Results 91 to 100 of about 123,859 (242)

Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea

Scientific Reports, 2018
Sclerotinia sclerotiorum, the causal agent of white stem rot, is responsible for significant losses in crop yields around the globe. While our understanding of S.
Austein G. McLoughlin, Nick Wytinck, Philip L. Walker, Ian J. Girard, Khalid Y. Rashid, Teresa de Kievit, W. G. Dilantha Fernando, Steve Whyard, Mark F. Belmonte   +8 more
doaj   +1 more source

LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

BMC Research Notes, 2012
Background Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic
Yaffe Hila, Buxdorf Kobi, Shapira Illil, Ein-Gedi Shachaf, Moyal-Ben Zvi Michal, Fridman Eyal, Moshelion Menachem, Levy Maggie   +7 more
doaj   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Comparative Analysis of Fungal Communities and the Key “Golden‐Flower” Fungi in Fu Brick Tea From Seven Chinese Regions and Their Effects on the Quality of Summer‐Autumn Tea

Food Frontiers, EarlyView.
This study systematically investigated 52 Fu brick tea (FBT) samples collected from seven representative regions in China. High‐through put sequencing revealed that Aspergillus, Wallemia, and Candida were the predominant fungal genera across the samples.
Lisha Ran, Jinqi Zhao, Mengyao Ma, Yiqiao Zhao, Shiyu Guo, Yu Xiao, Zhonghua Liu, Jianan Huang, Kunbo Wang, Mingzhi Zhu   +9 more
wiley   +1 more source

The endocytic pathway for absorption of exogenous RNAs in Verticillium dahliae

mLife
RNAi technologies have been exploited to control viruses, pests, oomycetes, and fungal phytopathogens that cause disasters in host plants, including many agronomically significant crops.
Chuanhui Liu, Chen Cui, Guanyin Zhou, Feng Gao, Jianhua Zhao, Huishan Guo, Yun Jin   +6 more
doaj   +1 more source

An Improved Transformation System for Molecular Biology Research in Peach Fruit

Future Postharvest and Food, EarlyView.
Schematic Diagram of Repeated Agrobacterium‐Mediated Transformation. A triple‐stage on‐tree Agrobacterium injection enables gene manipulation in peach with up to 32‐day expression, supporting efficient, nonheritable functional analysis without tissue culture.
Chunhong Li, Yijia Xia, Minghua Zhou, Fei Xiang, Wancheng Huang, Xuansheng Tan, Yanyu Zou, Kaituo Wang   +7 more
wiley   +1 more source

Dothistroma septosporum and Dothistroma pini, the causal agents of Dothistroma needle blight, are infected by multiple viruses

Virus Research
Dothistroma septosporum and Dothistroma pini are severe foliar pathogens of conifers. They infect a broad spectrum of hosts (mainly Pinus spp.), causing chlorosis, defoliation of needles, and eventually the death of pine trees in extreme cases ...
Miloš Trifković, Ondřej Hejna, Anna Kuznetsova, Martin Mullett, Libor Jankovský, Leticia Botella   +5 more
doaj   +1 more source

Plant‐derived extracellular vesicle‐like particles as novel osteoanabolic agents: Activating bone morphogenetic protein 2/Smads signaling axis for bone regeneration in postmenopausal osteoporosis

Interdisciplinary Medicine, EarlyView.
Illustration depicting the isolation of Dipsaci Radix derived extracellular vesicle‐like particles and their function in alleviating osteoporosis. (A) Extraction and characterization detection process of extracellular vesicle‐like particles derived from Dipsaci Radix roots. (B) Cell experiments in vitro, Dipsaci Radix derived extracellular vesicle‐like
Qing Zhao, Yue Cao, Zhengting Wu, Yingqi Cao, Qi You, Yuanyuan Xia, Xuejun Tan, Dongxiao Li, Tianxin Qiu, Xiuping Cai, Zhaodi Guo, Lei Zheng, Kewei Zhao   +12 more
wiley   +1 more source

The Application Value of Bronchoalveolar Lavage Fluid Metagenomic Next‐Generation Sequencing in Moderate‐to‐Severe Bronchiectasis

Journal of Clinical Laboratory Analysis, EarlyView.
This study analyzed the clinical data of 52 patients with moderate‐to‐severe bronchiectasis who were admitted to our hospital. It explored the diagnostic value of bronchoalveolar lavage fluid (BALF) metagenomic next‐generation sequencing (mNGS) in identifying infectious pathogens in patients with moderate‐to‐severe bronchiectasis, and clarified the ...
Jiachun Li, Qiuping Quan, Junshan Chen, Xiaoyun Jian, Weijie Zhan, Jingmin Wang, Rongbin Jiang   +6 more
wiley   +1 more source

RNAi technology: A Novel approaches against fungal infections

Research in Molecular Medicine, 2014
Despite the introduction of new antifungal agents, resistances to antifungal therapy continue to increase and outcome of invasive fungal infections treatment is frequently suboptimal.
Maryam Moazeni, Mojtaba Nabili, Hamid Badali, Mahdi Abastabar   +3 more
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