RNA preservation agents and nucleic acid extraction method bias perceived bacterial community composition. [PDF]
PLoS ONE, 2015 Bias is a pervasive problem when characterizing microbial communities. An important source is the difference in lysis efficiencies of different populations, which vary depending on the extraction protocol used.
Ann McCarthy +3 more
doaj +1 more source
Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality? [PDF]
, 2016 Background: Advanced genomic techniques such as Next-Generation-Sequencing (NGS) and gene expression profiling, including NanoString, are vital for the development of personalised medicines, as they enable molecular disease classification.
A Kalmar +31 more
core +1 more source
Assessment of RNA extraction protocols from cladocerans.
PLoS ONE, 2022 The usage of cladocerans as non-model organisms in ecotoxicological and risk assessment studies has intensified in recent years due to their ecological importance in aquatic ecosystems.
Muhammad Raznisyafiq Razak +5 more
doaj +2 more sources
Rapid bedside inactivation of Ebola virus for safe nucleic acid tests [PDF]
, 2016 Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport and high-throughput or rapid diagnostic testing during an outbreak.
Bragstad, Karoline +9 more
core +2 more sources
Extracting Small RNAs from Human Biological Fluids for Subsequent Next-Generation Sequencing
Креативная хирургия и онкология, 2019 A number of questions arise when choosing methods for experiments related to next-generation sequencing. On the one hand, while working with RNA extraction, added reagents and their residues can often inhibit sensitive chemicals with which the sequential
O. A. Beylerli +2 more
doaj +1 more source
Plant Methods, 2023 Background High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli.
Shina Sasi +7 more
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cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. [PDF]
, 2001 Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-
Centre Gunnels Wood +7 more
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Optimization of phenol-chloroform RNA extraction
MethodsX, 2018 Accurate and reliable analysis of gene expression depends on the extraction of pure and high-quality RNA. However, while the conventional phenol-chloroform RNA extraction is preferable over silica-based columns, particularly when cost is a concern or ...
Lee S. Toni +6 more
doaj +1 more source
A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenografts. [PDF]
, 2016 Next-generation sequencing (NGS) can identify and validate new biomarkers of cancer onset, progression and therapy resistance. Substantial archives of formalin-fixed, paraffin-embedded (FFPE) cancer samples from patients represent a rich resource for ...
Adamian, Yvess +12 more
core +2 more sources
MethodsX, 2018 RNA and DNA extraction is a requirement for the study of gene expression and has an increasingly important role in genetic studies of all fleshy fruits. RNA and DNA extraction is difficult in kiwifruit due to the significant amount of polysaccharides and
Mansour Afshar-Mohammadian +2 more
doaj +1 more source