Results 1 to 10 of about 1,056,288 (288)

RNA extraction from self-assembling peptide hydrogels to allow qPCR analysis of encapsulated cells. [PDF]

PLoS ONE, 2018
Self-assembling peptide hydrogels offer a novel 3-dimensional platform for many applications in cell culture and tissue engineering but are not compatible with current methods of RNA isolation; owing to interactions between RNA and the biomaterial.
Kyle A Burgess, Victoria L Workman, Mohamed A Elsawy, Aline F Miller, Delvac Oceandy, Alberto Saiani   +5 more
doaj   +4 more sources

Simple Method for Efficient RNA Extraction From Arabidopsis Embryos [PDF]

Bio-Protocol
Plant embryos are contained within seeds. Isolating them is crucial when endosperm and seed coat tissues interfere with the study of mutant genetic functions due to differing genotypes between maternal and embryonic tissues.
Fernanda Marchetti, Gabriela Pagnussat, Eduardo Zabaleta   +2 more
doaj   +2 more sources

Comparing RNA extraction protocols from formalin-fixed paraffin-embedded microcore samples. [PDF]

PLoS One
Golias M, Krupova Z, Defrenaix P, Heymann MF, Heymann D.   +4 more
europepmc   +2 more sources

Optimized Protocol for High-Quality RNA Extraction from Grape Berry Skins Using Sorbitol Pre-Wash. [PDF]

Plants (Basel)
Prencipe A, Salerno A, D'Amico M, Marsico AD, Ventura M, Velasco R, Cardone MF, Bergamini C, Maggiolini FAM.   +8 more
europepmc   +3 more sources

Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method

Plant Varieties Studying and Protection, 2021
Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the ...
М. Ізейрай
doaj   +1 more source

1 to 1, 11,111 Covid sample testing; Decoding the testing matrix!

Asian Journal of Medical Sciences, 2021
Almost 100 yrs. after the 1918 Spanish Flu Pandemic, world is witnessing “Covid-19”, another Pandemic of similar scale. Interesting, parallels are being drawn between these two pandemics –occurring a century apart; on their scale of spread, potential ...
Chaitali Nikam , Caesar Sengupta , Kallathikumar K , Krishnakumar S , PraveenKumar Ganesan   +4 more
doaj   +1 more source

High RNA quality extracted from the tolerant crop Cyamopsis tetragonoloba (L.) despite possession of low RNA integrity number

Biotechnology & Biotechnological Equipment, 2021
Salinity is one of the most limiting constraints of crop production. Nevertheless, highly-tolerant crop plants (e.g. ‘Guar’ Cyamopsis tetragonoloba) can thrive well under salinity conditions.
Abdel-Rhman Zakaria Gaafar, Fahad Al-Qurainy, Aref Alshameri, Salim Khan, Mohammad Nadeem, Mohamed Tarroum, Saleh Alansi, Hassan O. Shaikhaldein, Abdalrhaman M. Salih, Norah Arrak Alenezi   +9 more
doaj   +1 more source

Clinical Evaluation of Locally Made Flocked Swabs in Response to the COVID-19 Pandemic in a Developing Country

International Journal of Technology, 2020
The coronavirus disease 2019 (COVID-19) pandemic has caused an international shortage of nasopharyngeal flocked swabs, which are one of the main supplies for diagnostic testing.
Narottama Tunjung, Prasetyanugraheni Kreshanti, Yulia Rosa Saharman, Yudan Whulanza, Sugeng Supriadi, Mochammad Chalid, Margareth Ingrid Anggraeni, Agus Rizal A. H. Hamid, Chaula Luthfia Sukasah   +8 more
doaj   +1 more source

Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans

Plant Production Science, 2021
Isolation of an adequate quantity of high-quality RNA is a crucial step in gene expression analysis. Difficulties in sampling and RNA extraction of field-grown crops, especially from roots, are substantial obstacles to understanding actual gene ...
Shin Okamura, Nabila Mumtahina, Hiroyuki Shimono, Maya Matsunami   +3 more
doaj   +1 more source

Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument

Indonesian Journal of Medical Laboratory Science and Technology, 2021
RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires ...
Muhammad Taufiq Hidayat, Endry Nugroho Prasetyo   +1 more
doaj   +1 more source