Results 161 to 170 of about 1,068,109 (284)

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Monkeypox Viral Nucleic Acids Detected Using Both DNA and RNA Extraction Workflows [PDF]

Sci Total Environ, 2023
Sharkey ME, Babler KM, Shukla BS, Abelson SM, Alsuliman B, Amirali A, Comerford S, Grills GS, Kumar N, Laine J, Lee J, Lamar WE, Mason CE, Penso J, Reding BD, Schürer S, Stevenson M, Vidovic D, Solo-Gabriele H.   +18 more
europepmc   +2 more sources

Impact of RNA extraction on respiratory microbiome analysis using third-generation sequencing. [PDF]

BMC Genomics
Michel A, Leoz M, Nesi N, Petat H, Ar Gouilh M, Charbonnier Le Clezio C, Marguet C, Hassel C, Plantier JC.   +8 more
europepmc   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

Minimally invasive autopsy as a useful tool for RNA extraction in molecular pathology of lung diseases. [PDF]

Braz J Med Biol Res
Batah SS, Fabro AT.
europepmc   +1 more source

Evolutionary dynamics of the chloroplast genome in Daphne (Thymelaeaceae): comparative analysis with related genera and insights into phylogenetics

FEBS Open Bio, EarlyView.
Comparative analysis of chloroplast genomes from 14 genera of Thymelaeaceae revealed variation in gene content, ranging from 128 to 142 genes, primarily influenced by IR expansion/contraction events and pseudogenization of ndhF, ndhI, and ndhG. Two large inversions were detected within the large single‐copy region, including a synapomorphic inversion ...
Abdullah, Hui Li, Rushan Yan, Chengyu Chen, Madiha Islam, Abdul Majid, Ibrar Ahmed, Parviz Heidari, Xiaoxuan Tian   +8 more
wiley   +1 more source

Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing. [PDF]

BMC Res Notes
Sivaprakasam S, Yee Fan T, Kumarasan Y, Hata EM, Vadamalai G, Petersen B, Sicheritz-Pontén T, Parimannan S, Rajandas H.   +8 more
europepmc   +1 more source

RNA Extraction from Cartilage: Issues, Methods, Tips. [PDF]

Int J Mol Sci, 2023
Pagani S, Maglio M, Sicuro L, Fini M, Giavaresi G, Brogini S.   +5 more
europepmc   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Evaluation of RNA extraction and rRNA depletion protocols for RNA-Seq in eleven edible seaweed species from brown, red, and green algae. [PDF]

PLoS One
Dekker RJ, Ensink WA, van Olst MF, van Leeuwen SM, de Leeuw WC, Jonker MJ, Breit TM.   +6 more
europepmc   +1 more source