Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality? [PDF]
, 2016 Background: Advanced genomic techniques such as Next-Generation-Sequencing (NGS) and gene expression profiling, including NanoString, are vital for the development of personalised medicines, as they enable molecular disease classification.
A Kalmar +31 more
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Extracting Small RNAs from Human Biological Fluids for Subsequent Next-Generation Sequencing
Креативная хирургия и онкология, 2019 A number of questions arise when choosing methods for experiments related to next-generation sequencing. On the one hand, while working with RNA extraction, added reagents and their residues can often inhibit sensitive chemicals with which the sequential
O. A. Beylerli +2 more
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RNA preservation agents and nucleic acid extraction method bias perceived bacterial community composition. [PDF]
PLoS ONE, 2015 Bias is a pervasive problem when characterizing microbial communities. An important source is the difference in lysis efficiencies of different populations, which vary depending on the extraction protocol used.
Ann McCarthy +3 more
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Rapid bedside inactivation of Ebola virus for safe nucleic acid tests [PDF]
, 2016 Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport and high-throughput or rapid diagnostic testing during an outbreak.
Bragstad, Karoline +9 more
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A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenografts. [PDF]
, 2016 Next-generation sequencing (NGS) can identify and validate new biomarkers of cancer onset, progression and therapy resistance. Substantial archives of formalin-fixed, paraffin-embedded (FFPE) cancer samples from patients represent a rich resource for ...
Adamian, Yvess +12 more
core +2 more sources
Assessment of RNA extraction protocols from cladocerans.
PLoS ONE, 2022 The usage of cladocerans as non-model organisms in ecotoxicological and risk assessment studies has intensified in recent years due to their ecological importance in aquatic ecosystems.
Muhammad Raznisyafiq Razak +5 more
doaj +2 more sources
An improved protocol for small RNA library construction using High Definition adapters [PDF]
, 2015 Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin +28 more
core +1 more source
Evaluation of methods for the concentration and extraction of viruses from sewage in the context of metagenomic sequencing [PDF]
, 2017 Viral sewage metagenomics is a novel field of study used for surveillance, epidemiological studies, and evaluation of waste water treatment efficiency. In raw sewage human waste is mixed with household, industrial and drainage water, and virus particles ...
Aarestrup, Frank Møller +12 more
core +6 more sources
A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses [PDF]
, 2005 http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010&lng=es&nrm=isoPrunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family.
Baeza-Yates, Ricardo +9 more
core +2 more sources
Plant Methods, 2023 Background High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli.
Shina Sasi +7 more
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