Results 41 to 50 of about 1,068,109 (284)

Amniotic fluid from healthy term pregnancies does not harbor a detectable microbial community [PDF]

, 2018
Recent studies have conflicting data regarding the presence of intra-amniotic microbiota. Viral communities are increasingly recognized as important although overlooked components of the human microbiota.
Holtz, Lori R, Lim, Efrem S, Rodriquez, Cynthia   +2 more
core   +2 more sources

Comparison of Simple RNA Extraction Methods for Molecular Diagnosis of Hepatitis C Virus in Plasma

Diagnostics, 2022
Nucleic acid extraction from biological samples is an important step for hepatitis C virus (HCV) diagnosis. However, such extractions are mostly based on silica-based column methodologies, which may limit their application for on-site diagnosis. A simple,
Sayamon Hongjaisee, Yosita Jabjainai, Suthasinee Sakset, Kanya Preechasuth, Nicole Ngo-Giang-Huong, Woottichai Khamduang   +5 more
doaj   +1 more source

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A., Bahkali, A.H., Moslem, M.A., Wit, P.J.G.M., de   +3 more
core   +2 more sources

RT‐PCR–Based Detection of Bone Marrow Involvement in Ewing Sarcoma: Diagnostic Validity and Prognostic Relevance

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background In Ewing sarcoma (EwS), metastases, including those to bone marrow (BM), are the main factors influencing prognosis. Although reverse transcription polymerase chain reaction (RT‐PCR) offers greater sensitivity, the current EWING protocol defines BM metastases solely using light microscopic detection.
Thanh Pham, Andreas Ranft, Victor Rechl, Susanne Jabar, Wolfgang Hartmann, Kim Falkenberg, Heribert Jürgens, Norbert Graf, Torsten Keßler, Markus Metzler, Thomas Klingebiel, Thorsten Simon, Martin Benesch, Uta Dirksen   +13 more
wiley   +1 more source

Modification of a commercial dna extraction kit to simultaneously recover rna, safely and rapidly, and to assess molecular biomass of the total and the active part of microbial communities, from soils with diverse mineralogy and carbon content : S11.04-P -15 [PDF]

, 2012
We have modified a commercial DNA extraction kit for soil to simultaneously co-extract RNA. In this new procedure RNA and DNA are separated by two selective purifications in cascade without the need of DNAase or RNAse digestion.
Amenc, Laurie, Blanchart, Eric, Legname, Elvire, Pablo, Anne-laure, Plassard, Claude, Recous, Sylvie, Robin, Agnès, Tournier, Estelle   +7 more
core   +1 more source

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

A Comprehensive High-Quality DNA and RNA Extraction Protocol for a Range of Cultivars and Tissue Types of the Woody Crop Avocado

Plants, 2022
High-quality DNA and RNA forms the basis of genomic and genetic investigations. The extraction of DNA and RNA from woody trees, like avocado (Persea americana Mill.), is challenging due to compounds which interact with nucleic acids and influence ...
Onkar Nath, Stephen J. Fletcher, Alice Hayward, Lindsay M. Shaw, Rimjhim Agarwal, Agnelo Furtado, Robert J. Henry, Neena Mitter   +7 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Development of Magnetic-Silica Particles and In-house Buffers Kit for SARS-CoV-2 and CDV RNA Extraction

Indonesian Journal of Chemistry
Since the end of 2019, COVID-19 pandemic caused by the novel SARS-CoV-2 has become a serious problem for the world. Accurate and rapid techniques in testing and tracing are needed to control the virus spreading.
Ahadi Damar Prasetya, Muflikhah Muflikhah, Wildan Zakiah Lubis, Andon Insani, Grace Tjungirai Sulungbudi, Mujamilah Mujamilah, Uus Saepulloh   +6 more
doaj   +1 more source

The phenazine pyocyanin is a terminal signalling factor in the quorum sensing network of Pseudomonas aeruginosa [PDF]

, 2006
Certain members of the fluorescent pseudomonads produce and secrete phenazines. These heterocyclic, redox-active compounds are toxic to competing organisms, and the cause of these antibiotic effects has been the focus of intense research efforts.
Dietrich, Lars E. P., Newman, Dianne K., Petersen, Ashley, Price-Whelan, Alexa, Whiteley, Marvin   +4 more
core   +1 more source