Results 71 to 80 of about 1,056,288 (288)

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

A Combined RNA Preservation and Extraction Protocol for Gene Expression Studies in Cacao Beans

Frontiers in Plant Science, 2020
Despite the high economic importance of cacao beans, few RNA-based studies have been conducted on this plant material and hence no optimal RNA-extraction has been reported.
Jocelyn De Wever, Jocelyn De Wever, Dieter Tulkens, Dieter Tulkens, Jan Verwaeren, Helena Everaert, Helena Everaert, Hayley Rottiers, Hayley Rottiers, Koen Dewettinck, Steve Lefever, Steve Lefever, Steve Lefever, Kathy Messens   +13 more
doaj   +1 more source

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

The phenazine pyocyanin is a terminal signalling factor in the quorum sensing network of Pseudomonas aeruginosa [PDF]

, 2006
Certain members of the fluorescent pseudomonads produce and secrete phenazines. These heterocyclic, redox-active compounds are toxic to competing organisms, and the cause of these antibiotic effects has been the focus of intense research efforts.
Dietrich, Lars E. P., Newman, Dianne K., Petersen, Ashley, Price-Whelan, Alexa, Whiteley, Marvin   +4 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Enhancing Potato Virus X Diagnosis: Chelex ™ 100 Resin RNA Extraction Method Evaluation

Journal of Experimental and Molecular Biology
Potato Virus X (PVX) is a prevalent pathogen affecting potato crops, leading to substantial yield losses. This study investigates the efficacy of Chelex™ 100 resin RNA extraction method for PVX detection, through RT-qPCR analysis. We evaluate RNA yield,
Elizabeth Mendez, Juan David Saavedra, Fabio Ancizar Aristizabal Gutierrez   +2 more
doaj   +1 more source

Detection of clinically relevant yeast in blood: evaluation of methods for RNA extraction and amplification [PDF]

, 2004
Objectives: To evaluate different methods of extraction andpurification of RNA from yeast in blood samples to be used inmolecular-based diagnosis. Methods: Different combinations ofcommercially available kits were tested with blood samplesartificially ...
Fátima de Melo Azevedo, Patricia Severino, Vanda Dolabela de Magalhães   +2 more
core  

Regulation of neutrophil senescence by microRNAs [PDF]

, 2011
Neutrophils are rapidly recruited to sites of tissue injury or infection, where they protect against invading pathogens. Neutrophil functions are limited by a process of neutrophil senescence, which renders the cells unable to respond to chemoattractants,
A Bonauer, A Lee, AD Kennedy, AS Cowburn, AW Ford-Hutchinson, C Haslett, C Martin, CH Wang, CL Andersen, DP Bartel, E Berezikov, E Tsitsiou, EP Cronkite, F Bazzoni, F Colotta, F Fazi, Frank R. DeLeo, GM Bokoch, H Su, H Xia, I Sabroe, I Sabroe, J Davidson-Moncada, J Savill, J Yu, James W. Catto, JB Johnnidis, JJ Lum, JL Mott, Jon R. Ward, JR Ward, LR Prince, M Merkerova, M Scherr, Marta Milo, MK Whyte, MK Whyte, Moira K. B. Whyte, OD Weiner, P Scapini, PA Davoren, Paul R. Heath, PT Nelson, R Chhabra, R Yi, RE Stringer, RW Watson, S Huang, S Radom-Aizik, S Slezak, SA Renshaw, SD Kobayashi, SD Kobayashi, Stephen A. Renshaw, W Filipowicz, W Huang da, WC Liles, WP Tsang, Y Lee, Y Wang, YZ Pan   +60 more
core   +5 more sources

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source