Results 31 to 40 of about 82,511 (219)

A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria

Bio-Protocol, 2018
Next generation high-throughput sequencing has enabled sensitive and unambiguous analysis of RNA populations in cells. Here, we describe a method for isolation and strand-specific sequencing of small RNA pools from bacteria that can be multiplexed to ...
Sukrit Silas, Nimit Jain, Michael Stadler, Becky Fu, Antonio Sanchez-Amat, Andrew Fire, Joshua Arribere   +6 more
doaj   +1 more source

Circular RNA ITCH suppresses proliferation, invasion, and glycolysis of ovarian cancer cells by up-regulating CDH1 via sponging miR-106a

Cancer Cell International, 2020
Background Accumulating data suggested that circular RNAs (circRNAs) played important roles in the development of human cancer. However, the potential mechanism of circRNAs in ovarian cancer remains unclear.
Chunli Lin, Xiaofeng Xu, Qiumin Yang, Lu Liang, Shulin Qiao   +4 more
doaj   +1 more source

Biochemical and structural insights into a 5’ to 3’ RNA ligase reveal a potential role in tRNA ligation

bioRxiv
ATP-grasp superfamily enzymes contain a hand-like ATP-binding fold and catalyze a variety of reactions using a similar catalytic mechanism. More than 30 protein families are categorized in this superfamily, and they are involved in a plethora of cellular
Yingjie Hu, Victor A Lopez, Hengyi Xu, James Pfister, B. Song, Kelly A. Servage, Masahiro Sakurai, Benjamin T. Jones, J. Mendell, Tao Wang, Jun Wu, A. Lambowitz, D. Tomchick, Krzysztof Pawłowski, V. Tagliabracci   +14 more
semanticscholar   +2 more sources

The spliceosome-activating complex: molecular mechanisms underlying the function of a pleiotropic regulator

Frontiers in Plant Science, 2012
Correct interpretation of the coding capacity of RNA polymerase II transcribed eukaryotic genes is determined by the recognition and removal of intronic sequences of pre-mRNAs by the spliceosome.
Csaba eKoncz, Femke edeJong, Femke edeJong, Nicolas eVillacorta, Dora eSzakonyi, Dora eSzakonyi, Zsuzsa eKoncz   +6 more
doaj   +1 more source

Caveat mutator: alanine substitutions for conserved amino acids in RNA ligase elicit unexpected rearrangements of the active site for lysine adenylylation

Nucleic Acids Research, 2020
Naegleria gruberi RNA ligase (NgrRnl) exemplifies the Rnl5 family of adenosine triphosphate (ATP)-dependent polynucleotide ligases that seal 3′-OH RNA strands in the context of 3′-OH/5′-PO4 nicked duplexes.
M. Unciuleac, Y. Goldgur, S. Shuman
semanticscholar   +1 more source

Characterisation and engineering of a thermophilic RNA ligase from Palaeococcus pacificus

Nucleic Acids Research
RNA ligases are important enzymes in molecular biology and are highly useful for the manipulation and analysis of nucleic acids, including adapter ligation in next-generation sequencing of microRNAs. Thermophilic RNA ligases belonging to the RNA ligase 3
Meghan Rousseau, Tifany Oulavallickal, Adele Williamson, Vic Arcus, Wayne M Patrick, Joanna Hicks   +5 more
semanticscholar   +1 more source

Structure, Function and Regulation of the Hsp90 Machinery

Biomedical Journal, 2013
Heat shock protein 90 (Hsp90) is an ATP-dependent molecular chaperone which is essential in eukaryotes. It is required for the activation and stabilization of a wide variety of client proteins and many of them are involved in important cellular pathways.
Jing Li, Johannes Buchner
doaj   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

mTORC1/S6K1 signaling promotes sustained oncogenic translation through modulating CRL3IBTK-mediated ubiquitination of eIF4A1 in cancer cells

eLife
Enhanced protein synthesis is a crucial molecular mechanism that allows cancer cells to survive, proliferate, metastasize, and develop resistance to anti-cancer treatments, and often arises as a consequence of increased signaling flux channeled to mRNA ...
Dongyue Jiao, Huiru Sun, Xiaying Zhao, Yingji Chen, Zeheng Lv, Qing Shi, Yao Li, Chenji Wang, Kun Gao   +8 more
doaj   +1 more source