Results 61 to 70 of about 356,994 (139)

iSeqQC: a tool for expression-based quality control in RNA sequencing. [PDF]

, 2020
BACKGROUND: Quality Control in any high-throughput sequencing technology is a critical step, which if overlooked can compromise an experiment and the resulting conclusions.
Ertel, Adam, Feldman, George, Fortina, Paolo, Kumar, Gaurav, Kupper, Joan   +4 more
core   +1 more source

Efficient oil palm total RNA extraction with a total RNA extraction kit

Genetics and Molecular Research, 2014
Oil palm tissues are rich in polyphenols, polysaccharides and secondary metabolites; these can co-precipitate with RNA, causing problems for downstream applications. We compared two different methods (one conventional and a kit-based method - Easy-Blue(TM) Total RNA Extraction Kit) to isolate total RNA from leaves, roots and shoot apical meristems of ...
S. H. Habib, H. Kausar, Halimi Mohd Saud
openaire   +3 more sources

A thin layer angiogenesis assay: a modified basement matrix assay for assessment of endothelial cell differentiation [PDF]

, 2014
BACKGROUND: Basement matrices such as Matrigel™ and Geltrex™ are used in a variety of cell culture assays of anchorage-dependent differentiation including endothelial cell tube formation assays.
A Li, AD Pierce, Andrew Hibbert, Ashton Faulkner, CA Staton, CA Staton, CA Staton, Caroline Wheeler-Jones, CE Clarkin, David Bishop-Bailey, E Garonna, F Vegran, G Wu, JJ Logie, L Piqueras, LS Cooley, M Ma, M Michaelis, M Schleicher, ME Capozzi, O Coutelle, RM Arantes, Robert Purcell, S Muses, Sally Latham, Scott Thomson, W Pendergrass, Wendy L Hall, X Zhao   +28 more
core   +1 more source

Enhancing Potato Virus X Diagnosis: Chelex ™ 100 Resin RNA Extraction Method Evaluation

Journal of Experimental and Molecular Biology
Potato Virus X (PVX) is a prevalent pathogen affecting potato crops, leading to substantial yield losses. This study investigates the efficacy of Chelex™ 100 resin RNA extraction method for PVX detection, through RT-qPCR analysis. We evaluate RNA yield,
Elizabeth Mendez, Juan David Saavedra, Fabio Ancizar Aristizabal Gutierrez   +2 more
doaj   +1 more source

Nucleoprotein content of "L" strain of Staphylococcus aureus [PDF]

, 1950
Thesis (M.A.)--Boston ...
Fish, Charles Austin
core   +1 more source

Antiviral RNA Interference against Orsay Virus Is neither Systemic nor Transgenerational in Caenorhabditis elegans. [PDF]

, 2015
UNLABELLED: Antiviral RNA-mediated silencing (RNA interference [RNAi]) acts as a powerful innate immunity defense in plants, invertebrates, and mammals. In Caenorhabditis elegans, RNAi is systemic; i.e., RNAi silencing signals can move between cells and ...
Ashe, Alyson, Le Pen, J?r?mie, Miska, Eric A., Sarkies, Peter, Tanguy, M?lanie   +4 more
core   +5 more sources

TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies [PDF]

Journal of Sciences, Islamic Republic of Iran, 2014
RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods.
M. Pahlevan Kakhki
doaj  

Comparative analysis and innovation of a simple and rapid method for high-quality RNA and DNA extraction of kiwifruit

MethodsX, 2018
RNA and DNA extraction is a requirement for the study of gene expression and has an increasingly important role in genetic studies of all fleshy fruits. RNA and DNA extraction is difficult in kiwifruit due to the significant amount of polysaccharides and
Mansour Afshar-Mohammadian, Mohammad Hossein Rezadoost, Seyyed Fatemeh Fallah   +2 more
doaj  

Simple Method for Efficient RNA Extraction From Arabidopsis Embryos

Bio-Protocol
Plant embryos are contained within seeds. Isolating them is crucial when endosperm and seed coat tissues interfere with the study of mutant genetic functions due to differing genotypes between maternal and embryonic tissues.
Fernanda Marchetti, Gabriela Pagnussat, Eduardo Zabaleta   +2 more
doaj   +1 more source

Reliable approaches to extract high-integrity RNA from skin and other pertinent tissues used in pain research

PAIN Reports, 2020
. Introduction:. Comprehensive mRNA sequencing is a powerful tool for conducting unbiased, quantitative differential gene expression analysis. However, the reliability of these data is contingent on the extraction of high-quality RNA from samples ...
Peter M. LoCoco, Jacob T. Boyd, Claudia M. Espitia Olaya, Ashley R. Furr, Dawn K. Garcia, Korri S. Weldon, Yi Zou, Erin Locke, Alejandro Tobon, Zhao Lai, Shivani B. Ruparel, Nikita B. Ruparel, Kenneth M. Hargreaves   +12 more
doaj   +1 more source