Results 1 to 10 of about 82,511 (219)

Synthesis of ATP derivatives of compounds of the mevalonate pathway (isopentenyl di- and triphosphate; geranyl di- and triphosphate, farnesyl di- and triphosphate, and dimethylallyl diphosphate) catalyzed by T4 RNA ligase, T4 DNA ligase and other ligases [PDF]

Biochemical Pharmacology, 2009
Compounds of the mevalonate pathway containing a terminal di- or triphosphate (mev-PP or mev-PPP) were tested as substrates of several enzyme ligases (T4 RNA ligase, T4 DNA ligase, firefly luciferase and other ligases) for the synthesis of ATP derivatives of the mev-pppA or mev-ppppA type.
Marı́a A. Günther Sillero, Anabel de Diego, Janeth E.F. Tavares, Joana A.D. Catanho da Silva, Francisco J. Pérez-Zúñiga, Antonio Sillero   +5 more
openalex   +5 more sources

Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase

Proceedings of the National Academy of Sciences of the United States of America, 2015
Significance Polynucleotide ligases are an ancient superfamily of nucleic acid repair enzymes that join 3′-OH and 5′-PO4 DNA or RNA ends. Ligases react with ATP or NAD+ to form a covalent enzyme–adenylate intermediate in which AMP is linked via a P–N ...
M. Unciuleac, Y. Goldgur, S. Shuman
semanticscholar   +1 more source

Characterization of a novel eukaryal nick-sealing RNA ligase from Naegleria gruberi

RNA: A publication of the RNA Society, 2015
The proteome of the amoebo-flagellate protozoan Naegleria gruberi is rich in candidate RNA repair enzymes, including 15 putative RNA ligases, one of which, NgrRnl, is a eukaryal homolog of Deinococcus radiodurans RNA ligase, DraRnl.
M. Unciuleac, S. Shuman
semanticscholar   +1 more source

CRL2LRRC41-Mediated DDX5 Ubiquitination Enhances Interaction with ELAVL1 Preventing NOG mRNA Degradation and Sustaining Proliferation and Migration of Human Spermatogonial Stem Cell-Like Cell Line

BMC Biology
Background Human spermatogonial stem cells (SSCs) exhibit a remarkable capacity for proliferation, crucial for sustaining spermatogenesis throughout life.
Bing Jiang, Kehan Wang, Haoyue Hu, Wenxin Gao, Cong Shen, Xia Chen, Xiaoyan Huang, Jun Yu, Yibo Wu, Bo Zheng   +9 more
doaj   +1 more source

Simple and efficient synthesis of 5′ pre-adenylated DNA using thermostable RNA ligase

Nucleic Acids Research, 2011
We report a simple method of enzymatic synthesis of pre-adenylated DNA linkers/adapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoautotrophicum (MthRnl).
A. Zhelkovsky, L. McReynolds
semanticscholar   +1 more source

Inhibition of acyl‐CoA synthetase long‐chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction

Molecular Oncology
Multiple myeloma (MM) is an incurable cancer of plasma cells with a 5‐year survival rate of 59%. Dysregulation of fatty acid (FA) metabolism is associated with MM development and progression; however, the underlying mechanisms remain unclear.
Connor S. Murphy, Heather Fairfield, Victoria E. DeMambro, Samaa Fadel, Carlos A. Gartner, Michelle Karam, Christian Potts, Princess Rodriguez, Ya‐Wei Qiang, Habib Hamidi, Xiangnan Guan, Calvin P. H. Vary, Michaela R. Reagan   +12 more
doaj   +1 more source

Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties

Nucleic Acids Research, 2005
We have recently sequenced the genome of a novel thermophilic bacteriophage designated as TS2126 that infects the thermophilic eubacterium Thermus scotoductus.
T. Blondal, Audur Thorisdottir, U. Unnsteinsdóttir, S. Hjorleifsdottir, Arnthór Ævarsson, S. Ernstsson, Ó. Fridjónsson, S. Skírnisdóttir, J. Wheat, A. Hermannsdottir, S. Sigurdsson, G. Hreggvidsson, A. Smith, J. Kristjánsson   +13 more
semanticscholar   +1 more source

RNA Substrate Specificity and Structure-guided Mutational Analysis of Bacteriophage T4 RNA Ligase 2*

Journal of Biological Chemistry, 2004
Here we report that bacteriophage T4 RNA ligase 2 (Rnl2) is an efficient catalyst of RNA ligation at a 3′-OH/5′-PO4 nick in a double-stranded RNA or an RNA·DNA hybrid.
J. Nandakumar, C. Ho, Christopher D. Lima, S. Shuman   +3 more
semanticscholar   +1 more source

R2D ligase: Unveiling a novel DNA ligase with surprising DNA‐to‐RNA ligation activity

Biotechnology Journal
DNA ligases catalyze bond formation in the backbone of nucleic acids via the formation of a phosphodiester bond between adjacent 5′ phosphates and 3′ hydroxyl groups on one strand of the duplex.
S. Gundesø, U. Rothweiler, Elise Heimland, Yvonne Piotrowski, Inger Kristine Rødum, Jenny Johansson Söderberg, Ildikó Moustaka Gábor, Terese Solstad, Adele Williamson, O. Lanes, Bernd Ketelsen Striberny   +10 more
semanticscholar   +1 more source

Additional file 2: of Three-dimensional structure model and predicted ATP interaction rewiring of a deviant RNA ligase 2

, 2015
Sandrine Moreira, Emmanuel Noutahi, Guillaume Lamoureux, Gertraud Burger   +3 more
openalex   +1 more source