Rna In Situ Protocol For Dna And Rna Probes
Author: Grace Panganiban This procedure was adapted from one originally described by Tautz and Pfeifle (1989). It makes use of digoxigenin labelled probes and alkaline phosphatase-conjugated anti-digoxigenin antibody fragments (Boehringer Mannheim Biochemicals) to detect the hybridizing RNA in whole embryos.
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Contributions of in situ hybridization of sRNA to the study on spatio-temporal gene expression in Hevea brasiliensis [PDF]
Since the dawn of the genomics era, much research has focused on functional studies of genes of interest. In situ hybridization is a method that can be used to precisely localize the expression of a gene in tissues and cells.
Alemanno, Laurence+6 more
core
Chemical Methylation of RNA and DNA Viral Genomes as a Probe of In Situ Structure [PDF]
Minoru Yamakawa+2 more
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In situ hybridization of immunoglobulin-specific RNA in single cells of the B lymphocyte lineage with radiolabelled DNA probes [PDF]
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Detection of Human Group a and C Rotaviruses in Pediatric Patients with Acute Gastroenteritis by Real TIME RT-PCR Assay: a Preliminary Study [PDF]
Rotavirus causes 25–55% of all hospital admissions for diarrhea and approximately 611.000 deaths every year in developing countries. Clinically, it is not possible to recognize the diarrhea caused by rotavirus and other infections.
Dewi, B. E. (Beti)+3 more
core
Thermodynamics of RNA/DNA hybridization in high density oligonucleotide microarrays
We analyze a series of publicly available controlled experiments (Latin square) on Affymetrix high density oligonucleotide microarrays using a simple physical model of the hybridization process.
Bolstad+16 more
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Rationale: Colorectal cancer (CRC) is one of the most prevalent oncological diseases with high mortality. Invasive optical (endoscopic) colono- scopy has been recognized as a golden standard for the CRC diagnostics. A promising area is the development of
Yuri D. Ivanov+21 more
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Preparation of a specific RNA probe for detection of Mycobacterium paratuberculosis and diagnosis of Johne's disease [PDF]
P Poupart+3 more
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Structural analysis of the 5′ domain of the HeLa 18S ribosomal RNA by chemical and enzymatic probing [PDF]
Valsan Mandiyan, M. Boublík
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Probing structural differences between native andin vitrotranscribedEscherichia colivaline transfer RNA: evidence for stable base modification-dependent conformers [PDF]
Wesley B. Derrick, Jack Horowitz
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