Results 101 to 110 of about 402,636 (258)

Detecting cells with low RNA content colonizing artworks non-invasively: RNA-FISH [PDF]

, 2017
Various non-invasive RNA-FISH methodologies were tested in this work. They seem to be good alternatives for analyzing the potential biodeteriogenic microorganisms thriving in CH objects.This work was co- financed by FCT Fundação para a Ciência e a ...
Caldeira, Ana T., Candeias, António, González-Pérez, Marina, Pazian, Marlon Félix, Pereira, António, Vieira, Ricardo   +5 more
core  

Cold Induction of EARLI1, a Putative Arabidopsis Lipid Transfer Protein, Is Light and Calcium Dependent [PDF]

, 2004
As sessile organisms, plants must adapt to their environment. One approach toward understanding this adaptation is to investigate environmental regulation of gene expression.
Comai L., Gleave A.P., Henriksson K.N., Lee I., Murashige T., Sambrook J., Sukumaran N.P., Wang H., Wirtz K.W.   +8 more
core   +2 more sources

Targeting the MDM2‐MDM4 interaction interface reveals an otherwise therapeutically active wild‐type p53 in colorectal cancer

Molecular Oncology, EarlyView.
This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini, Giada Mele, Marika Attili, Maria Rita Assenza, Fulvio Saccoccia, Francesca Sardina, Cinzia Rinaldo, Roberto Massari, Nicola Tirelli, Alfredo Pontecorvi, Fabiola Moretti   +10 more
wiley   +1 more source

Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles

Molecular Therapy: Methods & Clinical Development, 2015
RNA delivery is an attractive strategy to achieve transient gene expression in research projects and in cell- or gene-based therapies. Despite significant efforts investigating vector-directed RNA transfer, there is still a requirement for better ...
Anne Prel, Vincent Caval, Régis Gayon, Philippe Ravassard, Christine Duthoit, Emmanuel Payen, Leila Maouche-Chretien, Alison Creneguy, Tuan Huy Nguyen, Nicolas Martin, Eric Piver, Raphaël Sevrain, Lucille Lamouroux, Philippe Leboulch, Frédéric Deschaseaux, Pascale Bouillé, Luc Sensébé, Jean-Christophe Pagès   +17 more
doaj  

Introduction and Expression of a Rabbit β-globin Gene in Mouse Fibroblasts [PDF]

, 1979
The cloned chromosomal rabbit ß-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20
Axel, R., Lacy, E., Maniatis, T., Silverstein, S., Wigler, M., Wold, B.   +5 more
core  

Thermal proteome profiling and proteome analysis using high‐definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next‐generation galeterone analog VNPP433‐3β in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Elevated level of cholesterol is positively correlated to prostate cancer development and disease severity. Cholesterol‐lowering drugs, such as statins, are demonstrated to inhibit prostate cancer. VNPP433‐3β interrupts multiple signaling and metabolic pathways, including cholesterol biosynthesis, AR‐mediated transcription of several oncogenes, mRNA 5′
Retheesh S. Thankan, Elizabeth Thomas, Mehari M. Weldemariam, Puranik Purushottamachar, Weiliang Huang, Maureen A. Kane, Yuji Zhang, Nicholas Ambulos, Bi‐Dar Wang, David Weber, Vincent C. O. Njar   +10 more
wiley   +1 more source

Extensive horizontal gene transfer in cheese-associated bacteria. [PDF]

, 2017
Acquisition of genes through horizontal gene transfer (HGT) allows microbes to rapidly gain new capabilities and adapt to new or changing environments.
Bonham, Kevin S, Dutton, Rachel J, Wolfe, Benjamin E   +2 more
core   +3 more sources

Elucidating prognostic significance of purine metabolism in colorectal cancer through integrating data from transcriptomic, immunohistochemical, and single‐cell RNA sequencing analysis

Molecular Oncology, EarlyView.
Low expression of five purine metabolism‐related genes (ADSL, APRT, ADCY3, NME3, NME6) was correlated with poor survival in colorectal cancer. Immunohistochemistry analysis showed that low NME3 (early stage) and low ADSL/NME6 (late stage) levels were associated with high risk.
Sungyeon Kim, Myunghee Kang, Soyeon Jeong, Jisup Kim, Kyoung Oh Kim, Won‐Suk Lee, Jeong‐Heum Baek, Jung Ho Kim, Seungyoon Nam   +8 more
wiley   +1 more source

‌RNA secondary structure and qRT-PCR analyses pertained to expressed anti-CD25 CAR in NK-92 cell line

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2020
Background and Objectives: Tumor-infiltrating regulatory T (TI-Treg) cells perform the significant function in cancer immune escape. In this study, the third generation CAR construct was designed against human CD25 antigen, the significant cell surface ...
Moein Dehbashi, Zohreh Hojati, Majid Motovali-bashi, Mazdak Ganjalikhani-Hakemi   +3 more
doaj  

Time-resolved NMR monitoring of tRNA maturation

Nature Communications, 2019
Transfer RNA (tRNA) is regulated by RNA modifications. Here the authors employ time-resolved NMR to monitor modifications of yeast tRNAPhe in cellular extracts, revealing a sequential order and cross-talk between modifications.
Pierre Barraud, Alexandre Gato, Matthias Heiss, Marjorie Catala, Stefanie Kellner, Carine Tisné   +5 more
doaj   +1 more source