Results 171 to 180 of about 1,056,288 (288)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

open access: yesFEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Monkeypox Viral Nucleic Acids Detected Using Both DNA and RNA Extraction Workflows [PDF]

open access: yesSci Total Environ, 2023
Sharkey ME   +18 more
europepmc   +2 more sources

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Impact of RNA extraction on respiratory microbiome analysis using third-generation sequencing. [PDF]

open access: yesBMC Genomics
Michel A   +8 more
europepmc   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

open access: yesFEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas   +6 more
wiley   +1 more source

RNA Extraction from Cartilage: Issues, Methods, Tips. [PDF]

open access: yesInt J Mol Sci, 2023
Pagani S   +5 more
europepmc   +1 more source

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