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The Extraction and Fractionation of RNA
1983Biological materials contain two forms of nucleic acid, namely DNA and RNA. It has been known for a number of years that RNA molecules are involved in the expression of the genetic material (DNA) by directing protein synthesis. Knowledge of the metabolism and mode of action of RNA is therefore crucial to our understanding of cell division, growth and ...
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Urea Extraction of Reovirus RNA
Proceedings, annual meeting, Electron Microscopy Society of America, 1967The aim of our study was to determine the total length of reovirus RNA and to better understand the breakage of this double-stranded RNA into smaller pieces. The protein-monolayer technique with urea as a denaturative agent was used in the study of reovirus RNA (type 3). In one series of experiments we incubated the purified virus in 4M urea at 4°C for
A. K. Kleinschmidt, C. Vasquez
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DNA/RNA Extraction and Precipitation
1986This chapter presents a method for DNA/RNA extraction and precipitation. This method involves the removal of protein and contaminants from samples of DNA or RNA prior to use. For this purpose, an SS-phenol/chloroform extraction of protein is followed by ethanol precipitation. The preparation for extraction and precipitation involves an aqueous solution
James F. Battey +2 more
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Hordeivirus Isolation and RNA Extraction [PDF]
, 1998 Diane M. Lawrence, Andrew O. Jackson
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RNA Extraction From Drosophila Heads
2007In Drosophila, input, pacemaker, and output genes are expressed circadianly. mRNA oscillations contribute largely to these rhythms. Determining RNA levels of circadian genes is thus frequently necessary to understand their regulation, or the effect of mutations and genetic manipulations on the function of the circadian pacemaker.
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