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RNA-Seq data analysis stands as a vital part of genomics research, turning vast and complex datasets into meaningful biological insights. It is a field marked by rapid evolution and ongoing innovation, necessitating a thorough understanding for anyone seeking to unlock the potential of RNA-Seq data.
James, Li +2 more
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James, Li +2 more
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RNA sequencing (RNA-Seq) has emerged as a powerful and versatile tool for the comprehensive analysis of transcriptomes and has been widely used to investigate gene expression, copy number variation, alternative splicing, and novel transcript discovery. This chapter outlines the methodology for conducting short-read RNA-Seq, starting from RNA enrichment
Rong, Hu +3 more
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Rong, Hu +3 more
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The devil in the details of RNA-seq
Nature Biotechnology, 2014Piero Carninci, Anton Kratz
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A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors
Nature Medicine, 2020Michal Slyper +2 more
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Cross-species RNA-seq for deciphering host–microbe interactions
Nature Reviews Genetics, 2021Alexander J Westermann, Jörg Vogel
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Dual RNA-seq of pathogen and host
Nature Reviews Microbiology, 2012Alexander J Westermann, Jörg Vogel
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Full-length RNA-seq from single cells using Smart-seq2
Nature Protocols, 2014Simone Picelli +2 more
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