Is DNA metabarcoding an option for formaldehyde‐preserved zooplankton time series?
Abstract The recovery of amplifiable DNA from formaldehyde‐fixed (FF) zooplankton samples has long been considered problematic, but advances in degraded‐DNA retrieval have renewed interest in FF samples. To access the information stored in long‐term zooplankton time series, we evaluated methods for extracting amplifiable DNA from community samples ...
Aitor Albaina +4 more
wiley +1 more source
Modular RNA:DNA Nanostructures Enable Nanopore Profiling of rRNA Processing and rRNA Variants. [PDF]
Boskovic F +9 more
europepmc +1 more source
Data‐Independent Acquisition Mass Spectrometry in Tumor Classification and Cancer Biomarker Research
Abstract Cancer treatment is far from optimal also because current classification systems do not reflect the complex molecular status of the tumor and its phenotype in sufficient detail. To construct molecular tumor classifiers, omics tools provide complex molecular data reflecting many aspects from genotype to phenotype.
Jan Simonik +3 more
wiley +1 more source
<i>In silico</i> and experimental assessment of the 16S-ITS-23S operon as a genetic marker for high-resolution bacterial species identification. [PDF]
Yamakawa AC, Davies M, Gweon HS.
europepmc +1 more source
Ion Activation Methods for Top‐Down Proteomics
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley +1 more source
Multilocus DNA Barcoding Resolves Area-Based Genetic Variation in Freshwater Shrimps (<i>Macrobrachium</i> sp. and <i>Caridina</i> sp.) from Saline and Non-Saline Habitats in Northeastern Thailand. [PDF]
Saengprajak J, Pakdeenarong N.
europepmc +1 more source
Evidence for a Constrained Mutational Pathway to High-Level Spectinomycin Resistance in <i>Neisseria</i>: RpsE Loop 2 Mutations and Associated Growth Costs. [PDF]
Kravtsov DV +3 more
europepmc +1 more source
Ribotyping for Accurate Identification of Infectious Bacteria in Animal-Derived Foods and Laboratory Samples: Implications for Human Health. [PDF]
Seyedi ZP +4 more
europepmc +1 more source

