Results 321 to 330 of about 991,885 (339)

RNA polymerase II transcription cycles

Current Opinion in Genetics & Development, 1993
RNA polymerase II interacts with transcription factors to initiate the synthesis of mRNA. These interactions are cyclic, involving multiple RNA polymerase subunits and general transcription factors. Phosphorylation of the RNA polymerase II carboxyl-terminal domain may regulate these interactions.
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[38] RNA polymerase II transcription in vitro

1991
Publisher Summary This chapter presents method for preparing nuclear extracts and describes assay procedures that can determine the quality of an extract for initiation by RNA polymerase II. Yeast nuclei arc isolated following lysis of spheroplasts, produced by degradation of cell walls with crude yeast lyric enzyme.
Peter M. Flanagan, Roger D. Kornberg, Raymond J. Kelleher, Neal F. Lue, Aled M. Edwards   +4 more
openaire   +3 more sources

RNA Polymerase: Structural Similarities Between Bacterial RNA Polymerase and Eukaryotic RNA Polymerase II

Journal of Molecular Biology, 2000
Bacterial RNA polymerase and eukaryotic RNA polymerase II exhibit striking structural similarities, including similarities in overall structure, relative positions of subunits, relative positions of functional determinants, and structures and folding topologies of subunits.
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[15] Yeast RNA polymerase II holoenzyme

1996
Publisher Summary This chapter discusses the present protocol for holoenzyme purification as an aid to further biochemical and molecular genetic analysis. This procedure entails a series of chromatographic steps, followed by gel filtration, which resolves an apparently homogeneous holoenzyme at the leading edge of a broad polymerase peak.
Young Joon Kim, Roger D. Kornberg, Stefan Björklund, Yang I. Li   +3 more
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Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences.

Journal of Molecular Biology, 1990
P. Bucher
semanticscholar   +1 more source

Causes and consequences of RNA polymerase II stalling during transcript elongation

Nature reviews. Molecular cell biology, 2020
Melvin Noé González, Daniel Blears, J. Svejstrup   +2 more
semanticscholar   +1 more source

[17] Phosphorylation of mammalian RNA polymerase II

1996
Abstract This chapter has described procedures for the phosphorylation of mammalian RNAP II with CKII and CTD kinases. The naturally occurring CKII site at the C terminus of the largest mammalian RNAP II subunit provides a convenient means to tag RNAP II with 32 P.
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RNA Polymerase II

Annual Review of Biochemistry, 1991
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Developmentally regulated transcription of mammalian telomeres by DNA-dependent RNA polymerase II

Nature Cell Biology, 2008
Stefan Schoeftner, M. Blasco
semanticscholar   +1 more source

The C-terminal domain of RNA polymerase II couples mRNA processing to transcription

Nature, 1997
S. McCracken, N. Fong, K. Yankulov, S. Ballantyne, G. Pan, J. Greenblatt, S. Patterson, M. Wickens, D. Bentley   +8 more
semanticscholar   +1 more source