miRNAs as Regulators of Antidiabetic Effects of Fucoidans [PDF]
open access articleDiabetes mellitus is a metabolic disease with a high mortality rate worldwide. MicroRNAs (miRNAs), and other small noncoding RNAs, serve as endogenous gene regulators through binding to specific sequences in RNA and modifying gene ...
Arroo, R. R. J.+8 more
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Comparison of RNA Extraction Methods for the Identification of Grapevine fan leaf virus
Introduction: To now, more than 70 viral diseases have been reported from grapevine. Serological methods are regular diagnostic tools of grapevine viruses, however, their sensitivity has affected by seasonal fluctuations of the virus.
Z. Gholampour, M. Zakiaghl
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Comparison of protein quantification and extraction methods suitable for E-coli cultures [PDF]
Many different extraction and analysis methods exist to determine the protein fraction of microbial cells. For metabolic engineering purposes it is important to have precise and accurate measurements.
De Mey, Marjan+5 more
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Rapid RNA Extraction from Eucalyptus tree and its down processing for cloning of dehydrin genes
Background: RNA extraction from tree species like Eucalyptus is very tedious and difficult task. In this study a very short and efficient method of RNA extraction from Eucalyptus has been described.
Ghulam Zahara Jahangir+3 more
doaj
A simple phenol-free isolation method for high-quality RNA from bilberry
High-quality RNA is required for accurate gene expression and transcriptome analysis. The current methods of RNA extraction from berry fruits are either time-consuming or expensive.
Kai Wang+3 more
doaj
Characterization of a disease-associated mutation affecting a putative splicing regulatory element in intron 6b of the cystic fibrosis transmembrane conductance regulator (CFTR) gene [PDF]
Cystic fibrosis (CF) is a common recessive disorder caused by >1600 mutations in the CF transmembrane conductance regulator (CFTR) gene. About 13% of CFTR mutations are classified as “splicing mutations,” but for almost 40% of these, their role in ...
BAFFICO AM+8 more
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Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus [PDF]
The methyl-CpG binding domain (MBD) proteins are key molecules in the interpretation of DNA methylation signals leading to gene silencing. We investigated their binding specificity at the constitutively methylated region of a CpG island containing the ...
Auriol, Emilie+3 more
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Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction
Caenorhabditis elegans is a well-established laboratory animal model and has been widely used in biological research. However, it is still a challenge to obtain a good amount of quality RNA from a limited number of C. elegans for gene expression studies.
Leming Jiang+6 more
doaj
From fix to fit into the autoptic human brains. [PDF]
Formalin-fixed, paraffinembedded (FFPE) human brain tissues are very often stored in formalin for long time. Formalin fixation reduces immunostaining, and the DNA/RNA extraction from FFPE brain tissue becomes suboptimal.
Lavezzi, A., Paradiso, B, Simonato, M
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High Quality RNA Isolation from Leaf, Shell, Root Tissues and Callus of Hazelnut (Corylus avellana L.) [PDF]
Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most important nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explaining their biosynthesis ...
Hossein Khosravi+2 more
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