically and structurally dissimilar from protein, the finding of catalytic activity in RNA was initially surprising. Quantitative measurements of reaction rates show that RNA can be as efficient a catalyst as protein. On the other hand, the potential versatility of RNA to catalyze diverse types of reactions has only begun to be ...
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Antisense Catalytic RNAs as Therapeutic Agents

1994
Publisher Summary This chapter presents an overview of well-characterized ribozymes with an emphasis on the problems that remain to be overcome before ribozyme therapy can be made a useful form of medical treatment. The chapter illustrates that many techniques are currently being used to enhance ribozyme activity.
Daniela Castanotto, John J. Rossi, Nava Sarver   +2 more
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Amplification, mutation and selection of catalytic RNA

Gene, 1989
RNA, by virtue of its genotypic and phenotypic properties, is a suitable substrate for molecular evolution in the laboratory. We have developed techniques for the rapid amplification, mutation and selection of catalytic RNA. By combining these techniques in an iterative fashion, we are attempting to construct an RNA-based evolving system. Such a system
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Catalytic DNA and RNA for Targeting MDR1 mRNA

Nucleosides, Nucleotides and Nucleic Acids, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
Valentin V. Vlassov, Mark Lukin, Alya G. Venyaminova, M. Repkova, A. A. Fokina, M. A. Kuznetsova   +5 more
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Catalytic metallodrugs targeting HCV IRES RNA

Chemical Communications, 2012
A catalytic metallodrug that targets stem-loop IIb of the internal ribosomal entry site (IRES) RNA of hepatitis C virus (HCV) demonstrates enzyme-like turnover with K(M) of 0.85 μM, k(cat) of 0.53 min(-1), and a turnover number of 31.9 for Cu·GGHYrFK-amide (1-Cu), and yielded an antiviral activity (IC(50)) of 0.58 μM in an HCV cellular replicon assay.
James A. Cowan, Seth Bradford
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