RNA Extraction From Mammalian Tissues
, 2007 Purification of intact RNA is the primary step of many molecular biology techniques, including Northern blotting, RNase protection, quantitative polymerase chain reaction, and microarray assays. RNA extraction is typically conducted using either a phenol-chloroform or a solid phase method.
Peirson, S, Butler, J
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Evaluation of three RNA extraction methods from three cultivars of Malaysian upland rice [PDF]
, 2016 Rice (Oryza sativa L.) can be divided into two major categories, which is upland rice and lowland rice. Apart from being the staple food for more than half of the world population, it is also known as model plant for functional genomics study.
Arif, S. M. +3 more
core
Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits [PDF]
, 2020 The success of fundamental and applied nucleic acid (NA) research depends on NA purity, but obtaining pure NAs from raw, unprocessed samples is challenging.
Ismagilov, Rustem F. +2 more
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Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue [PDF]
, 2016 Background Laser capture microdissection offers unique possibilities for the isolation of specific cell populations or histological structures. However, isolation of RNA from microdissected tissue is challenging due to degradation and minimal yield of
Kolijn, K. (Kimberley) +1 more
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Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
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Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions [PDF]
, 2019 Fungal infections, ranging from superficial to life-threatening infections, represent a major public health problem that affects 25% of the worldwide population.
Coucke, Paul +3 more
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Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
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Investigation into the relationship between ethylene and sulfur assimilation in Arabidopsis thaliana and onion (Allium cepa L.) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science (with Honours) in Biochemistry at Massey University [PDF]
, 2006 The phytohormone ethylene (C2H4) mediates the adaptive responses of plants to various nutrient deficiencies including iron (Fe)-deficiency, phosphorus (P)-deficiency and potassium (K)-deficiency.
Sanggang, Fiona Ann
core
Function‐driven design of a surrogate interleukin‐2 receptor ligand
FEBS Letters, EarlyView.Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang +9 more
wiley +1 more source
Total RNA Extraction from Tardigrades [PDF]
Cold Spring Harbor Protocols, 2018 Purification of high-quality total RNA from specimens is essential for many molecular techniques. In tardigrades (water bears), disruption of the cuticle is an important step in obtaining a good yield that is representative of all tissues of the animal.
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