Results 81 to 90 of about 1,068,109 (284)

RNA extraction for RNA sequencing of archival renal tissues [PDF]

Scandinavian Journal of Clinical and Laboratory Investigation, 2016
Next generation sequencing (NGS) and especially ribonucleic acid (RNA) sequencing is a powerful tool to acquire insights into molecular disease mechanisms. Therefore, it is of interest to optimize methods for RNA extraction from archival, formalin fixed and paraffin embedded (FFPE) tissues.
Lea, Landolt, Hans-Peter, Marti, Christian, Beisland, Arnar, Flatberg, Oystein Solberg, Eikrem   +4 more
openaire   +2 more sources

Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus [PDF]

, 1996
The methyl-CpG binding domain (MBD) proteins are key molecules in the interpretation of DNA methylation signals leading to gene silencing. We investigated their binding specificity at the constitutively methylated region of a CpG island containing the ...
Auriol, Emilie, Billard, Lise-Marie, Magdinier, Frédérique, Dante, Robert   +3 more
core   +2 more sources

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

Enhancing Potato Virus X Diagnosis: Chelex ™ 100 Resin RNA Extraction Method Evaluation

Journal of Experimental and Molecular Biology
Potato Virus X (PVX) is a prevalent pathogen affecting potato crops, leading to substantial yield losses. This study investigates the efficacy of Chelex™ 100 resin RNA extraction method for PVX detection, through RT-qPCR analysis. We evaluate RNA yield,
Elizabeth Mendez, Juan David Saavedra, Fabio Ancizar Aristizabal Gutierrez   +2 more
doaj   +1 more source

Efficient oil palm total RNA extraction with a total RNA extraction kit

Genetics and Molecular Research, 2014
Oil palm tissues are rich in polyphenols, polysaccharides and secondary metabolites; these can co-precipitate with RNA, causing problems for downstream applications. We compared two different methods (one conventional and a kit-based method - Easy-Blue(TM) Total RNA Extraction Kit) to isolate total RNA from leaves, roots and shoot apical meristems of ...
S H, Habib, H M, Saud, H, Kausar
openaire   +2 more sources

Comparison of protein quantification and extraction methods suitable for E-coli cultures [PDF]

, 2008
Many different extraction and analysis methods exist to determine the protein fraction of microbial cells. For metabolic engineering purposes it is important to have precise and accurate measurements.
De Mey, Marjan, De Muynck, Cassandra, Lequeux, Gaspard, Maertens, Jo, Soetaert, Wim, Vandamme, Erick   +5 more
core   +2 more sources

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

RNA extraction and purification

, 2022
Protocol for RNA extraction from cell lines and purification for RNA-Seq.
Klimek, Christoph, Onstein, Svenja, Barbosa, Barbara, Wolf, Gernot   +3 more
openaire   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies [PDF]

Journal of Sciences, Islamic Republic of Iran, 2014
RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods.
M. Pahlevan Kakhki
doaj