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Cleavage of 3′-terminal adenosine by archaeal ATP-dependent RNA ligase
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Inhibition of the First Stage of RNA Ligase Reaction by ATP Analogues [PDF]
Abstract It was shown that most ATP analogues which am modified with respect to polyphosphate part of the molecule are competitive inhibitors of the first stage of the RNA ligase reaction. All investigated compounds, with the exception of AMP and ADP, from a covalent AMP-RNA ligase complex.
D. Labeiltyte, Benediktas Juodka
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To elucidate the role of the ribose moiety in substrate binding, various ATP derivatives modified in ribose moiety were studied as probes for the T4 RNA ligase first stage reaction. The kinetic parameters for competitive inhibition were determined. Inhibition experiments using substrate analogs demonstrated that the major binding determinants of ATP ...
B A, Iuodka+2 more
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The dependence of initial rate v0 of ATP--PPi exchange reaction catalyzed by RNA-ligase of bacteriophage T4 on the concentration of ATP(s), pyrophosphate (z) and Mgcl2 has been determined. The dependence of v0 on s and z described by the equation v0 = k-1k2E0/(k-1 + K2) (1 + K1/s + k2/z) has been obtained for the reaction of E + S in equilibrium ES in ...
S N, Zagrebel'nyĭ+2 more
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[Study of ATP-independent stages of reaction catalyzed by phage T4 RNA-ligase].
Molekuliarnaia biologiia, 1987The isotope exchange between [5'-32P]pAP and A(5')ppAp catalyzed by enzyme was shown not to take place in the absence of the acceptor; i. e. the necessity of the acceptor presence during the second step of the process was demonstrated. The isotope exchange reaction between [5'32P]pAp and (pA)5p was studied.
S N, Zagrebel'nyĭ, Iu P, Zernov
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E3 ligase ligand chemistries: from building blocks to protein degraders
Chemical Society Reviews, 2022Izidor Sosi+2 more
exaly