Results 81 to 90 of about 2,070,961 (352)

Analysis of efficiently packaged defective interfering RNAs of murine coronavirus: localization of a possible RNA-packaging signal [PDF]

Journal of Virology, 1990
We have previously shown that most of the defective interfering (DI) RNA of mouse hepatitis virus (MHV) are not packaged into virions. We have now identified, after 21 serial undiluted passages of MHV, a small DI RNA, DIssF, which is efficiently packaged into virions.
Michael M. C. Lai, Kyoko Yokomori, Shinji Makino   +2 more
openaire   +3 more sources

In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8

BMC Microbiology, 2005
Background Φ8 is a bacteriophage containing a genome of three segments of double-stranded RNA inside a polyhedral capsid enveloped in a lipid-containing membrane. Plus strand RNA binds and is packaged by empty procapsids.
Mindich Leonard, Qiao Xueying, Qiao Jian
doaj   +1 more source

Functional RNAs: combined assembly and packaging in VLPs [PDF]

Nucleic Acids Research, 2016
We describe here a one pot RNA production, packaging and delivery system based on bacteriophage Qβ. We demonstrate a method for production of a novel RNAi scaffold, packaged within Qβ virus-like particles (VLPs). The RNAi scaffold is a general utility chimera that contains a functional RNA duplex with paired silencing and carrier sequences stabilized ...
Stefany Y. Holguin, Po-Yu Fang, Hung-Wei Yang, Lizzette M. Gómez Ramos, Chiaolong Hsiao, Loren Dean Williams, Jessica C. Bowman   +6 more
openaire   +2 more sources

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

Distinct binding interactions of HIV-1 Gag to Psi and non-Psi RNAs: Implications for viral genomic RNA packaging

RNA: A publication of the RNA Society, 2013
The mechanism underlying the selective packaging of genomic RNA into HIV-1 virions is not known. This paper provides important new biophysical insights into the nature of protein–RNA interactions responsible for HIV-1 genome packaging by quantifying the ...
Joseph A Webb, Christopher P Jones, L. Parent, I. Rouzina, K. Musier-Forsyth   +4 more
semanticscholar   +1 more source

ERBIN limits epithelial cell plasticity via suppression of TGF‐β signaling

FEBS Letters, EarlyView.
In breast and lung cancer patients, low ERBIN expression correlates with poor clinical outcomes. Here, we show that ERBIN inhibits TGF‐β‐induced epithelial‐to‐mesenchymal transition in NMuMG breast and A549 lung adenocarcinoma cell lines. ERBIN suppresses TGF‐β/SMAD signaling and reduces TGF‐β‐induced ERK phosphorylation.
Chao Li, Gerard van der Zon, Peter ten Dijke, Tong Shen   +3 more
wiley   +1 more source

Unpaired Guanosines in the 5′ Untranslated Region of HIV-1 RNA Act Synergistically To Mediate Genome Packaging

Journal of Virology, 2020
HIV-1 must package its RNA genome during virus assembly to generate infectious viruses. To better understand how HIV-1 packages its RNA genome, we examined the roles of RNA elements identified as binding sites for NC, a Gag-derived RNA-binding protein ...
Olga A. Nikolaitchik, Xayathed Somoulay, Jonathan M O Rawson, J. A. Yoo, V. Pathak, Wei-Shau Hu   +5 more
semanticscholar   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

Role of genome topology in the stability of viral capsids

Physical Review Research, 2023
We explore how the stability of RNA viruses depends on genome topology and interactions between RNA and the capsid proteins. RNA is modeled as a branched polymer with 12 attractive sites (packaging signals) that can form bonds with 12 icosahedrally ...
James Daniel Farrell, Jure Dobnikar, Rudolf Podgornik   +2 more
doaj   +1 more source

NXF1 and CRM1 nuclear export pathways orchestrate nuclear export, translation and packaging of murine leukaemia retrovirus unspliced RNA

RNA Biology, 2020
Cellular mRNAs are exported from the nucleus as fully spliced RNAs. Proofreading mechanisms eliminate unprocessed and irregular pre-mRNAs to control the quality of gene expression.
M. Mougel, C. Akkawi, Célia Chamontin, J. Feuillard, Lucie Pessel-Vivares, M. Socol, S. Lainé   +6 more
semanticscholar   +1 more source