Results 31 to 40 of about 1,068,109 (284)
A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]
, 2012 With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood +8 more
core +2 more sources
Optimization of phenol-chloroform RNA extraction
MethodsX, 2018 Accurate and reliable analysis of gene expression depends on the extraction of pure and high-quality RNA. However, while the conventional phenol-chloroform RNA extraction is preferable over silica-based columns, particularly when cost is a concern or ...
Lee S. Toni +6 more
doaj +1 more source
MethodsX, 2018 RNA and DNA extraction is a requirement for the study of gene expression and has an increasingly important role in genetic studies of all fleshy fruits. RNA and DNA extraction is difficult in kiwifruit due to the significant amount of polysaccharides and
Mansour Afshar-Mohammadian +2 more
doaj +1 more source
An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing [PDF]
, 2017 Background: Formalin-fixed paraffin embedded (FFPE) tissue constitutes a vast treasury of samples for biomedical research. Thus far however, extraction of RNA from FFPE tissue has proved challenging due to chemical RNA–protein crosslinking and RNA ...
Amini, Parisa +5 more
core +1 more source
Plant Methods, 2020 Background Obtaining high-quality RNA for gene expression analyses from different seed tissues is challenging due to the presence of various contaminants, such as polyphenols, polysaccharides and lipids which interfere with RNA extraction methods.
Laura Siles, Peter Eastmond, Smita Kurup
doaj +1 more source
cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. [PDF]
, 2001 Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-
Centre Gunnels Wood +7 more
core +3 more sources
Extraction of high-quality RNA from mouse pancreatic tumors
MethodsX, 2020 Extraction of high-quality RNA from pancreatic tumors for sequencing purposes is technically challenging, as the pancreas is an organ rich in ribonucleases.
Kranthi Kumar Chougoni +1 more
doaj +1 more source
A simple phenol-free isolation method for high-quality RNA from bilberry
MethodsX, 2021 High-quality RNA is required for accurate gene expression and transcriptome analysis. The current methods of RNA extraction from berry fruits are either time-consuming or expensive.
Kai Wang +3 more
doaj +1 more source
Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction
MethodsX, 2019 Caenorhabditis elegans is a well-established laboratory animal model and has been widely used in biological research. However, it is still a challenge to obtain a good amount of quality RNA from a limited number of C. elegans for gene expression studies.
Leming Jiang +6 more
doaj +1 more source
Evaluation of Existing Methods for Human Blood mRNA Isolation and Analysis for Large Studies. [PDF]
PLoS ONE, 2016 Prior to implementing gene expression analyses from blood to a larger cohort study, an evaluation to set up a reliable and reproducible method is mandatory but challenging due to the specific characteristics of the samples as well as their collection ...
Anke Meyer +6 more
doaj +1 more source