Results 91 to 100 of about 356,994 (139)
Evaluation of three RNA extraction methods from three cultivars of Malaysian upland rice [PDF]
Rice (Oryza sativa L.) can be divided into two major categories, which is upland rice and lowland rice. Apart from being the staple food for more than half of the world population, it is also known as model plant for functional genomics study.
Arif, S. M.+3 more
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Evaluation and Optimization of Various Procedures of RNA Extraction from Human Frozen Tissues
• Background: Utilizing an intact RNA is essential while performing molecular genetics methods like microarray and quantitative Real-time polymerase chain reaction (RT-PCR).
Faezeh Mohammad Hashem+2 more
doaj
Role of the Mitochondrial Genome During Early Development in Mice [PDF]
The role of the mitochondrial genome in early development and differentiation was studied in mouse embryos cultured in vitro from the two to four cell stage to the blastocyst (about 100 cells).
Chase, David G., Pikó, Lajos
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RNA extraction with PGTX v2 [PDF]
This protocol is used for RNA extraction in cyanobacteria after Pinto et al. 2009. Pinto, Fernando Lopes; Thapper, Anders; Sontheim, Wolfgang; Lindblad, Peter (2009): Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria. In: BMC molecular biology 10, S. 79. DOI: 10.1186/1471-2199-10-79 .
openaire +2 more sources
Optimization of RNA Extraction from Rat Pancreatic Tissue
Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any ...
Sanaz Dastgheib+4 more
doaj
RNA extraction with PGTX v1 [PDF]
This protocol is used for RNA extraction in cyanobacteria after Pinto et al. 2009. Pinto, Fernando Lopes; Thapper, Anders; Sontheim, Wolfgang; Lindblad, Peter (2009): Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria. In: BMC molecular biology 10, S. 79. DOI: 10.1186/1471-2199-10-79 .
openaire +1 more source
Creating E.coli Library of Diagnostic Gene for Influenza B Virus Using Molecular Cloning Technique [PDF]
Influenza viruses cause seasonal epidemics associated with high morbidity, especially in immunocompromised and extreme age group individuals. Many strategies for combating these viruses are in practice; however, developing and maintaining sensitive ...
Chinbat, Oyunsuvd
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Archival fixed tissues hold key insights into the evolutionary history of RNA viruses and the associated host immune response, yet access to the RNA sequence data is limited by a lack of robust methods for RNA extraction and sequence retrieval from these
Tess Wilson+6 more
doaj +1 more source
This protocol is adapted from the QIAGEN RNeasy Plant mini kit (Jan 2011). Specific modifications have been made to apply this RNA extraction to tobacco pericarp or fruit tissue.
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Optimized protocol for DNA/RNA co-extraction from adults of Dirofilaria immitis
Dirofilaria immitis, the etiologic agent of canine heartworm disease, like several other filarial nematodes, harbors the bacterial endosymbiont Wolbachia. To investigate metabolic and functional pathways of D.
Chiara Lucchetti+5 more
doaj