Culture-Independent Detection of Poliovirus in Stool Samples by Direct RNA Extraction
Microbiology Spectrum, 2021 Laboratory surveillance for poliovirus (PV) relies on virus isolation by cell culture to identify PV in stool specimens from acute flaccid paralysis (AFP) cases.
Chelsea Harrington +8 more
doaj +1 more source
Amniotic fluid from healthy term pregnancies does not harbor a detectable microbial community [PDF]
, 2018 Recent studies have conflicting data regarding the presence of intra-amniotic microbiota. Viral communities are increasingly recognized as important although overlooked components of the human microbiota.
Holtz, Lori R +2 more
core +2 more sources
BMC Biotechnology, 2018 Background microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential.
Rikki A. M. Brown +5 more
doaj +1 more source
Phytochrome activates the plastid-encoded RNA polymerase for chloroplast biogenesis via nucleus-to-plastid signaling. [PDF]
, 2019 Light initiates chloroplast biogenesis by activating photosynthesis-associated genes encoded by not only the nuclear but also the plastidial genome, but how photoreceptors control plastidial gene expression remains enigmatic.
Blaha, Gregor M +6 more
core +2 more sources
RNAseq reveals hydrophobins that are involved in the adaptation of aspergillus nidulans to lignocellulose [PDF]
, 2016 Background Sugarcane is one of the world’s most profitable crops. Waste steam-exploded sugarcane bagasse (SEB) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels.
Brown, Neil Andrew +7 more
core +4 more sources
Dimerisation of HIV-2 genomic RNA is linked to efficient RNA packaging, normal particle maturation and viral infectivity. [PDF]
, 2007 BACKGROUND: Retroviruses selectively encapsidate two copies of their genomic RNA, the Gag protein binding a specific RNA motif in the 5' UTR of the genome.
Andrew ML Lever (60785) +3 more
core +3 more sources
High-quality total RNA extraction from early-stage lamprey embryos
BioTechniques, 2023 High-purity total RNA extraction from animal embryos is essential for transcriptome analyses. lampreys, together with hagfish, are the only extant jawless vertebrates or cyclostomes and are thus key organisms for EvoDevo studies.
Fumiaki Sugahara, Juan Pascual-Anaya
doaj +1 more source
RNA extraction for RNA sequencing of archival renal tissues [PDF]
Scandinavian Journal of Clinical and Laboratory Investigation, 2016 Next generation sequencing (NGS) and especially ribonucleic acid (RNA) sequencing is a powerful tool to acquire insights into molecular disease mechanisms. Therefore, it is of interest to optimize methods for RNA extraction from archival, formalin fixed and paraffin embedded (FFPE) tissues.
Christian Beisland +4 more
openaire +3 more sources
Investigation into the relationship between ethylene and sulfur assimilation in Arabidopsis thaliana and onion (Allium cepa L.) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science (with Honours) in Biochemistry at Massey University [PDF]
, 2006 The phytohormone ethylene (C2H4) mediates the adaptive responses of plants to various nutrient deficiencies including iron (Fe)-deficiency, phosphorus (P)-deficiency and potassium (K)-deficiency.
Sanggang, Fiona Ann
core
A Combined RNA Preservation and Extraction Protocol for Gene Expression Studies in Cacao Beans
Frontiers in Plant Science, 2020 Despite the high economic importance of cacao beans, few RNA-based studies have been conducted on this plant material and hence no optimal RNA-extraction has been reported.
Jocelyn De Wever +13 more
doaj +1 more source