Results 321 to 330 of about 375,655 (343)

Early DNA-RNA complex from the endogenous RNA-directed DNA polymerase activity of uninfected chicken embryos. [PDF]

Nature New Biology, 1973
WHEN endogenous DNA polymerase activity was isolated from normal uninfected chicken embryos1, we suggested that it was RNA-directed for several reasons. The activity was sensitive to RNAase, but was completely resistant to DNAase and partially resistant to actinomycin D; and the DNA product of the chicken endogenous DNA polymerase activity hybridized ...
C. Kang, H. Temin
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Mechanism of action of Moloney murine leukemia virus RNA-directed DNA polymerase associated RNase H (RNase H I).

Biochemistry, 1981
The mechanism of action of the ribonuclease H (RNase H) activity associated with Moloney murine leukemia virus RNA-directed DNA polymerase (RNase H I) and the two-subunit (alpha beta) form of avian myeloblastosis virus DNA polymerase were compared by ...
G. Gerard
semanticscholar   +1 more source

Innate Reverse Transcriptase Activity of DNA Polymerase for Isothermal RNA Direct Detection

Journal of the American Chemical Society, 2015
RNA detection has become one of the most robust parts in molecular biology, medical diagnostics and drug discovery. Conventional RNA detection methods involve an extra reverse transcription step, which limits their further application for RNA rapid detection.
Cuiping Ma, Xiaotong Shen, Shuyan Niu, Chao Shi   +3 more
openaire   +2 more sources

Characterization of virus-like particles from a psoriatic patient with respect to the possible presence of particle-associated RNA and RNA-directed DNA polymerase.

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology, 2009
Subcellular particles resembling retroviruses with respect to their morphology, density, and protein composition, and which are present in the urine of a psoriatic patient have been analysed for the presence of high-molecular-weight polyadenylated RNA ...
O. Iversen, J. Nissen-Meyer, A. Dalen
semanticscholar   +1 more source

An improved method for the analysis of the RNA-directed DNA polymerase reaction by polyacrylamide gels

Analytical Biochemistry, 1973
Abstract Analysis of the products of the RNA-directed DNA polymerase reaction using glycerol-substituted polyacrylamide gels has proven superior to the glycerol gradient method for quantitative assay of a series of nucleic acid samples. To overcome the phenomenon of a smearing of background radioactive counts in the gels caused by unincorporated 3H ...
J. Justin McCormick, Lenora Calhoun
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Synthesis of Sindbis virus complementary DNA by avian myeloblastosis virus RNA-directed DNA polymerase

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
Sindbis virus 42 S RNA was efficiently transcribed into complementary DNA (CDNA) by avian myeloblastosis virus alphabeta DNA polymerase using oligo- (dT) or single-stranded calf thymus DNA as primers. Both of the Sindbis virus cDNA products were able to protect 60% of 125I-labeled Sindbis virus RNA, at near equal weight ratios, from RNAase A and T1 ...
Vadim Yuferov, Charles H. Riggin, Duane P. Grandgenett, Michael Tigges, Maurice Bondurant   +4 more
openaire   +3 more sources

Molecular mechanisms of the RNA polymerases in plant RNA-directed DNA methylation

Trends in Biochemical Sciences
In plants, two atypical DNA-dependent RNA polymerases, RNA polymerase IV (Pol IV) and Pol V, and an RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) together produce noncoding RNAs (ncRNAs) to guide the plant-specific RNA-directed DNA methylation (RdDM). Although both Pol IV and Pol V have evolved from the canonical Pol II, they have adapted to different roles in
Guohui Xie, Xuan Du, Hongmiao Hu, Jiamu Du   +3 more
openaire   +2 more sources

Total synthesis of (−)-mniopetal F, a novel inhibitor of RNA-directed DNA-polymerases

Tetrahedron, 2001
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Yoshikazu Suzuki, Aiko Ohara, Kenji Sugaya, Kin-ichi Tadano, Ken Ichi Takao   +4 more
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Quantitative structure-activity correlations of rifamycins as inhibitors of viral RNA-directed DNA polymerase and mammalian .alpha. and .beta. DNA polymerases

Journal of Medicinal Chemistry, 1980
Twenty-two 3-substituted rifamycins were tested for inhibition of mammalian alpha and beta DNA polymerase and viral RNA-dependent DNA polymerase ("reverse transcriptase"). Quantitative structure--activity relationships (QSAR) were formulated for the three systems.
R. S. Wu, F. R. Quinn, M. K. Wolpert‐Defilippes   +2 more
openaire   +4 more sources

Elimination of residual RNase activity from purified preparations of RNA-directed DNA polymerase

Biochemical and Biophysical Research Communications, 1975
Abstract Preparations of RNA-directed DNA polymerase purified from RNA tumor viruses by standard methods generally contain trace amounts of single-stranded RNA endonucleolytic activity detectable only by relatively sensitive methods. This contaminating RNase activity has been found to be completely inhibited when RNA-directed DNA polymerase reactions
Marc S. Collett, Anthony J. Faras
openaire   +3 more sources