Results 81 to 90 of about 356,994 (139)

Virtual in situs: Sequencing mRNA from cryo-sliced Drosophila embryos to determine genome-wide spatial patterns of gene expression [PDF]

open access: yes, 2013
Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development.
Combs, Peter A., Eisen, Michael B.
core   +4 more sources

RNA Extraction From Mammalian Tissues

open access: yes, 2007
Purification of intact RNA is the primary step of many molecular biology techniques, including Northern blotting, RNase protection, quantitative polymerase chain reaction, and microarray assays. RNA extraction is typically conducted using either a phenol-chloroform or a solid phase method.
Peirson, S, Butler, J
openaire   +4 more sources

Tomato prosystemin gene in other Solanaceae [PDF]

open access: yes, 2009
Systemin, an octadeca-peptide isolated from tomato, is a signalling molecule involved in local and systemic wound response. It regulates the activation of more than twenty defensive genes in tomato plants in response to herbivore attacks.
Araujo Burgos, Tania
core   +1 more source

Extraction of high-quality RNA from mouse pancreatic tumors

open access: yesMethodsX, 2020
Extraction of high-quality RNA from pancreatic tumors for sequencing purposes is technically challenging, as the pancreas is an organ rich in ribonucleases.
Kranthi Kumar Chougoni   +1 more
doaj  

Felis catus papillomavirus type 2 infection and skin cancer in domestic cats : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand [PDF]

open access: yes, 2016
Felis catus papillomavirus type 2 (FcaPV-2) is a virus which commonly infects the skin of domestic cats. While most infections are asymptomatic, there is growing evidence that FcaPV-2 may play a role in the development of a subset of feline cutaneous ...
Thomson, Neroli Anne
core  

Optimisation of laboratory methods for whole transcriptomic RNA analyses in human left ventricular biopsies and blood samples of clinical relevance.

open access: yesPLoS ONE, 2019
This study aimed to optimise techniques for whole transcriptome and small RNA analyses on clinical tissue samples from patients with cardiovascular disease.
Kerrie L Ford   +10 more
doaj   +1 more source

A High-Throughput RNA Extraction for Sprouted Single-Seed Barley (Hordeum vulgare L.) Rich in Polysaccharides

open access: yesPlants, 2016
Germinated seed from cereal crops including barley (Hordeum vulgare L.) is an important tissue to extract RNA and analyze expression levels of genes that control aspects of germination.
Abdur Rashid   +4 more
doaj   +1 more source

Steps in Metagenomics: Let’s Avoid Garbage in and Garbage Out [PDF]

open access: yes, 2015
Is metagenomics a revolution or a new fad? Metagenomics is tightly associated with the availability of next-generation sequencing in all its implementations.
Izard, Jacques
core   +1 more source

Assessment of the efficiency of virus extraction from food matrices and the frequency of occurrence of contaminated products in the retail network

open access: yesПищевые системы
Express detection of viruses, in particular, norovirus (NoV) and hepatitis A virus (HAV), is becoming an extremely important task for food safety control.
Yu. K. Yushina   +5 more
doaj   +1 more source

The effects of arbuscular mycorrhizal fungi (AMF) and Rhizophagus irregularis on soil microorganisms assessed by metatranscriptomics and metaproteomics

open access: yes, 2019
Arbuscular mycorrhizal fungi (AMF) form symbioses with approximately 80% of plant species and potentially benefit their hosts (e.g. nutrient acquisition) and the soil environment (e.g. soil aggregation).
Johnston, P.   +4 more
core   +1 more source

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