Results 31 to 40 of about 1,042,593 (334)

DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications

Plant Methods, 2023
Background High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli.
Shina Sasi, Saranya Krishnan, Preshobha Kodackattumannil, Aysha AL Shamisi, Maitha Aldarmaki, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri   +7 more
doaj   +1 more source

A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]

, 2012
With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood, Jie ePeng, Julin N Maloof, Lauren R Headland, Neelima R Sinha, Ravi eKumar, Seisuke eKimura, Seisuke eKimura, Yasunori eIchihashi   +8 more
core   +2 more sources

Comparative analysis and innovation of a simple and rapid method for high-quality RNA and DNA extraction of kiwifruit

MethodsX, 2018
RNA and DNA extraction is a requirement for the study of gene expression and has an increasingly important role in genetic studies of all fleshy fruits. RNA and DNA extraction is difficult in kiwifruit due to the significant amount of polysaccharides and
Mansour Afshar-Mohammadian, Mohammad Hossein Rezadoost, Seyyed Fatemeh Fallah   +2 more
doaj   +1 more source

Big data from small tissues: extraction of high-quality RNA for RNA-sequencing from different oilseed Brassica seed tissues during seed development

Plant Methods, 2020
Background Obtaining high-quality RNA for gene expression analyses from different seed tissues is challenging due to the presence of various contaminants, such as polyphenols, polysaccharides and lipids which interfere with RNA extraction methods.
Laura Siles, Peter Eastmond, Smita Kurup
doaj   +1 more source

cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. [PDF]

, 2001
Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-
Centre Gunnels Wood, Irene M. Monahan, Joseph A. Mangan, Martin J. Everett, Ming-shi Li, Philip D. Butcher, Simon J. Waddell, Steve L. Martin   +7 more
core   +3 more sources

A simple phenol-free isolation method for high-quality RNA from bilberry

MethodsX, 2021
High-quality RNA is required for accurate gene expression and transcriptome analysis. The current methods of RNA extraction from berry fruits are either time-consuming or expensive.
Kai Wang, Mengxia Liu, Fuqiang Cui, Fred Asiegbu   +3 more
doaj   +1 more source

Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach [PDF]

, 2019
Sequencing very long stretches of the HIV-1 genome can advance studies on virus evolution and in vivo recombination but remains technically challenging.
Demecheleer, Els, Hardy, Jarryt, Hebberecht, Laura, Mortier, Virginie, Schauvliege, Marlies, Staelens, Delfien, Vancoillie, Leen, Verhofstede, Chris   +7 more
core   +1 more source

Extraction of high-quality RNA from mouse pancreatic tumors

MethodsX, 2020
Extraction of high-quality RNA from pancreatic tumors for sequencing purposes is technically challenging, as the pancreas is an organ rich in ribonucleases.
Kranthi Kumar Chougoni, Steven R. Grossman   +1 more
doaj   +1 more source

Preparation of nuclear matrices from cultured cells: subfractionation of nuclei in situ [PDF]

, 1984
Analyses of the different structural systems of the nucleus and the proteins associated with them pose many problems. Because these systems are largely overlapping, in situ localization studies that preserve the in vivo location of proteins and cellular ...
Deppert, Wolfgang, Staufenbiel, Matthias
core   +2 more sources

An improved protocol for small RNA library construction using High Definition adapters [PDF]

, 2015
Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin, Baran, Bushati, Edgar, Git, Hafner, He, Jagadeeswaran, Jayaprakash, Kawano, Mallory, Mortazavi, Munafó, Pease, Prüfer, Raabe, Reddy, Song, Sorefan, Stocks, Sun, Szittya, Szittya, Tian, Vigneault, Viollet, Willenbrock, Zhang, Zhuang   +28 more
core   +1 more source