Biotechnology & Biotechnological Equipment, 2023 High-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS for the extraction of intact RNA from Pseudomonas aeruginosa and compared its performance to established rapid ...
Ivan Stoikov +5 more
doaj +1 more source
Rapid plant DNA and RNA extraction protocol using a bench drill [PDF]
, 2019 Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after.
Amorim, Edson Perito +11 more
core +3 more sources
RNA preservation agents and nucleic acid extraction method bias perceived bacterial community composition. [PDF]
PLoS ONE, 2015 Bias is a pervasive problem when characterizing microbial communities. An important source is the difference in lysis efficiencies of different populations, which vary depending on the extraction protocol used.
Ann McCarthy +3 more
doaj +1 more source
An improved procedure for the isolation of Ribonucleic acid from methicillin-resistant Staphylococcus aureus [PDF]
Novel Research in Microbiology Journal, 2020 Extraction and purification of ribonucleic acid (RNA) from Gram-positive methicillin resistant Staphylococcus aureus (MRSA) is problematic, because the MRSA has a rigid cell wall that contains lipoteichoic acid and peptidoglycan, thus causing ...
Muhanna M. Alshaibani +4 more
doaj +1 more source
Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality? [PDF]
, 2016 Background: Advanced genomic techniques such as Next-Generation-Sequencing (NGS) and gene expression profiling, including NanoString, are vital for the development of personalised medicines, as they enable molecular disease classification.
A Kalmar +31 more
core +1 more source
Extracting Small RNAs from Human Biological Fluids for Subsequent Next-Generation Sequencing
Креативная хирургия и онкология, 2019 A number of questions arise when choosing methods for experiments related to next-generation sequencing. On the one hand, while working with RNA extraction, added reagents and their residues can often inhibit sensitive chemicals with which the sequential
O. A. Beylerli +2 more
doaj +1 more source
Rapid bedside inactivation of Ebola virus for safe nucleic acid tests [PDF]
, 2016 Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport and high-throughput or rapid diagnostic testing during an outbreak.
Bragstad, Karoline +9 more
core +2 more sources
A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses [PDF]
, 2005 http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010&lng=es&nrm=isoPrunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family.
Baeza-Yates, Ricardo +9 more
core +2 more sources
Assessment of RNA extraction protocols from cladocerans.
PLoS ONE, 2022 The usage of cladocerans as non-model organisms in ecotoxicological and risk assessment studies has intensified in recent years due to their ecological importance in aquatic ecosystems.
Muhammad Raznisyafiq Razak +5 more
doaj +2 more sources
Evaluation of methods for the concentration and extraction of viruses from sewage in the context of metagenomic sequencing [PDF]
, 2017 Viral sewage metagenomics is a novel field of study used for surveillance, epidemiological studies, and evaluation of waste water treatment efficiency. In raw sewage human waste is mixed with household, industrial and drainage water, and virus particles ...
Aarestrup, Frank Møller +12 more
core +10 more sources