Results 101 to 110 of about 356,994 (139)
, 2018 This protocol was based on a 2010 paper by Meng and Feldman for extraction of RNA from arabidopsis siliques. While the protocol remains unchanged, I have made some slight edits to streamline it in the lab and also added some notes regarding my results applying this protocol to tobacoo fruit tissue.
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Detection and quantitation of PPR RNA using realtime RT-PCR after maual and automated sample processing [PDF]
, 2008 Albina, Emmanuel +4 more
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Exploiting grammatical relations for protein relation extraction and role labeling [PDF]
, 2008 Cornelis, Chris +3 more
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, 2007 This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.
Helen E. Conlon, Michael G. Salter
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2006
RNA extraction is a routine technique in a molecular biology lab. High quality of RNA extracted from plants is a prerequisite to succeeding in subsequent experiments such as reverse transcription-polymerase chain reaction, Northern hybridization, cDNA library construction, and microarray analysis.
Huazhong, Shi, Ray, Bressan
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RNA extraction is a routine technique in a molecular biology lab. High quality of RNA extracted from plants is a prerequisite to succeeding in subsequent experiments such as reverse transcription-polymerase chain reaction, Northern hybridization, cDNA library construction, and microarray analysis.
Huazhong, Shi, Ray, Bressan
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2006
DNA microarrays enable insights into global gene expression by capturing a snapshot of cellular expression levels at the time of sample collection. Careful RNA handling and extraction are required to preserve this information properly, ensure sample-to-sample reproducibility, and limit unwanted technical variation in experimental data.
Bernard F. Andruss +2 more
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DNA microarrays enable insights into global gene expression by capturing a snapshot of cellular expression levels at the time of sample collection. Careful RNA handling and extraction are required to preserve this information properly, ensure sample-to-sample reproducibility, and limit unwanted technical variation in experimental data.
Bernard F. Andruss +2 more
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2004
The direct isolation of RNA from cartilage has often proved difficult owing to a number of factors. Cartilage has a low cell content and contains an extracellular matrix rich in proteoglycans, which copurify with the RNA as they are large and negatively charged macromolecules.
Mallein-Gerin, F., Gouttenoire, J.
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The direct isolation of RNA from cartilage has often proved difficult owing to a number of factors. Cartilage has a low cell content and contains an extracellular matrix rich in proteoglycans, which copurify with the RNA as they are large and negatively charged macromolecules.
Mallein-Gerin, F., Gouttenoire, J.
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2003
Current research into the structure and function of plant genes involves the application of many elaborate techniques for gene cloning and analysis. The isolation of pure, intact plant mRNA is required at many stages in this process, e.g., for generation and screening of cDNA clones, for characterization and mapping of cloned genes, and for the study ...
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Current research into the structure and function of plant genes involves the application of many elaborate techniques for gene cloning and analysis. The isolation of pure, intact plant mRNA is required at many stages in this process, e.g., for generation and screening of cDNA clones, for characterization and mapping of cloned genes, and for the study ...
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Avian Influenza Virus RNA Extraction
2014The efficient extraction and purification of viral RNA is critical for downstream molecular applications such as the sensitive and specific detection of virus in clinical samples, virus gene cloning and expression, gene sequencing, or quantification of avian influenza (AI) virus by molecular methods from experimentally infected birds.
Erica Spackman, Scott A. Lee
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