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Evidence that immune RNA is messenger RNA
Cellular Immunology, 1976Abstract Exposure of rabbit spleen cell cultures to i-RNA isolated from T2 phage-exposed rabbit peritoneal exudate cells induces the synthesis of antigen and allotype specific 19S proteins even in the presence of actinomycin D. The same i-RNA directs the synthesis of proteins with comparable properties in cell-free extracts prepared from mouse L ...
Patricia Bilello +3 more
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EcoSal Plus, 2007
This chapter discusses several topics relating to the mechanisms of mRNA decay. These topics include the following: important physical properties of mRNA molecules that can alter their stability; methods for determining mRNA half-lives; the genetics and biochemistry of proteins and enzymes involved in mRNA decay; posttranscriptional ...
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This chapter discusses several topics relating to the mechanisms of mRNA decay. These topics include the following: important physical properties of mRNA molecules that can alter their stability; methods for determining mRNA half-lives; the genetics and biochemistry of proteins and enzymes involved in mRNA decay; posttranscriptional ...
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Immunoglobulin messenger RNAS of T lymphocytes
Molecular Immunology, 1980The potential of T cells to synthesize immunoglobulins was investigated in thymus cells obtained from young mice. Such thymocytes contained very low levels of B cell contamination. It was found by hybridization of thymus RNA with complementary DNAs (cDNAs) synthesized from myeloma κ and H chain mRNAs that these T cells contain relatively large amounts ...
Storb, U, Near, R, Putnam, D, Clagett, J
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Effects of Chemically Modified Messenger RNA on Protein Expression.
Bioconjugate chemistry, 2016Chemically modified nucleotides play significant roles in the effectiveness of mRNA translation. Here, we describe the synthesis of two sets of chemically modified mRNAs [encoding firefly Luciferase (FLuc) and enhanced green fluorescent protein (eGFP ...
Bin Li, Xiao Luo, Yizhou Dong
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Trends in Biochemical Sciences, 1976
Abstract Eukaryotic messenger RNA molecules contain methylated nucleotides, both at their 5′-terminal end in a unique ‘cap' structure, and internally as 6-methyladenylic acid. Similar methylation patterns are.found in heterogeneous nuclear RNA molecules.
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Abstract Eukaryotic messenger RNA molecules contain methylated nucleotides, both at their 5′-terminal end in a unique ‘cap' structure, and internally as 6-methyladenylic acid. Similar methylation patterns are.found in heterogeneous nuclear RNA molecules.
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Vaccination With Messenger RNA
2006As an alternative to DNA-based vaccines, messenger RNA (mRNA)-based vaccines present additional safety features: no persistence, no integration in the genome, no induction of autoantibodies. Moreover, mRNA which are generated by in vitro transcription, are easy to produce in large amounts and very high purity.
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Chapter 25 RNA Decay by Messenger RNA Interferases
2008Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and ...
Christensen-Dalsgaard M +3 more
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Intranuclear Trafficking of Messenger RNA
Critical Reviews in Eukaryotic Gene Expression, 1999Within the nucleus, protein-encoding genes are transcribed into messenger RNA by RNA polymerase II. Messenger RNAs migrate to the cytoplasm, but before reaching their final destination the primary transcripts must undergo a series of modifications that include 5'-capping, splicing, and 3'-cleavage/polyadenylation.
Noélia Custódio +2 more
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A CRISPR cut for messenger RNAs
Lab Animal, 2020To achieve knockdown rather than knockout of particular genes, a new paper demonstrates a CRISPR/Cas13 method that can efficiently edit mRNA in zebrafish, medaka, killifish, and mouse embryos.
Rebecca Leech, Karuna Sampath
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The structure of pre-messenger RNA and messenger RNA from erythroid cells
1977Pre-mRNA fractions (greater than 45 S) were characterized by electron microscopy. High salt concentrations (0.2 M ammonium acetate, pH 8) yield linear molecules of different length (0.5--17 micrometer). In 10% of the molecules a compact-nonlinear contour (cn-contour) is detectable at one end.
A M, Ladhoff +3 more
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