Results 11 to 20 of about 1,042,593 (334)

Clinical Evaluation of Locally Made Flocked Swabs in Response to the COVID-19 Pandemic in a Developing Country

International Journal of Technology, 2020
The coronavirus disease 2019 (COVID-19) pandemic has caused an international shortage of nasopharyngeal flocked swabs, which are one of the main supplies for diagnostic testing.
Narottama Tunjung, Prasetyanugraheni Kreshanti, Yulia Rosa Saharman, Yudan Whulanza, Sugeng Supriadi, Mochammad Chalid, Margareth Ingrid Anggraeni, Agus Rizal A. H. Hamid, Chaula Luthfia Sukasah   +8 more
doaj   +1 more source

Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument

Indonesian Journal of Medical Laboratory Science and Technology, 2021
RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires ...
Muhammad Taufiq Hidayat, Endry Nugroho Prasetyo   +1 more
doaj   +1 more source

RNA extraction and purification

, 2022
Protocol for RNA extraction from cell lines and purification for RNA-Seq.
Klimek, Christoph, Onstein, Svenja, Barbosa, Barbara, Wolf, Gernot   +3 more
openaire   +1 more source

Optimization of a molecular diagnostic strategy to verify SARS-CoV-2 infections by RT-qPCR

Journal of Laboratory Medicine, 2020
Fast and precise detection of SARS-CoV-2 RNA in infected patients is essential for treatment decisions.
Noßmann Marcel
doaj   +1 more source

Protocol Development for Yielding High Quality DNA and RNA from Archived Formalin-Fixed Paraffin Embedded Tissues

Translation, 2023
Introduction: While genetic analysis of archived formalin-fixed paraffin embedded (FFPE) tissue specimens would be a significant research resource, extraction of high-quality DNA and RNA from these specimens is a significant challenge. Storage duration,
Sara Kazmi, Bella Khatib-Shahidi, John Najjar, Anish Sharma, Caitlyn Murphy, Humza Bashir, Benjamin W. French, Apurva Lad, David Kennedy, Steven Haller   +9 more
doaj   +1 more source

Optimal RNA Extraction Methods and Development of Synthetic Clones for Seven Strawberry Viruses

Research in Plant Disease, 2020
Most strawberry viruses exist relatively low titers in tissues, and strawberry tissues include high levels of contamination by polysaccharides and phenolic compounds. These traits make the efficiency of strawberry diagnosis difficult.
Sun-Jung Kwon, Ju-Yeon Yoon, In-Sook Cho, Bong-Nam Chung   +3 more
doaj   +1 more source

Improved CTAB method for RNA extraction of thick waxy leaf tissues from sago palm (Metroxylon sagu Rottb.)

Chemical and Biological Technologies in Agriculture, 2022
Background There is a growing interest in transcriptomics studies parallel to the advancement of transcriptome databases and bioinformatics, which provided the opportunity to study responses to growths, stimuli and stresses.
Wei-Jie Yan, Fifi Hafizzah Pendi, Hasnain Hussain   +2 more
doaj   +1 more source

Comparison of five assays for DNA extraction from bacterial cells in human faecal samples [PDF]

, 2020
Aim To determine the most effective DNA extraction method for bacteria in faecal samples. Materials and Results This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit ...
Glorieux, Griet, Gryp, Tessa, Joossens, M., Vaneechoutte, Mario   +3 more
core   +1 more source

Isolation of high quality RNA from soil-grown Ilex paraguariensis roots suitable for next-generation sequencing and gene expression analyses [PDF]

, 2017
Extraction of high quality RNA is a prerequisite for downstream application in functional genomics analyses. However, the extraction and purification of pure nucleic acids from root tissues is generally difficult due to the high concentration of ...
Acevedo, Raúl Maximiliano, Avico, Edgardo Hernán, Calzadilla, Pablo Ignacio, Ruiz, Oscar Adolfo, Sansberro, Pedro Alfonso   +4 more
core   +1 more source

Comparison of Modified Manual Acid-Phenol Chloroform Method and Commercial RNA Extraction Kits for Resource Limited Laboratories

International Journal of Clinical Practice, 2023
Background and Aim. RNA extraction is a commonly used technique in molecular biology. In recent years, commercially available RNA extraction kits have largely replaced conventional approaches.
Samuel Asamoah Sakyi, Alfred Effah, Emmanuel Naturinda, Ebenezer Senu, Stephen Opoku, Benjamin Amoani, Samuel Kekeli Agordzo, Oscar Simon Olympio Mensah, James Grant, Elizabeth Abban, Tonnies Abeku Buckman, Alexander Kwarteng, Richard K. Dadzie Ephraim, Kwabena Owusu Danquah   +13 more
doaj   +1 more source