Results 181 to 190 of about 1,056,288 (288)

Optimized cultivation for RNA extraction and cDNA synthesis of Moniliophthora perniciosa (Stahel) Aime e Phillips-Mora for recombinant production of a cellulase

open access: gold, 2021
Edjane Bastos Ferreira   +4 more
openalex   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

open access: yesFEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan   +6 more
wiley   +1 more source

Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing. [PDF]

open access: yesBMC Res Notes
Sivaprakasam S   +8 more
europepmc   +1 more source

Peer Review #2 of "A robust method for RNA extraction and purification from a single adult mouse tendon (v0.2)"

open access: gold, 2018
Mor Grinstein   +7 more
openalex   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

open access: yesFEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp   +13 more
wiley   +1 more source

RT-dPCR in Mosquito Samples for ZIKV Detection: Effects of RNA Extraction and Reverse Transcription in Target Concentration [PDF]

open access: gold, 2020
Paula Rodrigues de Almeida   +3 more
openalex   +1 more source

Evolutionary dynamics of the chloroplast genome in Daphne (Thymelaeaceae): comparative analysis with related genera and insights into phylogenetics

open access: yesFEBS Open Bio, EarlyView.
Comparative analysis of chloroplast genomes from 14 genera of Thymelaeaceae revealed variation in gene content, ranging from 128 to 142 genes, primarily influenced by IR expansion/contraction events and pseudogenization of ndhF, ndhI, and ndhG. Two large inversions were detected within the large single‐copy region, including a synapomorphic inversion ...
Abdullah   +8 more
wiley   +1 more source

Portable, precise, and RNA extraction-free: RT-RAA technology for rapid early RSV identification and prevention. [PDF]

open access: yesFront Cell Infect Microbiol
Wang Z   +8 more
europepmc   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

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