Results 1 to 10 of about 1,042,593 (334)
RNA extraction from self-assembling peptide hydrogels to allow qPCR analysis of encapsulated cells. [PDF]
PLoS ONE, 2018 Self-assembling peptide hydrogels offer a novel 3-dimensional platform for many applications in cell culture and tissue engineering but are not compatible with current methods of RNA isolation; owing to interactions between RNA and the biomaterial.
Kyle A Burgess +5 more
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Simple Method for Efficient RNA Extraction From Arabidopsis Embryos [PDF]
Bio-ProtocolPlant embryos are contained within seeds. Isolating them is crucial when endosperm and seed coat tissues interfere with the study of mutant genetic functions due to differing genotypes between maternal and embryonic tissues.
Fernanda Marchetti +2 more
doaj +2 more sources
Viral RNA extraction for in-the-field analysis [PDF]
Journal of Virological Methods, 2007 Retroviruses encode their genetic information with RNA molecules, and have a high genomic recombination rate which allows them to mutate more rapidly, thereby posting a higher risk to humans. One important way to help combat a pandemic of viral infectious diseases is early detection before large-scale outbreaks occur. The polymerase chain reaction (PCR)
Jiang F. Zhong +3 more
openaire +3 more sources
A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenografts. [PDF]
, 2016 Next-generation sequencing (NGS) can identify and validate new biomarkers of cancer onset, progression and therapy resistance. Substantial archives of formalin-fixed, paraffin-embedded (FFPE) cancer samples from patients represent a rich resource for ...
Adamian, Yvess +12 more
core +4 more sources
Optimization of phenol-chloroform RNA extraction
MethodsX, 2018 Accurate and reliable analysis of gene expression depends on the extraction of pure and high-quality RNA. However, while the conventional phenol-chloroform RNA extraction is preferable over silica-based columns, particularly when cost is a concern or ...
Lee S. Toni +6 more
doaj +3 more sources
1 to 1, 11,111 Covid sample testing; Decoding the testing matrix!
Asian Journal of Medical Sciences, 2021 Almost 100 yrs. after the 1918 Spanish Flu Pandemic, world is witnessing “Covid-19”, another Pandemic of similar scale. Interesting, parallels are being drawn between these two pandemics –occurring a century apart; on their scale of spread, potential ...
Chaitali Nikam +4 more
doaj +1 more source
Biotechnology & Biotechnological Equipment, 2021 Salinity is one of the most limiting constraints of crop production. Nevertheless, highly-tolerant crop plants (e.g. ‘Guar’ Cyamopsis tetragonoloba) can thrive well under salinity conditions.
Abdel-Rhman Zakaria Gaafar +9 more
doaj +1 more source
Plant Varieties Studying and Protection, 2021 Purpose. Grapevines (Vitis spp.) are affected by many viral diseases which cause serious pathological problems. GLRaV-3 is among the most widespread leafroll viruses, while Grapevine Fanleaf Virus (GFLV) is a destructive pathogen which reduces the ...
М. Ізейрай
doaj +1 more source
Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans
Plant Production Science, 2021 Isolation of an adequate quantity of high-quality RNA is a crucial step in gene expression analysis. Difficulties in sampling and RNA extraction of field-grown crops, especially from roots, are substantial obstacles to understanding actual gene ...
Shin Okamura +3 more
doaj +1 more source