Results 241 to 250 of about 86,320 (269)

Alternative conformers of 5S ribosomal RNA and their biological relevance

Biochemistry, 1985
Different conformational states of Escherichia coli 5S ribosomal RNA that may participate in protein biosynthesis have been either detected experimentally or predicted on the basis of phylogenetic sequence comparisons. The A conformer exists in a high-salt form (AH) that binds ribosomal proteins and assembles into the 50S subunit and in a low-salt form
Roger A. Garrett, Debra A. Peattie, Merete Mathiesen, Anni Christensen   +3 more
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[49] Primer-directed deletions in 5S ribosomal RNA

1988
Publisher Summary This chapter presents detailed procedure for generating and characterizing deletion mutants in Escherichia coli 5S RNA. There follows a more detailed appraisal of the 5S RNA expression system in plasmids, deletion repair mechanisms in the cell, the possible occurrence of DNA rearrangements, and methods for determining structural ...
Roger A. Garrett, Birte Vester, Jan Christiansen, Jan Egebjerg   +3 more
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The release of 5S RNA from reticulocyte ribosomes

Biochemical and Biophysical Research Communications, 1970
Abstract Reticulocyte ribosomes were dissociated under various conditions and the release of 5S RNA was assayed. The 5S RNA was released from ribosomes suspended in Tris buffer, pH 9.5, containing EDTA or in Tris buffer, pH 9.5, after dialysis against the same buffer.
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Location of the genes for 5S ribosomal RNA in Xenopus laevis

Chromosoma, 1973
In situ hybridization of 5S RNA and cRNA transcribed in vitro from Xenopus laevis 5S DNA shows that 5S DNA is localized at or near the telomere region of the long arm of many, if not all, of the X. laevis chromosomes. No 5S DNA is detected near the nucleolus organizer in the normal X. laevis chromosome complement, but in a X.
Donald D. Brown, Donald D. Brown, Max L. Birnstiel, Max L. Birnstiel, Max L. Birnstiel, Mary Lou Pardue, Mary Lou Pardue   +6 more
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Accessibility of 5S RNA to ribonucleases in Escherichia coli ribosomes

Biochimie, 1971
Summary The accessibility of 5S RNA within 70S ribosomes and 50S subunits to T 1 and pancreatic RNase is studied. It is shown that the ribosomal structure affords a very strong protection to 5S RNA against degradation by ribonucleases. Trypsin digestion can unmask one or several sequences in the 5S RNA which become accessible to pancreatic RNase but
Jean Feunteun, R. Monier
openaire   +3 more sources

The nucleotide sequence of ribosomal 5S RNA from sorrel

Biochimie, 1996
The nucleotide sequence of ribosomal 5S RNA from sorrel (Rumex acetosa) was determined. This sequence shows the highest homology to Chenopodiaceae and Cruciferae ribosomal 5S RNA when compared to other dicotyledones' 5S rRNA.
T. Opiola, K. Szkaradkiewicz, Miroslawa Z. Barciszewska   +2 more
openaire   +3 more sources

Analysis of Transfer RNA and 5S Ribosomal RNA

1997
Analysis of RNA molecules is an area of great interest in the biological and medical sciences. RNA molecules are extremely versatile being able to act as templates for protein biosynthesis, catalyse enzyme-like reactions, and direct their own self-replication [1].
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"Portraying" of plant genomes using polymerase chain reaction amplification of ribosomal 5S genes.

Genome, 1991
Nontranscribed spacers of plant genes coding for ribosomal 5S RNA were amplified using the polymerase chain reaction. Primers were synthesized that were complementary to 3' (direct) and 5' (reverse) ends of the coding region and that are universal for ...
A. Kolchinsky, M. Kolesnikova, E. Ananiev   +2 more
semanticscholar   +1 more source

The location of 5S (ribosomal) RNA genes in Drosophila hydei

Chromosoma, 1975
The location of the 5S ribosomal RNA cistrons in band 2-23B1,2 of the polytene (salivary gland) chromosomes of Drosophila hydei was indicated by in situ hybridization of tritiated low molecular weight RNA fractionated from total in vivo synthesized larval RNA or from in vitro synthesized salivary gland RNA and competition of the hybridization of this ...
H. D. Berendes, C. Alonso
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The conformation of loop E of eukaryotic 5S ribosomal RNA

Biochemistry, 1993
The solution structure of a 27-nucleotide duplex, including the internal loop E from Xenopus laevis 5S ribosomal RNA, has been studied by two-dimensional NMR spectroscopy, followed by restrained molecular dynamics. The highly conserved internal loop closes to form a G.A base pair and a reverse-Hoogsteen A.U base pair.
Brian Wimberly, Ignacio Tinoco, Gabriele Varani   +2 more
openaire   +3 more sources