Results 271 to 280 of about 1,056,288 (288)

Developing an efficient protocol for RNA extraction from Morelet's crocodile caudal scute biopsies

A.M. Buenfil-Rojas, Maurício González-Jaurégui, Mari Ochiai, Hisato Iwata   +3 more
openalex   +1 more source

Differentiation Treatment Applied to Lung Cancer Model Reduces Pathogenic Traits in Vitro

Advanced Biology, EarlyView.
Non‐small cell lung cancer cells (A549) treated with a differentiation medium showed strong reduction in proliferation, migration, clonogenicity, spheroid formation and stemness markers, with enhanced adhesion properties and alveolar features, all pointing to a notably less aggressive phenotype in support of differentiation therapy as a promising ...
Alice Grossi, Paola Fulghieri, Abdurakhmon Aduvaliev, Karen Soffiantini, Irene Oldrati, Margherita Cavallo, Marco Biggiogera, Giorgia Pellavio, Umberto Laforenza, Monica Savio, Virginie Sottile   +10 more
wiley   +1 more source

Evaluating and optimizing Acid-pH and Direct Lysis RNA extraction for SARS-CoV-2 RNA detection in whole saliva. [PDF]

Sci Rep
LaBute B, Fong J, Ziaee F, Gombar R, Stover M, Beaudin T, Badalova M, Geng Q, Corchis-Scott R, Podadera A, Lago K, Xu Z, Lim F, Chiu F, Fu M, Nie X, Wu Y, Quan C, Hamm C, McKay RM, Ng K, Porter LA, Tong Y.   +22 more
europepmc   +1 more source

3D Bioprinting of Thick Adipose Tissues with Integrated Vascular Hierarchies

Advanced Functional Materials, Volume 35, Issue 12, March 18, 2025.
An advanced 3D bioprinting technique is used here to create thick adipose tissues with a central, vessel and extensive branching. The construct is made using alginate, gelatin and collagen‐based bioinks. Flow through the complex vessel network is demonstrated as well as its successful integration with a femoral artery following implantation in a rat ...
Idit Goldfracht, Majd Machour, Inbal Michael, Maria Bulatova, Janette Zavin, Shulamit Levenberg   +5 more
wiley   +1 more source

Rapid Detection of Severe Fever with Thrombocytopenia Syndrome Virus in the Acute Phase of Infection by Direct Real-Time Reverse Transcription without RNA Extraction. [PDF]

Am J Trop Med Hyg
Hashimoto T, Yahiro T, Ono K, Takenaka R, Demetria C, Khan S, Kimitsuki K, Abe R, Hiramatsu K, Nishizono A.   +9 more
europepmc   +1 more source

A Vascularized Microphysiological System Reproducing Endochondral Ossification in Vitro to Study Ewing Sarcoma Proliferation and Migration

Advanced Functional Materials, EarlyView.
A biofabricated 3D in vitro model recapitulating endochondral ossification (ECO) is described, mimicking the steps from condensation to chondrogenesis and hypertrophy, culminating with vascularization of the hypertrophic construct. As a model proof of concept application, Ewing Sarcoma cells are seeded in the model, showing modifications in their ...
Maria Vittoria Colombo, Chiara Arrigoni, Tobias Faehling, Antonietta Verrillo, Viviana Secci, Thomas G. P. Grünewald, Giuseppe Talò, Alexandra Kummer, Jorge Gonzalez, Vincenzo De Rosa, Christian Candrian, Andrea Barbero, Florencia Cidre‐Aranaz, Matteo Moretti   +13 more
wiley   +1 more source

Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells

Advanced Functional Materials, EarlyView.
CRISPR machinery in diverse molecular formats (DNA, RNA, and ribonucleic protein) is complexed into nanoparticles with the cell‐friendly arginine‐alanine‐leucine‐alanine (RALA) cell‐penetrating peptide. Nanoparticles are delivered to primary mesenchymal stem cells ex vivo or locally in vivo to facilitate multimodal CRISPR gene editing. This RALA‐CRISPR
Joshua P. Graham, Jose Gabriel Castro, Lisette C. Werba, Joseph Amitrano, Luke Fardone, Kevin P. Francis, Anand Ramamurthi, Michael J. Layden, Helen O. McCarthy, Tomas Gonzalez‐Fernandez   +9 more
wiley   +1 more source

A cost-effective RNA extraction and RT-qPCR approach to detect California serogroup viruses from pooled mosquito samples. [PDF]

Sci Rep
Avramov M, Gallo V, Gross A, Lapen DR, Ludwig A, Cullingham CI.   +5 more
europepmc   +1 more source

Detection and quantitation of PPR RNA using realtime RT-PCR after maual and automated sample processing [PDF]

, 2008
Albina, Emmanuel, Keita, Djénéba, Kwiatek, Olivier, Libeau, Geneviève, Servan de Almeida, Renata   +4 more
core  

Plasma‐Treated Hydrogel for Combined RONS and Chemotherapy Delivery: A Proof‐of‐Concept In Ovo

Advanced Functional Materials, EarlyView.
This study explores plasma‐treated hydrogels (PTH) as a new way to deliver both reactive oxygen and nitrogen species and chemotherapy (Doxorubicin) directly to tumors. In ovo tests show effects after a single dose, especially in osteosarcoma tumors. Tumor weight decrease, and a resistance‐related protein is reduced.
Milica Živanić, Albert Espona‐Noguera, Angela Privat Maldonado, Patrik Matušů, Francesco Tampieri, Maria‐Pau Ginebra, Abraham Lin, Evelien Smits, Annemie Bogaerts, Cristina Canal   +9 more
wiley   +1 more source