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RNA interference: roles in fungal biology
Current Opinion in Microbiology, 2008The discovery of RNA interference (RNAi) has been the major recent breakthrough in biology. Only a few years after its discovery, RNAi has rapidly become a powerful reverse genetic tool, especially in organisms where gene targeting is inefficient and/or time-consuming.
Hitoshi, Nakayashiki, Quoc Bao, Nguyen
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RNA-based sensitive fungal pathogen detection
2023AbstractDetecting fungal pathogens, a major cause of severe systemic infections, remains challenging due to the difficulty and time-consuming nature of diagnostic methods. This delay in identification hinders targeted treatment decisions and may lead to unnecessary use of broad-spectrum antibiotics.
Julia Micheel +6 more
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RNA Interference in Plant Protection from Fungal and Oomycet Infection
Прикладная биохимия и микробиология, 2022Phytopathogenic fungi pose a threat to food security, limiting the biological potential of agricultural crops and reducing the quality of products. New plant protection methods based on natural systemic and cellular phytoimmunity are being developed to date, where a unique mechanism, described by the term “RNA interference” (RNAi), occupies a special ...
I. V. Maksimov +2 more
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Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control
2022Trans-kingdom RNA interference (RNAi) has been reported in several plant-fungal pathosystems. Our recent works have demonstrated natural RNAi transmission from cotton plants into Verticillium dahliae, a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues, and successful application of trans-kingdom RNAi in ...
Tao, Zhang +3 more
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Heterogeneity of 5 S RNA in Fungal Ribosomes
Science, 1985Neurospora crassa has at least seven types of 5 S RNA genes (α, β, γ, ε, δ, ζ, and η) with different coding regions. A high resolution gel electrophoresis system was developed to separate minor 5 S RNA's from the major 5 S RNA (α). A study of several
E U, Selker +2 more
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Plant Small RNAs Responsive to Fungal Pathogen Infection
2018Accumulating evidence indicates that small noncoding RNAs (sRNAs) can be transferred across species for interkingdom communication. In addition to the artificial transgene-derived small interfering RNAs (siRNAs), endogenous microRNAs (miRNAs) can also influence interacting organisms to execute a regulatory function. For instance, we have recently found
Yun, Jin, Hui-Shan, Guo
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A method for the extraction of high quality fungal RNA suitable for RNA-seq
Journal of Microbiological Methods, 2020Transcriptomic analysis is an OMICs technology that is becoming indispensable to understand and get a complete picture of cell functioning and adaptation to the environmental cues the cell is continuously receiving. Among the techniques available to perform transcriptomics, RNA-seq is becoming the method of choice.
Leyda, Cortés-Maldonado +4 more
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2014
This book presents an overview of the RNA networks controlling gene expression in fungi highlighting the remaining questions and future challenges in this area. It covers several aspects of the RNA-mediated mechanisms that regulate gene expression in model yeasts and filamentous fungi, organisms of great importance for industry, medicine and ...
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This book presents an overview of the RNA networks controlling gene expression in fungi highlighting the remaining questions and future challenges in this area. It covers several aspects of the RNA-mediated mechanisms that regulate gene expression in model yeasts and filamentous fungi, organisms of great importance for industry, medicine and ...
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A Plasmodium falciparum protein related to fungal RNA 5′-triphosphatases
Molecular and Biochemical Parasitology, 2001Messenger RNA of eukaryotic cells and most of their viruses carry a ‘cap’ structure at their 5 end. RNA 5 -triphosphatase (RTPase) activity is required for the first step of cap biosynthesis. This activity removes the -phosphate from the 5 end of a nascent mRNA to leave a diphosphate end. mRNA guanylyltransferase (GTase) subsequently adds GMP in a 5 –5
T, Takagi, S, Buratowski
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Fingerprinting Double‐Stranded Non‐Radioactive RNA from a Fungal Virus
European Journal of Biochemistry, 1973The fingerprinting technique has been applied to double‐stranded viral RNA. Under appropriate conditions pancreatic and T1 ribonuclease digests were obtained which upon two‐dimensional paper electrophoresis yielded specific patterns, similar to those obtained from single‐stranded RNAs.
T, Loviny, M, Székely
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