Results 1 to 10 of about 356,994 (139)

RNA extraction from self-assembling peptide hydrogels to allow qPCR analysis of encapsulated cells. [PDF]

open access: yesPLoS ONE, 2018
Self-assembling peptide hydrogels offer a novel 3-dimensional platform for many applications in cell culture and tissue engineering but are not compatible with current methods of RNA isolation; owing to interactions between RNA and the biomaterial.
Kyle A Burgess   +5 more
doaj   +4 more sources

Optimization of phenol-chloroform RNA extraction

open access: yesMethodsX, 2018
Accurate and reliable analysis of gene expression depends on the extraction of pure and high-quality RNA. However, while the conventional phenol-chloroform RNA extraction is preferable over silica-based columns, particularly when cost is a concern or ...
Lee S. Toni   +6 more
doaj   +3 more sources

Sampling for vegetative propagation: A phytosanitary status survey of grapevines collection by One Step RT-PCR method

open access: yesPlant Varieties Studying and Protection, 2021
Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the ...
М. Ізейрай
doaj   +1 more source

High RNA quality extracted from the tolerant crop Cyamopsis tetragonoloba (L.) despite possession of low RNA integrity number

open access: yesBiotechnology & Biotechnological Equipment, 2021
Salinity is one of the most limiting constraints of crop production. Nevertheless, highly-tolerant crop plants (e.g. ‘Guar’ Cyamopsis tetragonoloba) can thrive well under salinity conditions.
Abdel-Rhman Zakaria Gaafar   +9 more
doaj   +1 more source

1 to 1, 11,111 Covid sample testing; Decoding the testing matrix!

open access: yesAsian Journal of Medical Sciences, 2021
Almost 100 yrs. after the 1918 Spanish Flu Pandemic, world is witnessing “Covid-19”, another Pandemic of similar scale. Interesting, parallels are being drawn between these two pandemics –occurring a century apart; on their scale of spread, potential ...
Chaitali Nikam   +4 more
doaj   +1 more source

Clinical Evaluation of Locally Made Flocked Swabs in Response to the COVID-19 Pandemic in a Developing Country

open access: yesInternational Journal of Technology, 2020
The coronavirus disease 2019 (COVID-19) pandemic has caused an international shortage of nasopharyngeal flocked swabs, which are one of the main supplies for diagnostic testing.
Narottama Tunjung   +8 more
doaj   +1 more source

Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans

open access: yesPlant Production Science, 2021
Isolation of an adequate quantity of high-quality RNA is a crucial step in gene expression analysis. Difficulties in sampling and RNA extraction of field-grown crops, especially from roots, are substantial obstacles to understanding actual gene ...
Shin Okamura   +3 more
doaj   +1 more source

Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument

open access: yesIndonesian Journal of Medical Laboratory Science and Technology, 2021
RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires ...
Muhammad Taufiq Hidayat   +1 more
doaj   +1 more source

Optimal RNA Extraction Methods and Development of Synthetic Clones for Seven Strawberry Viruses

open access: yesResearch in Plant Disease, 2020
Most strawberry viruses exist relatively low titers in tissues, and strawberry tissues include high levels of contamination by polysaccharides and phenolic compounds. These traits make the efficiency of strawberry diagnosis difficult.
Sun-Jung Kwon   +3 more
doaj   +1 more source

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